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Paramecium Bursaria Chlorella Virus 1 Proteome Reveals Novel Architectural And Regulatory Features Of A Giant Virus, David Dunigan, Ronald Cerny, Andrew T. Bauman, Jared C. Roach, Leslie C. Lane, Irina V. Agarkova, Kurt William Wulser, Giane M. Yanai-Balser, James R. Gurnon, Jason C. Vitek, Bernard J. Kronschnabel, Adrien Jeannard, Guillaume Blanc, Chris Upton, Gary Duncan, O. William Mcclung, Fangrui Ma, James L. Van Etten
Paramecium Bursaria Chlorella Virus 1 Proteome Reveals Novel Architectural And Regulatory Features Of A Giant Virus, David Dunigan, Ronald Cerny, Andrew T. Bauman, Jared C. Roach, Leslie C. Lane, Irina V. Agarkova, Kurt William Wulser, Giane M. Yanai-Balser, James R. Gurnon, Jason C. Vitek, Bernard J. Kronschnabel, Adrien Jeannard, Guillaume Blanc, Chris Upton, Gary Duncan, O. William Mcclung, Fangrui Ma, James L. Van Etten
David D Dunigan Ph. D.
The 331 kilobase pairs chlorovirus PBCV-1 genome was re-sequenced and annotated to correct errors in the original 15 year old sequence; forty codons was considered the minimum protein size of an open reading frame. PBCV-1 encodes 416 predicted protein encoding sequences and 11 tRNAs. A proteome analysis was also conducted on highly purified PBCV-1 virions using two mass-spectrometry based protocols. The mass spectrometry-derived data were compared to PBCV-1 and its host Chlorella variabilis NC64A predicted proteomes. Combined, these analyses revealed 148 unique virus-encoded proteins associated with the virion (about 35% of the coding capacity of the virus) and one host …