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Full-Text Articles in Life Sciences
Inheritance Of Cry1f Resistance, Crossresistance And Frequency Of Resistant Alleles In Spodoptera Frugiperda (Lepidoptera: Noctuidae), A. M. Vélez, T. A. Spencer, A. P. Alves, D. Moellenbeck, R. L. Meagher, H. Chirakkal, B. D. Siegfried
Inheritance Of Cry1f Resistance, Crossresistance And Frequency Of Resistant Alleles In Spodoptera Frugiperda (Lepidoptera: Noctuidae), A. M. Vélez, T. A. Spencer, A. P. Alves, D. Moellenbeck, R. L. Meagher, H. Chirakkal, B. D. Siegfried
Department of Entomology: Faculty Publications
Transgenic maize, Zea maize L., expressing the Cry1F protein from Bacillus thuringiensis has been registered for Spodoptera frugiperda ( J. E. Smith) control since 2003. Unexpected damage to Cry1F maize was reported in 2006 in Puerto Rico and Cry1F resistance in S. frugiperda was documented. The inheritance of Cry1F resistance was characterized in a S. frugiperda resistant strain originating from Puerto Rico, which displayed >289-fold resistance to purified Cry1F. Concentration–response bioassays of reciprocal crosses of resistant and susceptible parental populations indicated that resistance is recessive and autosomal. Bioassays of the backcross of the F1 generation crossed with the resistant parental …
On-Plant Survival And Inheritance Of Resistance To Cry1ab Toxin From Bacillus Thuringiensis In A Field-Derived Strain Of European Corn Borer, Ostrinia Nubilalis, Andre L. B. Crespo, Terence A. Spencer, Analiza P. Alves, Richard L. Hellmich, Erin E. Blankenship, Leonardo C. Magalhães, Blair D. Siegfried
On-Plant Survival And Inheritance Of Resistance To Cry1ab Toxin From Bacillus Thuringiensis In A Field-Derived Strain Of European Corn Borer, Ostrinia Nubilalis, Andre L. B. Crespo, Terence A. Spencer, Analiza P. Alves, Richard L. Hellmich, Erin E. Blankenship, Leonardo C. Magalhães, Blair D. Siegfried
Department of Entomology: Faculty Publications
BACKGROUND: The high dose plus refuge is one of the major components of the resistance management plan mandated for transgenic corn expressing Cry toxins from Bacillus thuringiensis Berliner (Bt) that targets the European corn borer, Ostrinia nubilalis (Hübner) (Lepidoptera: Crambidae). This strategy was based on assumptions such as functional recessive inheritance, which has not been previously tested for O. nubilalis. The authors used a field-derived resistant strain of O. nubilalis to define the nature of resistance to Cry1Ab toxin by examining the inheritance and on-plant survival of susceptible and resistant insects and their F1 progeny.
RESULTS: The …
Utility Of Morphological And Molecular Techniques For Determination Of Paternity In Two Subspecies Of Diabrotica Undecimpunctata (Coleoptera: Chrysomelidae), Isaac O. Oyediran, Thomas L. Clark, Steven R. Skoda, Elvis A. Heinrichs
Utility Of Morphological And Molecular Techniques For Determination Of Paternity In Two Subspecies Of Diabrotica Undecimpunctata (Coleoptera: Chrysomelidae), Isaac O. Oyediran, Thomas L. Clark, Steven R. Skoda, Elvis A. Heinrichs
Department of Entomology: Faculty Publications
An experiment was conducted to determine the paternity of F 1 progeny using morphological and molecular methods in Diabrotica (Coleoptera: Chrysomelidae) subspecies: Diabrotica undecimpunctata howardi Barber, also known as spotted cucumber beetle, and D. undecimpunctata undecimpunctata Mannerheim, also known as western cucumber spotted beetle. Results from crosses that involved the females of D. u. howardi and the males of D. u. undecimpunctata had all F, progeny with phenotypes as the male parent. Similarly, in all the crosses that involved the females of D. u. undecimpunctata and the males of the D. u. howardi, all the F1 progeny had …
Inheritance Of Resistance To The Cry1ab Bacillus Thuringiensis Toxin In Ostrinia Nubilalis (Lepidoptera: Crambidae), Analiza P. Alves, Terrence A. Spencer, B.E. Tabashnik, Blair Siegfried
Inheritance Of Resistance To The Cry1ab Bacillus Thuringiensis Toxin In Ostrinia Nubilalis (Lepidoptera: Crambidae), Analiza P. Alves, Terrence A. Spencer, B.E. Tabashnik, Blair Siegfried
Department of Entomology: Faculty Publications
Laboratory selection with Cry1Ab, the predominant Bacillus thuringiensis (Bt) toxin in transgenic corn, Zea mays L., produced >1000-fold resistance in two laboratory strains of European corn borer, Ostrinia nubilalis (Hubner). We tested the offspring of various crosses to determine the mode of inheritance of resistance to Cry1Ab. Patterns of inheritance of resistance were similar in the two resistant strains. The progeny of reciprocal F1 crosses (resistant male x susceptible female and vice versa) responded alike in bioassays, indicating autosomal inheritance. The median lethal concentrations (LC50 values) of F1 were intermediate between the resistant and susceptible parents, indicating …
Inheritance Of Methyl-Parathion Resistance In Nebraska Western Corn Rootworm Populations (Coleoptera: Chrysomelidae), Srivinas Parimi, Michael E. Scharf, Lance J. Meinke, Laurence D. Chandler, Blair D. Siegfried
Inheritance Of Methyl-Parathion Resistance In Nebraska Western Corn Rootworm Populations (Coleoptera: Chrysomelidae), Srivinas Parimi, Michael E. Scharf, Lance J. Meinke, Laurence D. Chandler, Blair D. Siegfried
Department of Entomology: Faculty Publications
Field populations of western corn rootworm, Diabrotica virgifera virgifera LeConte, were collected from three different sites (York Co., Phelps Co., and Saunders Co.) in Nebraska during 1996.Adult bioassays of these three populations were conducted with different concentrations of methyl-parathion and at a diagnostic concentration (1.0 μg/ml) to determine resistance levels among these populations. Self and reciprocal crosses were made between the two resistant and one susceptible laboratory-reared populations. Dose-responses and dominance ratios calculated for the four reciprocal crosses indicated that resistance was incompletely dominant in both strains, although in one of the strains there was an indication of sex linkage. …