Life Sciences Commons^™

Gather Round The Tree: Woody Aboveground Biomass Increases Animal Presence And Species Richness In A Tropical Forest-Savanna Ecotone, Merry Vanessa Marshall Davidson

Boise State University Theses and Dissertations

Boundaries between ecosystems are often biodiversity hotspots with relatively high vulnerability to global change. The boundary between tropical rainforest and savanna ecosystems in the Amazon presents an ecotone that is undergoing a shift in ecosystem structure, as a warming climate promotes the expansion of grassland. How animal communities in the Amazon will respond to changes in ecosystem structure is a crucial unanswered question with implications for the many ecosystem services that animals provide, from a food source for Indigenous people to seed dispersal for vulnerable tree populations. Recent modeling work has forecasted that faunal savannization will occur in the Amazon, …

Effects Of Habitat Disturbances On Reptiles In Sagebrush Steppe, Kristina Joan Parker

Boise State University Theses and Dissertations

Reptiles inhabiting shrub-steppe ecosystems of the Intermountain West have adapted to harsh, unpredictable desert conditions, yet recent changes in disturbance regimes may put species at risk. In southwest Idaho, cheatgrass (Bromus tectorum) has altered the fire regime resulting in a vast conversion of shrub-steppe to mostly annual grasslands that burn too frequently to allow shrublands to recover. Southwest Idaho has the highest reptile diversity in the Pacific Northwest, yet we know little about reptile community dynamics in response to the cheatgrass-fire cycle. We hypothesized that wildfires and cheatgrass negatively affect reptile communities directly (i.e., mortality during fires) and …

Mechanisms For Extracellular Matrix-Dependent Blood-Brain Barrier Dysfunction, Brian Adam Hoettels

Boise State University Theses and Dissertations

Dysfunction of the blood vessels that form the blood-brain barrier (BBB) is observed across various neurological disorders, including multiple sclerosis (MS). As barrier loss culminates in neuronal dysfunction and degeneration, a better understanding of the mechanisms underlying BBB dysfunction is needed.

Tight junctions are multiprotein complexes maintained by the endothelial cells lining the inner blood vessel wall to seal the intercellular space, and their disruption impairs BBB function. In my first chapter, I focus on how tight junctions are altered in CNS inflammatory demyelinating diseases (CNS-IDDs) like MS as BBB dysfunction is one of the earliest known stages in …

Liposomes: Production And Applications For Controlled Drug Delivery, Gamid Abatchev

Boise State University Theses and Dissertations

This dissertation recognizes the enormous potential presented by the ever-evolving development of liposomes as drug carriers and seeks to offer further investigation into their useful production and utilization. The first chapter presents the basic principles governing their formation by self-assembly in water solutions, briefly describes the most common production methods, and points out essential past advances that led to their use as drug carriers. Chapter two exemplifies production of liposomes by the traditional methods of extrusion and sonication, detailing passive and active loading, as well as physical characterization by Dynamic Light Scattering, microscopy imaging, and fluorescence spectroscopy. In the next …

Osmr Signaling Induces Tamp-Specific Bbb Dysfunction Following Autoimmune-Mediated Neutrophil Recruitment, Travis Scott Wertz

Boise State University Theses and Dissertations

Integrity of the brain microvessels that form the blood-brain barrier (BBB) is maintained through fine-tuned regulation of endothelial tight junction proteins, chiefly represented by claudin 5 and the tight junction-associated MARVEL protein (TAMP) occludin. Under proinflammatory conditions in autoimmune-mediated multiple sclerosis (MS), autoreactive effector T cells are activated in white matter regions of the brain and spinal cord and release cytokines to recruit circulating neutrophils that, in turn, release neurotoxic substances. Lack of tight junctions in peripheral blood vessels allows neutrophils to exit blood vessels unhindered, but BBB tight junctions must first be downregulated before neutrophils can transmigrate into the …

From Microbes To Management: Spatial And Temporal Variations In Available Vegetation Affect The Gut Microbiota In A Population Of Free-Ranging Generalist Herbivores, Olivia K. Rodríguez

Boise State University Theses and Dissertations

The effects of extrinsic environmental factors that shape ecological systems are not only seen at the macroscopic level, but additionally influence and govern the host-associated microbial communities of their mammalian hosts. These microbial communities are susceptible to the fluctuation of abiotic and biotic factors which affect their host organisms. The surge in the research of microbiota–communities of archaea, bacteria, fungi, and viruses residing in various environmental systems–has shown that these communities can profoundly influence animal health. As such, monitoring microbiota has allowed for a new approach to study animal health and physiology. This is of particular benefit in the conservation …

