Life Sciences Commons^™

Screening H3 Histone Acetylation In A Wild Bird, The House Sparrow (Passer Domesticus), Daniella Ray

Electronic Theses and Dissertations

Epigenetic mechanisms are increasingly understood to have major impacts across ecology. However, one molecular epigenetic mechanism, DNA methylation, currently dominates the literature. A second mechanism, histone modification, is likely important to ecologically relevant phenotypes and thus warrants investigation, especially because molecular interplay between methylation and histone acetylation can strongly affect gene expression. There are a limited number of histone acetylation studies on non-model organisms, yet those that exist show that it can impact gene expression and phenotypic plasticity. Wild birds provide an excellent system to investigate histone acetylation, as free-living individuals must rapidly adjust to environmental change. Here, we screen …

Exploring The Consistency Of Flow Regimes Within And Among Ecoregions Of The Southeastern United States, Frank Paul Braun Iv

Electronic Theses and Dissertations

Human manipulation of river systems has long been a known contributor to the loss of freshwater biodiversity. By accounting for environmental causes of hydrologic variation among rivers, we can better understand how ecoregion mediates flow regimes and forecast species that may be at risk. Presumably, natural variation associated with ecoregion boundaries exerts strong influence on flow regimes, and may mediate relationships between other features (e.g., land use, dam operations) and hydrology. However, such between-ecoregion variation is poorly investigated, particularly at fine spatial and temporal scales. I characterized 10 hydrologic metrics, representing the five key dimensions of the flow regime (magnitude, …

The Effects Of Cucurbitacin B And Silmitasertib On Metastasis And Itga6 Using The Zebrafish Tumor-Xenograft, Alexandra Griffis

Electronic Theses and Dissertations

Ninety percent of cancer deaths are resultant from the metastasis of cancer cells. When cancer cells translocate through blood vessels or the lymphatic system, they may form tumors outside of their primary site. The processes of metastasis can begin quickly; after onset, metastasis is unforgiving, as it does not participate with the body’s physiological systems in an orderly way. In the past, our lab produced results indicating that a cell adhesion molecule, Integrin Alpha-6, may contribute to cancer cells' ability to metastasize. Integrins mediate interactions between the cell and the Extracellular Matrix (ECM), regulating cell attachment and cell migration. With …

Dna Methylation And The Response To Infection In Introduced House Sparrows, Melanie Gibson

Electronic Theses and Dissertations

Epigenetics is the study of molecular modification of a genome without changing its base pairs. The most studied type of epigenetic mechanism is DNA methylation, which is capable of turning a gene “on” or “off.” Epigenetic potential is the capacity to which an individual can have methylation on its genome. The more CpGs available, the greater the epigenetic potential. In invasive species, genetic variation has been observed to be paradoxical: not much of it exists on a genomic level, but epigenetically, phenotypic variation can occur. The focus on shift in gene expression in this study is on Toll-Like Receptor 4 …

Sustainability Practices In Aquaculture: Using Algae Turf Scrubber Biomass To Raise Black Soldier Flies As An Alternative Feed In Blue Tilapia, Oreochromis Aureus, Culture, Michelle C. Lowery

Electronic Theses and Dissertations

Since the 1980s, non-algal aquaculture has grown to encompass 49% of all seafood production in response to a growing human population and increased seafood demand (FAO, 2022). Hurdles exist to aquaculture sustainability, including dependence on wild sourced fishmeal (FM) and the impacts wastewater discharge. It takes 4-5 tons of wild forage fish to produce one ton of dry FM (Miles and Chapman, 2006) and as aquaculture is primarily conducted in earthen ponds and public open water bodies (FAO, 2022), finfish culture can have a high impact on the surrounding environment by discharging excess nutrients. This study used algae turf scrubber …

Middle Savannah River: An A/R/Tographic Ecopedagogical Ethnography Experimenting With Rhizomatic Perspectives, Lisa Augustine-Chizmar

Electronic Theses and Dissertations

This research is an experiment in perspective. Using the four commonplaces (Schwab, 1978), I practiced letting the Savannah River teach me what there is to know about the water, the land, the people, and the other entities that depend on ki through artistic, ethnographic, and ecopedagogical lenses. The ethnographic findings describe the social actors that depend on ki and give a voice to the River. The a/r/tographic findings display the River on a canvas map through two hundred years using paint, clay, photography, video, abstract acrylics, and fabric. Together, these methods contribute to a unique ecopedagogical journey. This word cloud …

