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Full-Text Articles in Life Sciences

Microcirculation: Electrophysiological Basis For The Response Of Endothelial Cells To Inflammatory Mediators-Bradykinin, Kai Miao Dec 1994

Microcirculation: Electrophysiological Basis For The Response Of Endothelial Cells To Inflammatory Mediators-Bradykinin, Kai Miao

Electronic Theses and Dissertations

Using conventional microelectrodes, I studied the electrical basis for determining the resting V$\sb{\rm m}$ in intact EC's from hamsters. The resting V$\sb{\rm m}$ were found to be $-$40 mV for aortic EC's and $-$43 mV for vena caval EC's. The contributions of ions to the resting V$\sb{\rm m}$ of aortic EC's were compared in terms of the transference number (t$\sb{\rm ion}$). To develop a technique for in situ monitoring changes in V$\sb{\rm m}$ of postcapillary venular EC's in the hamster mesentery, a voltage-sensitive fluorescent probe, bisoxonol, was used to load the cells and the fluorescence signals were analyzed under an …


Factors Influencing The Oxidation Of Lipoproteins And Plasma Lipids, Yanshan Ma Dec 1994

Factors Influencing The Oxidation Of Lipoproteins And Plasma Lipids, Yanshan Ma

Electronic Theses and Dissertations

The hypothesis that antioxidant vitamins (ascorbate and tocopherols) along with urate protect blood plasma lipids from oxidation was tested. Dietary fat is also an important factor influencing plasma lipid peroxidation. The purpose of this study was to investigate the role of plasma antioxidants and dietary fat on low density lipoprotein (LDL) and plasma lipid oxidation. In the first part of this study, we compared the ability of urate and ascorbate to protect human LDL from in vitro oxidation. LDL oxidation was initiated by 15 mM of a water soluble azo-initiator in the presence or absence of ascorbate or urate. The …


Role Of The Cd40-Cd40 Ligand Interaction In Cd4(+) T Cell Activation Of Monocyte Interleukin-1 Synthesis, David H. Wagner Dec 1994

Role Of The Cd40-Cd40 Ligand Interaction In Cd4(+) T Cell Activation Of Monocyte Interleukin-1 Synthesis, David H. Wagner

Electronic Theses and Dissertations

Most studies of the induction of cytokine synthesis in monocytes have used an exogenous triggering agent such as Lipolpoysaccharide (LPS). However, during nonseptic chronic inflammatory responses (e.g., rheumatoid arthritis) monocyte activation occurs as a result of T cell generated signals. This report demonstrated that plasma membranes from anti-CD3 activated peripheral CD4$\sp{+}$ T cells (Tm$\sp{\rm A}$) but not from resting CD4$\sp{+}$ cells (Tm$\sp{\rm R}$) induced monocytes to synthesize IL-1 in the absence of costimulatory cytokines. The expression kinetics of the molecule(s) unique to activated T cells which interact with monocyte receptors to induce IL-1 demonstrated that optimal expression occurred at 6h …


The Hydroclimatic Characteristics Of Nacogdoches, Texas With Implications For Water Resources Management, Larry David Clendenen Aug 1994

The Hydroclimatic Characteristics Of Nacogdoches, Texas With Implications For Water Resources Management, Larry David Clendenen

Electronic Theses and Dissertations

Sayok's (1986) study and statistical analysis of the climate at Nacogdoches, Texas covering 1901 to 1980 is updated to include the most recent twelve years (1981-92) of records. Missing data are estimated, all analyses are repeated, and the new 1961-90 normal climate is compared to the 1951-80 and other normals. Results showed that the climate at Nacogdoches during the last decade was about the same as that in other recent decades. Thomthwaite's (1957) climate classification defines Nacogdoches as moist subhumid, third mesothermal, with moderate summer water deficiency, and a temperature effIciency regime equal to a fourth mesothermal climate. There are …


Denervation Supersensitivity Of The Rat Vas Deferens: A Role For Protein Kinase C, Sonny T. Abraham May 1994

Denervation Supersensitivity Of The Rat Vas Deferens: A Role For Protein Kinase C, Sonny T. Abraham

Electronic Theses and Dissertations

A role for protein kinase C (PKC) in the denervation-induced supersensitivity of the rat vas deferens was investigated. Chronic, surgical denervation of the rat vas deferens (up to 8 days) resulted in tissues that produced enhanced contractile responses to norepinephrine (NE) in isolated organ baths. Single challenges of NE (10 $\mu$M) produced 0.6 $\pm$ 0.1 g of maximal tension in the control vas whereas in the paired, denervated tissue 2.2 $\pm$ 0.3 g of tension was recorded (n = 6). Cumulative concentration-effect curves to NE produced in the denervated vas deferens were shifted 18-fold to the left of the control …


