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Full-Text Articles in Life Sciences
Microbiome Variation Across Populations Of Desert Halophyte Zygophyllum Qatarensis, Abdul Latif Khan, Lucas Dantas Lopes, Saqib Bilal, Sajjad Asaf, Kerri M. Crawford, Venkatesh Balan, Ahmed Al-Rawahi, Ahmed Al-Harrasi, Daniel P. Schachtman
Microbiome Variation Across Populations Of Desert Halophyte Zygophyllum Qatarensis, Abdul Latif Khan, Lucas Dantas Lopes, Saqib Bilal, Sajjad Asaf, Kerri M. Crawford, Venkatesh Balan, Ahmed Al-Rawahi, Ahmed Al-Harrasi, Daniel P. Schachtman
Department of Agronomy and Horticulture: Faculty Publications
Microbial symbionts play a significant role in plant health and stress tolerance. However, few studies exist that address rare species of core-microbiome function during abiotic stress. In the current study, we compared the microbiome composition of succulent dwarf shrub halophyte Zygophyllum qatarensis Hadidi across desert populations. The results showed that rhizospheric and endosphere microbiome greatly varied due to soil texture (sandy and gravel). No specific bacterial amplicon sequence variants were observed in the core-microbiome of bulk soil and rhizosphere, however, bacterial genus Alcaligenes and fungal genus Acidea were abundantly distributed across root and shoot endospheres. We also analyzed major nutrients …
Microbiome Variation Across Populations Of Desert Halophyte Zygophyllum Qatarensis, Abdul Latif Khan, Lucas Dantas Lopes, Saqib Bilal, Sajjad Asaf, Kerri M. Crawford, Venkatesh Balan, Ahmed Al-Rawahi, Ahmed Al-Harrasi, Daniel P. Schachtman
Microbiome Variation Across Populations Of Desert Halophyte Zygophyllum Qatarensis, Abdul Latif Khan, Lucas Dantas Lopes, Saqib Bilal, Sajjad Asaf, Kerri M. Crawford, Venkatesh Balan, Ahmed Al-Rawahi, Ahmed Al-Harrasi, Daniel P. Schachtman
Department of Agronomy and Horticulture: Faculty Publications
Microbial symbionts play a significant role in plant health and stress tolerance. However, few studies exist that address rare species of core-microbiome function during abiotic stress. In the current study, we compared the microbiome composition of succulent dwarf shrub halophyte Zygophyllum qatarensis Hadidi across desert populations. The results showed that rhizospheric and endosphere microbiome greatly varied due to soil texture (sandy and gravel). No specific bacterial amplicon sequence variants were observed in the core-microbiome of bulk soil and rhizosphere, however, bacterial genus Alcaligenes and fungal genus Acidea were abundantly distributed across root and shoot endospheres. We also analyzed major nutrients …
Rhizosphere Microbiomes In A Historical Maize-Soybean Rotation System Respond To Host Species And Nitrogen Fertilization At The Genus And Subgenus Levels, Michael A. Meier, Martha G. Lopez-Guerrero, Ming Guo, Marty Schmer, Josh Herr, James Schnable, James R. Alfano, Jinliang Yang
Rhizosphere Microbiomes In A Historical Maize-Soybean Rotation System Respond To Host Species And Nitrogen Fertilization At The Genus And Subgenus Levels, Michael A. Meier, Martha G. Lopez-Guerrero, Ming Guo, Marty Schmer, Josh Herr, James Schnable, James R. Alfano, Jinliang Yang
Department of Agronomy and Horticulture: Faculty Publications
Root-associated microbes are key players in plant health, disease resistance, and nitrogen (N) use efficiency. It remains largely unclear how the interplay of biological and environmental factors affects rhizobiome dynamics in agricultural systems. In this study, we quantified the composition of rhizosphere and bulk soil microbial communities associated with maize (Zea mays L.) and soybean (Glycine max L.) in a long-term crop rotation study under conventional fertilization and low-N regimes. Over two growing seasons, we evaluated the effects of environmental conditions and several treatment factors on the abundance of rhizosphere- and soil-colonizing microbial taxa. Time of sampling, host plant species, …