Role Of The Nuclear Envelope In The Mechanoregulation Adipogenesis, Matthew H. Goelzer

Boise State University Theses and Dissertations

Mechanical signals are known regulators of mesenchymal stem cell (MSC) fate, regulating their differentiation into osteoblasts, chondrocytes, and adipocytes. These relevant mechanical signals reach to nucleus through nuclear envelope proteins such as Lamin A/C and the Linker of the Nucleoskeleton and Cytoskeleton (LINC) complexes. Within the context of bone, clinically relevant mutations of Lamin A/C and the LINC complexes have been shown to alter adipogenic and osteogenic MSC differentiation patterns, suggesting that that nucleo-cytoskeletal connectivity provided by nuclear envelope is important in regulating MSC fate. Using MSC adipogenesis as a model of MSC mechanical regulation, the goal of this work …

Corridors For Coexistence: Evaluating Spatiotemporal Impacts Of Livestock On Wildlife Community Dynamics, Edward Trout

Boise State University Theses and Dissertations

Livestock depredation by carnivores is a globally pervasive and detrimental interaction that leads to economic loss and retaliatory killings. Livestock trailed annually on US Rangelands impact wildlife communities- competing with ungulate herbivores for forage, disrupting predator-prey dynamics, and shifting community structures. In order to promote coexistence in these human-wildlife systems, a better understanding of how these processes interact is needed. However, studies on the topic fail to fully capture both the spatial and temporal signals of moving livestock herds.

In this study I investigated the effects of sheep grazing on a wildlife community in the Big Wood River Valley, Idaho. …

Understanding The Barriers And Facilitators Of Lidar Adoption For Flood Risk Management In The Pacific Northwest, U.S., Tara Pozzi

Boise State University Theses and Dissertations

The understanding of factors that influence technology adoption in emergency planners is foundational for ensuring resilient communities to hazards in the future. We explore these factors through an interdisciplinary, social-ecological science lens. In this thesis, we use cultural evolutionary theory to understand the facilitators and barriers of Light Detection and Ranging (lidar) adoption in flood risk management, as a case study of technology adoption for long-term risk mitigation. We then disseminate our findings through three educational outlets: a webinar, a white paper (Appendix A), and a Story Map. This thesis contributes to our intellectual understanding of technology adoption, as …

Engineering Of Recombinant Fortilin For Structure Activity Studies, Maranda S. Cantrell

Boise State University Theses and Dissertations

Cardiovascular disease (CVD) is the leading cause of death worldwide affecting approximately 40% of all adults over the age of 20 and is responsible for an economic burden upwards of $3 billion annually. Treatments for CVD are limited to either hypertension medication to treat symptoms, and/or statin-based drugs to reduce low-density lipoprotein (LDL) cholesterol formation. However, recent studies suggest that approximately 50% of patients diagnosed with CVD have normal to low LDL cholesterol levels. Therefore, a critical need exists to develop new treatments for CVD that are independent of cholesterol lowering statins. Fortilin, also known as translationally controlled tumor protein …

The 4d Living Genome, Julianna Arlene Sherman Goelzer

Boise State University Theses and Dissertations

Over the last few decades great advances have been made in our understanding of gene expression and the human genome. In 2003 the human genome was sequenced for the first time, allowing us to discover its true importance in human health. While sequencing the human genome was a great advance, it ultimately created more questions than it answered. It is known that the genomic sequence is extremely important in genome regulation, however recent studies have shown that the 4D (spatiotemporal) organization and dynamics of the living genome plays an equally critical role in regulation of gene expression. A key factor …

Collagen Xi Impact On Structure And Function Of The Vertebrate Inner Ear In A Zebrafish Model, Makenna Hardy

Boise State University Theses and Dissertations

The ear is essential to maintaining balance and hearing; both of which can be linked to one another and significantly impact a person’s quality of life. Although aging and damage are more common reasons for hearing loss, congenital ear defects still have a considerable impact on our population. The function of the ear can be affected by structural deformities to the ear and its components which results in hearing loss. Mutations and single nucleotide polymorphisms in the gene encoding Collagen XI alpha one chain (COL11A1) protein can play a role in hearing and balance dysfunction in humans as seen in …

Upscaling Tree Demography To Heterogenous Landscapes Using Models And Remote Sensing, Cristina Barber