Investigating Salinity Intrusion Effects On Sapelo Island, Georgia Using Benthic Macroinvertebrate Bioindicators, Raymond Paul Kidder Ii

Electronic Theses and Dissertations

Coastal wetlands are threatened by the compounding effects of sea level rise and historical land modification. Historically, much of Sapelo Island, Georgia was deforested and drained using a series of ditches and canals to create arable land fit for agriculture and to provide drainage to the inhabited parts of the island. Today, this network of artificial channels is believed to be facilitating the movement of saltwater from the nearby estuary into freshwater areas on the interior of the island. This study aimed to better understand the frequency and magnitude of salinity intrusion events along with their resulting impacts to the …

Effects Of Occupational Tasks And Personal Protective Equipment On Static And Functional Balance Among Career Firefighters, Petra Kis

Electronic Theses and Dissertations

BACKGROUND: Musculoskeletal injuries are commonly incurred on the fireground among firefighters. Most injuries are attributable to overexertion, fall, jump, slip, and trip mechanisms. These injuries may be caused by many factors, including the independent and collective effects of wearing personal protective equipment (PPE) and the fatigue induced by performing occupational tasks. Therefore, the purpose of this study was to investigate the effect of PPE and fire suppression tasks on static and functional balance among firefighters. METHODS: 32 healthy male career structural firefighters (Age: 31.8±9.5 yr) volunteered for this study. Static and Functional Balance Tests (FBT) were performed in …

The Relationship Between Chronotypes And Cardiovascular Disease Risk Factors Among Law Enforcement Officers, Alyssa N. Fail

Electronic Theses and Dissertations

Much of the data associated with cardiovascular disease risk factors and circadian rhythms are done on shift workers; due to their line of work typically causing circadian disruption and sleep loss (Loef et al., 2019). Previous research incorporating chronotype and its influence on shift work has been explored; however, there is limited research focusing on law enforcement officers. Additionally, no known research has investigated the effect of chronotype on CVD risk factors among law enforcement officers. The aim of this study was to investigate the cardiovascular disease risk factors in law enforcement officers and their relationship with chronotypes. This cross-sectional …

Evaluating Blood Pressure And Hypertension Awareness In Municipal Workers In The Southeast Us, Kayden R. Mcalvin

Electronic Theses and Dissertations

The primary purpose of this study was to describe municipal workers’ blood pressure (BP) status and awareness; the secondary purpose was to examine the association between BP misconception and department. Municipal employees in a southeastern rural community (n=75) were asked to participate in a 15-minute survey, including the BASIS Hypertension Awareness survey, and blood pressure measurements to determine hypertension risk. Results show a significant difference in diastolic blood pressure (DBP) between police with a higher DBP of 87.64 ± 10.9 mm Hg and fire departments 78.98 ± 11.1 mm Hg (p=0.005), a significant difference in DBP between city hall/public works …

Endogenous Antioxidant Response And Lipid Peroxidation Levels In Muscle Tissue Of The Cownose Ray (Rhinoptera Bonasus), Bailey Berry

Electronic Theses and Dissertations

Oxidative stress is the result of an imbalance between reactive oxygen species (ROS) and antioxidant response within cells. ROS generation is a natural consequence of ETC activity within the mitochondria and is associated with aerobic metabolism. Oxidative stress data on elasmobranch muscle is limited, with previous studies primarily focusing on mammalian and teleost muscle. Elasmobranch skeletal muscle is generally composed of large proportions of white muscle fibers (anaerobic), low proportions of red muscle fibers (aerobic), and low proportions of pink muscle fibers (anaerobic). The cownose ray, a benthopelagic batoid species, presents an interesting model of study because it contains relatively …

The Effect Of Genetic Relatedness On Mate Selection And Spatial Distribution In The American Alligator, Alligator Mississippiensis, Mark Hoog

Electronic Theses and Dissertations

To date, most genetic studies on the American alligator (Alligator mississippiensis) have focused on population genetics and multiple paternity. There have not been any studies looking at how relatedness affects alligator behavior and movement. This study focused on three main questions: 1) what is the genetic diversity among alligators in this location? 2) can parentage be accurately defined among parents and offspring? and 3) how does individual relatedness affect their spatial distribution? We examined the relatedness of 174 unique individuals from the Okefenokee Swamp Park, in South Georgia, USA. In addition, we placed Telonics^® satellite tags on …

