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Use Of Two-Dimensional Agarose-Gel Analysis To Characterize Processing Of Uv-Irradiated Plasmids And The Composition Of The Replisome Following Uv-Induced Arrest, Harout Arthur Jeiranian
Use Of Two-Dimensional Agarose-Gel Analysis To Characterize Processing Of Uv-Irradiated Plasmids And The Composition Of The Replisome Following Uv-Induced Arrest, Harout Arthur Jeiranian
Dissertations and Theses
In this thesis, I address two fundamental questions related to our understanding of how DNA damage is processed and repaired during replication. Using Two-dimensional (2-D) agarose gel analysis, I first examine whether DNA damage on plasmids introduced by transformation is processed in a manner similar to that observed on endogenously replicating plasmids and the chromosome. The original intent for using this approach was to develop a technique that could examine how different DNA adducts would be repaired in various sequence contexts. However, I found that distinct differences exist between the processing of DNA damage on transforming plasmids and the chromosome. …