Boise State University Theses and Dissertations

Tree demography is foundational to ecology and conservation, from mass tree die-offs to forest recovery. Plot-level studies of tree demography, including field measurements of tagged individuals, have been fundamental for developing ecological theory and forest management strategies. However, the limited spatial extent of field plots impedes generalizing plot-level models for spatial predictions across heterogeneous landscapes. Novel high-spatial resolution remote sensing imagery has opened the possibility for measuring tree demographic rates with continuous spatial coverage at landscape to regional extents. Remote sensing derived measurements could address pressing research questions, including disentangling causes of high variation in natural regeneration across secondary forest …

Prolonged Load Carriage Impacts Magnitude And Velocity Of Knee Adduction Biomechanics, Gaervyn John Salverda

Boise State University Theses and Dissertations

Introduction: Adopting knee adduction biomechanics during prolonged load carriage, a common military occupational activity, may increase service members knee osteoarthritis (OA) risk. Although service members reportedly increase knee adduction motions and moments during prolonged load carriage, it is unknown if either body borne load or walk duration increases velocity of knee adduction biomechanics, and subsequent knee OA risk. Varus thrust and alignment are also related to greater knee OA risk, yet it is unknown whether varus thrust and/or alignment are related to magnitude and velocity of knee adduction biomechanics during prolonged load carriage. Purpose: To determine whether body borne load …

How Wildlife Respond To Natural Noise, Dylan G. E. Gomes

Boise State University Theses and Dissertations

Animal sensory systems have evolved in a natural din of noise since the evolution of sensory organs. Anthropogenic noise is a recent addition to the environment, which has had demonstrable, largely negative, effects on wildlife. Yet, we know relatively little about how animals respond to natural sources of noise, which can differ substantially in acoustic characteristics from human-caused noise. Here we review the noise literature and suggest an evolutionary approach for framing the study of novel, anthropogenic sources of noise. We also push for a more quantitative approach to acoustic ecology research.

To build a better foundation around the effects …

New Applications For The Kinetic Exclusion Assay (Kinexa), Mark Harrison Smith

Boise State University Theses and Dissertations

This dissertation examines the fundamental principles and applicability of the kinetic exclusion assay (KinExA), developed and marketed by Sapidyne Instruments of Boise, Idaho, since 1995. Chapter One reviews and consolidates the manufacturer’s guidance and many early papers that delineate the practical and theoretical aspects of the technology. In brief, KinExA is a two stage analytical system. In stage one, a number of solutions are prepared, whereby one of the partners is kept constant (the constant binding partner, or CBP), while the other (the titrant) is varied, usually in serial dilution. As the titrant is increased, the free CBP decreases, and …

Natural Noise Can Influence Bird Foraging And Vigilance Behavior, Kate Antonia Sweet

Boise State University Theses and Dissertations

Natural sounds are an often overlooked, yet important component of an animal’s habitat. The acoustic environment may be especially significant during foraging, because a noisy world can limit auditory surveillance. Here, we investigated how natural noise structures the foraging vigilance trade-off to understand how intense acoustic environments may have shaped antipredator behavior across the evolutionary past, and better inform conservation efforts in the present.

First, in Chapter 1, I directly compared the foraging and vigilance behaviors of captive song sparrows (Melospiza melodia) in anthropogenic and natural noise. We recorded foraging trials in 4 playback conditions (roadway traffic, whitewater …

Movement Integration In Classrooms: Factors Associated With The Adoption And Implementation Of Physical Activity In Us Elementary Schools, R. Blake Densley

Boise State University Theses and Dissertations

The intentional integration of movement in elementary school classrooms—including both brief instructional breaks for physical activity, or the integration of physical activity with lessons—can benefit children’s physical health and education outcomes. Teachers are key implementation agents, but despite movement integration (MI) being considered an educational best practice, many classroom teachers do not regularly use it. The aim of this study was to obtain updated nationally-representative prevalence estimates in US public elementary schools, regarding four key outcomes pertaining to teachers’ implementation of physical activity: 1) school adoption of physically active lessons (PALs); 2) school adoption of physical activity breaks (PABs); 3) …

Effects Of A Mental Skills Training Program On Performance In Distance Runners, Neil Patrick Bangs

Boise State University Theses and Dissertations

Introduction: Anxiety affects nearly 20% of the United States population and can have a significant impact on athletic performance. However, athletes can mitigate the effects of performance anxiety through the use of mental skills and mindfulness training. Purpose: The aim of this study was to determine if a mental training and mindfulness intervention could reduce anxiety and enhance performance in distance runners. Hypothesis: The hypothesis of the study was that athletes would experience less anxiety, increased levels of mindfulness, and improved performance following a four-workshop intervention. Methods: Distance runners engaged in a mental skills and mindfulness training program where we …