Use Of Hematological Markers To Assess Physiological Condition And Health Status In Free-Ranging Sand Tiger Sharks (Carcharius Taurus), Chestina N. Craig

Electronic Theses and Dissertations

The contents of blood can provide information about the physiological condition and health of vertebrates. This study seeks to better understand the stress physiology and blood bacteria presence of the sand tiger shark (Carcharius taurus), as sharks are known to have unique physiology and immune systems. In this study the blood metabolites glucose, lactate, and ketones (3-hydroxybuteric acid and acetoacetate), were used to understand how biotic and abiotic factors affect the acute stress response to capture and handling. Metabolite concentrations from blood plasma were analyzed using colorimetric assays. Glucose and ketones showed no significant responses to capture and …

Spatial Drivers Of Freshwater Fish Biodiversity, Community Structure, And Diet Composition Across Georgia, Usa, Patrick Lewis

Electronic Theses and Dissertations

The geographic complexity of the Southeastern United States, in particular Georgia, is evident in the diversity of stream habitats present throughout that host unique stream fish communities but is threatened by anthropogenic activity. This spatial complexity can be captured through the ecoregion framework, which delineates areas of distinct habitat. However, this differing habitat character between ecoregions leads to different environmental pressures that may promote species heterogeneity between communities, may create intraspecific niche diversity in widely distributed species, and may complicate the transferability of widely accepted theoretical models of fish ecology between them. I analyzed a long-term fish community dataset, examining …

Hepatic Transcriptomic Assessment Of Sprague Dawley Rats In Response To Dietary Perfluorobutane Sulfonate (Pfbs) Ingestion, Isaac Appiah

Electronic Theses and Dissertations

Perfluorobutane sulfonate (PFBS) is a short-chain PFAS that is considered to be a less toxic replacement for the rather more toxic long-chain perfluorooctane sulfonate (PFOS). Its numerous industrial applications and widespread presence in the environment have raised environmental and health concerns because of growing evidence associating adverse health effects and certain liver diseases to PFBS exposure. The study goal was to investigate whether dietary ingestion of PFBS would induce liver injuries, damage, inflammations, and oxidative stress. To achieve these goals, Sprague-Dawley rats were assigned into three PFBS dietary treatment groups (0, 50, and 100 PPM) for 11 weeks. After this …

Developing Best Practices For The Propagation Of Spartina Alterniflora For Use In Salt Marsh Restortaion, Justin Hinson

Electronic Theses and Dissertations

Coastal salt marshes are valuable ecosystems under threat from climate change and sea level rise. Living shorelines offer a promising solution, often incorporating the foundational salt marsh species Spartina alterniflora due to its ability to tolerate natural stressors and maintain sediment stability. However, research suggests that seed-based propagation protocols should be developed on a local scale due to the genetic heterogeneity within and between S. alterniflora populations. Here, we attempt to contribute to the development of one such protocol for coastal Georgia S. alterniflora.

In Fall 2021, seeds were collected bi-monthly from four marshes of varying ocean proximity and …

The Effect Of Weekly Training Load On Match Result In American Professional Soccer, Cameron M. Horsfall

Electronic Theses and Dissertations

BACKGROUND: During a professional soccer season, training load (TL) will fluctuate due to the need to prepare for the next competition. Based on the technical staff’s daily and weekly evaluation, TL will be adjusted as they prepare the team and maximize individual performance and achieve a favorable result. Therefore, the programming of each match week (MW) TL may promote a favorable result. PURPOSE: The purpose of this study was to examine variation in daily GPS-derived TL variables based on match result using the Match Day Minus (MD-) system. This study provides descriptive TL data yet to be reported in …

Comparative Analytics On Chilli Plant Disease Using Machine Learning Techniques, Sai Abhishta Roy Seelam

Electronic Theses and Dissertations

This thesis concerns the detection of diseases in chilli plants using machine learning techniques. Three algorithms, viz., Convolutional Neural Network (CNN), Recurrent Neural Network (RNN), and Multi-Layer Perceptron (MLP), and their variants have been employed. Chilli-producing countries, India, Mexico, China, Indonesia, Spain, the United States, and Turkey. India has the world’s largest chilli production of about 49% (according to 2020). Andhra Pradesh (Guntur) is the largest market in India, where their varieties are more popular for pungency and color. This study classifies five kinds of diseases that affect the chilli, namely, leaf spot, whitefly, yellowish, healthy, and leaf curl. A …