Microbial Community Field Surveys Reveal Abundant Pseudomonas Population In Sorghum Rhizosphere Composed Of Many Closely Related Phylotypes, Dawn Chiniquy, Elle M. Barnes, Jinglie Zhou, Kyle Hartman, Xiaohui Li, Amy Sheflin, Ellen Marsh, Jessica Prenni, Adam M. Deutschbauer, Daniel P. Schachtman, Susannah G. Tringe
Microbial Community Field Surveys Reveal Abundant Pseudomonas Population In Sorghum Rhizosphere Composed Of Many Closely Related Phylotypes, Dawn Chiniquy, Elle M. Barnes, Jinglie Zhou, Kyle Hartman, Xiaohui Li, Amy Sheflin, Ellen Marsh, Jessica Prenni, Adam M. Deutschbauer, Daniel P. Schachtman, Susannah G. Tringe
Department of Agronomy and Horticulture: Faculty Publications
While the root-associated microbiome is typically less diverse than the surrounding soil due to both plant selection and microbial competition for plant derived resources, it typically retains considerable complexity, harboring many hundreds of distinct bacterial species. Here, we report a time-dependent deviation from this trend in the rhizospheres of field grown sorghum. In this study, 16S rRNA amplicon sequencing was used to determine the impact of nitrogen fertilization on the development of the root-associated microbiomes of 10 sorghum genotypes grown in eastern Nebraska. We observed that early rhizosphere samples exhibit a significant reduction in overall diversity due to a high …
Rhizosphere Microbiome Of Arid Land Medicinal Plants And Extra Cellular Enzymes Contribute To Their Abundance, Abdul Latif Khan, Sajjad Asaf, Raeid M. M. Abed, Yen Ning Chai, Ahmed N. Al-Rawahi, Tupan Kumar Mohanta, Ahmed Al-Rawahi, Daniel P. Schachtman, Ahmed Al-Harrasi
Rhizosphere Microbiome Of Arid Land Medicinal Plants And Extra Cellular Enzymes Contribute To Their Abundance, Abdul Latif Khan, Sajjad Asaf, Raeid M. M. Abed, Yen Ning Chai, Ahmed N. Al-Rawahi, Tupan Kumar Mohanta, Ahmed Al-Rawahi, Daniel P. Schachtman, Ahmed Al-Harrasi
Department of Agronomy and Horticulture: Faculty Publications
Revealing the unexplored rhizosphere microbiome of plants in arid environments can help in understanding their interactions between microbial communities and plants during harsh growth conditions. Here, we report the first investigation of rhizospheric fungal and bacterial communities of Adenium obesum, Aloe dhufarensis and Cleome austroarabica using next-generation sequencing approaches. A. obesum and A. dhufarensis grows in dry tropical and C. austroarabica in arid conditions of Arabian Peninsula. The results indicated the presence of 121 fungal and 3662 bacterial operational taxonomic units (OTUs) whilst microbial diversity was significantly high in the rhizosphere of A. obesum and A. dhufarensis and low …
Isolation And Analysis Of Microbial Communities In Soil, Rhizosphere, And Roots In Perennial Grass Experiments, Morgan R. Mcpherson, Peng Wang, Robert B. Mitchell, Daniel P. Schachtman
Isolation And Analysis Of Microbial Communities In Soil, Rhizosphere, And Roots In Perennial Grass Experiments, Morgan R. Mcpherson, Peng Wang, Robert B. Mitchell, Daniel P. Schachtman
Department of Agronomy and Horticulture: Faculty Publications
Plant and soil microbiome studies are becoming increasingly important for understanding the roles microorganisms play in agricultural productivity. The purpose of this manuscript is to provide detail on how to rapidly sample soil, rhizosphere, and endosphere of replicated field trials and analyze changes that may occur in the microbial communities due to sample type, treatment, and plant genotype. The experiment used to demonstrate these methods consists of replicated field plots containing two, pure, warm-season grasses (Panicum virgatum and Andropogon gerardii) and a low-diversity grass mixture (A. gerardii, Sorghastrum nutans, and Bouteloua curtipendula). Briefly, plants …