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Evaluation And Application Of Crispr-Cas12a-Based Systems For Amphibian Detection From Dietary And Environmental Dna, Macie Chess
Evaluation And Application Of Crispr-Cas12a-Based Systems For Amphibian Detection From Dietary And Environmental Dna, Macie Chess
Electronic Theses and Dissertations
Current methods for monitoring biodiversity are hindered by detection efficiencies or rely on expensive laboratory equipment. The goal of this study was to evaluate the application of a CRISPR-Cas12a-based system for the species-specific detection of amphibians from dietary and environmental DNA samples. A Cas-12a-based platform was designed and analyzed for the fluorescent detection of three salamander species; Desmognathus fuscus, D. ochropheaus, and Erycia bislineata. This system was applied to the identification of salamander DNA extracted from the fecal sacs of nestling Louisiana Waterthrush (Parkesia monticola), amplified with PCR primers targeting the CYT B mtDNA gene, and compared to DNA metabarcoding. …
Applying Conservation Genomic Techniques To Guide Management Of The Reticulated Flatwoods Salamander (Ambystoma Bishopi), Miranda Gaupp
Applying Conservation Genomic Techniques To Guide Management Of The Reticulated Flatwoods Salamander (Ambystoma Bishopi), Miranda Gaupp
Electronic Theses and Dissertations
The Reticulated flatwoods salamander (Ambystoma bishopi) is a federally endangered amphibian endemic to the longleaf-pine ecosystem of the southeastern U.S. This study used analyses of single-nucleotide polymorphism (SNP) data, collected from 2,255 unique individuals across 5 breeding seasons, spread across the known extant range of A. bishopi, to characterize the genetic diversity and demographics of populations, genetic relationships among populations, and patterns and spatial extents of gene flow, and to evaluate potential effects of management on A. bishopi’s resiliency. Population structure was strongly hierarchical, with individual breeding ponds (n = 38) acting as semi-connected subpopulations …