The Effect Of Tempo On Prone Hamstring Exercise - An Exploration Of Unilateral Bias, Derick A. Anglin

Electronic Theses and Dissertations

Hamstring injuries are common muscle-skeletal injuries with a high recurrence rate. While unmodifiable risk factors (e.g., previous injury) vary, recent evidence has questioned the utility of eccentric exercise in preventing hamstring strains. Furthermore, whether eccentric exercise can transfer to sprinting’s kinetic and kinematic requirements is of question. Therefore, this investigation examines exercise tempo (fast vs. slow eccentric phase), movement strategy (bilateral vs. unilateral), and external load characteristics (coupled vs. independent) bias concentric (CON) and eccentric (ECC) biceps femoris (BF) mean electromyography (mEMG). METHODS: Twenty college-aged students aged 18 – 33 (M = 23.4, SD = 3.12 years) with …

Effect Of Training Modality On Intention To Exercise Among Law Enforcement Cadets Post Academy, Nicholas Hunt

Electronic Theses and Dissertations

Police academies are typically the first formalized job-task-oriented exercise training for law enforcement officers (LEOs). Research has demonstrated that cadets are at higher risk of injury during the academy. Physical training (PT) is a critical component of LEO academy efforts to build occupational skills to meet job demands. Although research is beginning to investigate the efficacy of different training modalities, more research is needed to understand the impact of academy training load and modality on occupational readiness and interest in maintaining exercise behaviors post-academy. PURPOSE: to investigate the relationships between different physical training modalities and intention to continue physical training …

Remote Sensing Of Georgia Tidal Marsh Habitats Using Aerial Photography And Planetscope Satellite Imagery, Harrison M. Currin

Electronic Theses and Dissertations

Globally, tidal marshes cover about 90,800 km. Within the state of Georgia tidal marshes are primarily located behind the barrier islands and total 1,619 km². The combination of high salinity environments and daily inundation, and being dependent on river output, make these dynamic systems. Tidal marshes provide numerous ecosystem services such as carbon and nitrogen sequestration, flood control, coastal protection, and numerous biogeochemical processes. Due to their unique position, tidal marshes are under threat from sea level rise, drought, coastal development, and large-scale disturbance events. Tidal freshwater marshes are especially susceptible to these threats due to their geographic …

Mortality In Interspecific Hybrids Of Nasonia Vitripennis And Nasonia Giraulti, Bonnie Cobb

Electronic Theses and Dissertations

Nasonia is a parasitoid wasp genus that serves as an emerging model for studying speciation due to an incompatibility between nuclear and mitochondrial genomes between sister taxa. Short generation times, easy rearing in a lab setting, producing large amounts of progeny, and whole genome sequencing make Nasonia is an excellent candidate for studying incomplete reproductive isolation. Nasonia have five chromosomes and exhibit haplo-diploid sex determination in which fertilized eggs develop into diploid females and unfertilized eggs develop into haploid males. Recessive phenotypes are hidden in diploid females but are laid bare in haploid males as there is no interaction between …

The Development Of A Novel Throwing Assessment For Children With Cerebral Palsy, Cory Cribb

Electronic Theses and Dissertations

Introduction: Cerebral palsy is one of childhood's most common developmental disabilities. Cerebral palsy leads to dysfunction in the upper extremities due to symptoms such as low muscle power, spasticity, and increased tone. A modified throwing assessment was constructed to examine throwing performance as a measure of upper extremity function. Purpose: To determine if the modified throwing assessment is a reliable measure of throwing performance and if the modified throwing assessment is a valid measure of upper extremity power in children with CP. Methods: The study employed a test-retest design aimed at determining the reliability of a modified throwing assessment. Seven …

Applying Conservation Genomic Techniques To Guide Management Of The Reticulated Flatwoods Salamander (Ambystoma Bishopi), Miranda Gaupp

Electronic Theses and Dissertations

The Reticulated flatwoods salamander (Ambystoma bishopi) is a federally endangered amphibian endemic to the longleaf-pine ecosystem of the southeastern U.S. This study used analyses of single-nucleotide polymorphism (SNP) data, collected from 2,255 unique individuals across 5 breeding seasons, spread across the known extant range of A. bishopi, to characterize the genetic diversity and demographics of populations, genetic relationships among populations, and patterns and spatial extents of gene flow, and to evaluate potential effects of management on A. bishopi’s resiliency. Population structure was strongly hierarchical, with individual breeding ponds (n = 38) acting as semi-connected subpopulations …