Differential Role Of The Endothelium In Regulating Microvascular Blood Flow, Tao Tang May 1994

Differential Role Of The Endothelium In Regulating Microvascular Blood Flow, Tao Tang

Electronic Theses and Dissertations

The vascular endothelial cell (EC) plays an important role in regulating vascular tone and local blood flow by sensing chemical and mechanical stimuli on the vascular wall and releasing a host of vasoactive substances upon activations of endogenous or exogenous vasoactive substances. The central hypothesis is that local control of blood now and autoregulatory behavior in the microcirculation is distinctive at different levels of the vasculature and is dependent on the cellular activities of the EC and its interaction with the local environment. The in vivo as well as the ex vivo, flow-controlled preparations of the hamster cheek pouch were …


Characterization Of The Vasoactivity Of Tachykinins In Isolated Rat Kidney: Functional Studies And In Vitro Receptor Autoradiography, Yuejin Chen May 1994

Characterization Of The Vasoactivity Of Tachykinins In Isolated Rat Kidney: Functional Studies And In Vitro Receptor Autoradiography, Yuejin Chen

Electronic Theses and Dissertations

Although tachykinins have potent vascular actions, their effect on renal resistance blood vessels is currently unknown. The vasoactive properties of tachykinins and related analogs were assessed in isolated perfused rat kidney. At a basal perfusion pressure (PP) of 75 $\pm$ 6 mm Hg (n = 5), bolus injections of substance P (SP) had no significant vasoactive effect. Following a sustained increase in baseline PP (134 $\pm$ 10 mm Hg) produced by phenylephrine (1 $\mu$M), SP evoked a dose-dependent increase in PP. The largest dose of SP increased PP by 60 $\pm$ 5 mm Hg. The vasoconstrictor response to SP was …


An Evaluation Of A Pilot Interpretive Program For National Forest Recreation Areas In East Texas, Ronda Elain Sutphen May 1994

An Evaluation Of A Pilot Interpretive Program For National Forest Recreation Areas In East Texas, Ronda Elain Sutphen

Electronic Theses and Dissertations

A visitor survey was conducted at Ratcliff and Double Lake National Forest Recreation Areas to evaluate visitor characteristics and the effectiveness of interpretive services as a management tool. Two hundred eighty-two campers completed the visitor survey. A one hundred percent sample was attempted at both recreation areas every Sunday morning during July and August of 1991. Data from both recreation areas were analyzed and compared in order to determine any significant differences between visitors in each recreation area and also between visitors who attended or did not attend interpretive programs.


Beaver Recolonization In South-Central Maine, Laurence Lisle Ii May 1994

Beaver Recolonization In South-Central Maine, Laurence Lisle Ii

Electronic Theses and Dissertations

Following a long absence due to over-trapping, beaver (Castor canadensis) recolonized most of their continental range during the middle of the 20th century. The spread of beaver across the landscape was revealed by the emergence of beaver-created wetlands, or flowages. Based on the appearance of flowages on aerial photographs from 1939, 1957, 1974, and 1991, I documented the return of beaver to a 105 km^ watershed in south-central Maine. I sought to determine if certain wetland characteristics—area, perimeter to dam length (p/d) ratio, and watershed size—influenced the order in which sites were occupied by beaver. Also, to gauge the effect …


Characteristics Of Milk And Reduced Fat Cheddar Cheese From Cows Fed Extruded Soybeans And Niacin, Matthew Ryan Lentsch Jan 1994

Characteristics Of Milk And Reduced Fat Cheddar Cheese From Cows Fed Extruded Soybeans And Niacin, Matthew Ryan Lentsch

Electronic Theses and Dissertations

Today's consumers are demanding food products that are "low-fat", "reduced fat", "high in -unsaturated fatty acids", and "low cholesterol". This trend in food product selection is at the advice of many health professionals. Current health recommendations are to lower the dietary intake of fat, especially saturated fat. In 1992, total dairy sales accounted for over 10% of total food sales (Milk Industry Foundation, 1993). Since dairy products are perceived as being hypercholesterolemic, a market exists for dairy products which are "lower in fat" and/or "lower in saturated fat". It is said that "consumer perception is marketing reality", and because of …