The Impact Of Gloves And Simulated Occupational Tasks On Handgrip Strength In Structural Firefighters, Sarah Lanham

Electronic Theses and Dissertations

Adequate handgrip strength (HGS) is important to safely and effectively perform fireground tasks. However, there is limited research describing the deleterious impact of glove use and fatigue from occupational tasks on HGS. Therefore, the aims of this investigation were to quantify the impact of glove use and occupational tasks on HGS, to explore the relationship between HGS versus the glove and task-induced decrement in HGS, and to evaluate the relationship between HGS and decrement in HGS versus occupational performance. Fourteen (Males: n=13) career structural firefighters performed a maximal isometric HGS assessment with and without gloves before and immediately following completion …

Development Of Novel Pyridazine Derivatives And Drug Delivery Systems Against Dengue, Janae A. Culmer

Electronic Theses and Dissertations

The lack of approved vaccines, medications and treatment regimens has significantly contributed to the rapid spread of mosquito-borne viruses such as Dengue and Zika virus. The complex immunopathology of these viruses presents limitations for the development and implementation of a definitive, safe and effective approach to combat infections.Previous research has demonstrated that vector control strategies such as the elimination of larval habitats, larviciding with insecticides, the use of biological agents and the application of adulticides have been unsuccessful in the reduction of viral transmission leading to the need for the continued development of antivirals. This research proposes an approach for …

Biomimetic Synthesis Of Palladium Nanoparticles For Catalytic Application, Emily A. Groover

Electronic Theses and Dissertations

The synthesis of palladium nanoparticles (Pd NPs) using materials-directed peptides is a novel, nontoxic approach which exerts a high level of control over the particle size and shape. This biomimetic technique is environmentally benign, featuring nonhazardous ligands and ambient conditions. Nanoparticles are extremely reactive catalysts, boasting a large surface-to-volume ratio when compared to their bulk counterparts. The rational design of these nanoparticles using peptides has been very successful in aqueous environments, but no research has been done to apply it in organic systems. As such, the biomimetic synthesis of Pd NPs in an organic system is here investigated, with ethanol …

Stress, Stress Appraisal And Coping In Athletic Trainers During The Covid-19 Pandemic, Lawson Holton

Electronic Theses and Dissertations

Introduction: There have been many studies conducted on the stress and mental health of frontline healthcare workers (HCWs) over the past months since the COVID-19 pandemic began. Very limited literature has examined the effect of the pandemic on athletic trainers (ATs). Objective: The aim of this investigation was to examine the stress, stress appraisal, and coping measures of ATs during the COVID-19 pandemic. Methods: Participants were recruited members from the National Athletic Trainers’ Association (NATA). The Perceived Stress Scale-10 was used to measure stress, the Stress Appraisal Measure was used to measure stress appraisal, and the Brief COPE was used …

Evaluation Of Hemp Seed Meal As A Fish Meal Replacement Through Growth And Digestibility Trials In Striped Bass (Morone Saxatilis), Anthony Sample

Electronic Theses and Dissertations

Demand for seafood has continued to increase despite a stagnation in global wild-caught fisheries. Aquaculture has met this demand but suffers from numerous sustainability issues because of dependence on fish meal, which is unsustainable, sparking nutritional research into alternatives. Hemp seed meal has shown promise as an alternative due to its sustainable production and nutritional qualities. This study evaluated hemp seed meal as an alternative to fish meal through growth and digestibility trials with Morone saxatilis. A seven-week growth trial was conducted within a recirculating aquaculture system using a total of two control diets (commercial and constructed) and six …

The Influence Of Faculty Peer Network Communication In The Diffusion Of A Centralized Cure, Nicole L. Scheuermann

Electronic Theses and Dissertations

Course-based undergraduate research experiences (CUREs) provide students the benefits of undergraduate research participation by incorporating authentic scientific research into laboratory courses. CUREs are a relatively young pedagogy and are therefore innovative. Roger’s (2003) diffusion of innovations (DOI) framework posits that the diffusion of innovations, such as CUREs, is a highly social process. Most existing CURE research has focused on the impacts to students and the critical elements of CURE design. Investigation into instructor peer network communication is largely absent from the existing CURE literature. This study investigates the structure and function of a CURE community – the Malate Dehydrogenase CURE …