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Articles 1 - 8 of 8
Full-Text Articles in Life Sciences
Complete Genome Sequences Of Cluster A Mycobacteriophages Bobswaget, Fred313, Kady, Lokk, Myradee, Stagni, And Stepmih, Kristen A. Butela, Susan M. R. Gurney, Heather L. Hendrickson, Janine M. Leblanc-Straceski, Anastasia M. Zimmerman, Stephanie B. Conant, Nikki E. Freed, Olin K. Silander, Joshua J. Thomson, Charlotte A. Berkes, Cristina Bertolez, Courtney G. Davies, Amber Elinsky, Alison J. Hanlon, Juliette Nersesyan, Payal Patel, John Sherwood, Tiffany Tieu Ngo, Kathryn A. Wisniewski, Kathrine Yacoo, Paul M. Arendse, Nathan W. Bowlen, Jasmina Cunmulaj, Julie L. Downs, Charlee A. Ferrenberg, Alexandra E. Gassman, Cody E. R. Gilligan, Emily Gorkiewicz, Christopher Harness, Anthony Huffman, Christina Jones, Anna Julien, Alexis E. Kupic, Sayonara F. Latu, Thomas J. Manning, Danielle Maxwell, Merrimack College Sea-Phages Annotators 2016, Catherine E. Meyer, Madeleine Reardon, Matthew Slaughter, Royce Swasey, Rebecca I. Tennent, Victoria Torres, Tamia Waller, Rachel M. Worcester, Brooke L. Yost, Steven G. Cresawn, Rebecca A. Garlena, Deborah Jacobs-Sera, Welkin H. Pope, Daniel A. Russell, Graham F. Hatfull, Jacob D. Kagey
Complete Genome Sequences Of Cluster A Mycobacteriophages Bobswaget, Fred313, Kady, Lokk, Myradee, Stagni, And Stepmih, Kristen A. Butela, Susan M. R. Gurney, Heather L. Hendrickson, Janine M. Leblanc-Straceski, Anastasia M. Zimmerman, Stephanie B. Conant, Nikki E. Freed, Olin K. Silander, Joshua J. Thomson, Charlotte A. Berkes, Cristina Bertolez, Courtney G. Davies, Amber Elinsky, Alison J. Hanlon, Juliette Nersesyan, Payal Patel, John Sherwood, Tiffany Tieu Ngo, Kathryn A. Wisniewski, Kathrine Yacoo, Paul M. Arendse, Nathan W. Bowlen, Jasmina Cunmulaj, Julie L. Downs, Charlee A. Ferrenberg, Alexandra E. Gassman, Cody E. R. Gilligan, Emily Gorkiewicz, Christopher Harness, Anthony Huffman, Christina Jones, Anna Julien, Alexis E. Kupic, Sayonara F. Latu, Thomas J. Manning, Danielle Maxwell, Merrimack College Sea-Phages Annotators 2016, Catherine E. Meyer, Madeleine Reardon, Matthew Slaughter, Royce Swasey, Rebecca I. Tennent, Victoria Torres, Tamia Waller, Rachel M. Worcester, Brooke L. Yost, Steven G. Cresawn, Rebecca A. Garlena, Deborah Jacobs-Sera, Welkin H. Pope, Daniel A. Russell, Graham F. Hatfull, Jacob D. Kagey
Biology Faculty Publications
Seven mycobacteriophages from distinct geographical locations were isolated, using Mycobacterium smegmatis mc2155 as the host, and then purified and sequenced. All of the genomes are related to cluster A mycobacteriophages, BobSwaget and Lokk in subcluster A2; Fred313, KADY, Stagni, and StepMih in subcluster A3; and MyraDee in subcluster A18, the first phage to be assigned to that subcluster.
Use Of Image Cytometry For Quantification Of Pathogenic Fungi In Association With Host Cells, Charlotte A. Berkes, Leo Li-Ying Chan, Alisha Wilkinson, Benjamin Paradis
Use Of Image Cytometry For Quantification Of Pathogenic Fungi In Association With Host Cells, Charlotte A. Berkes, Leo Li-Ying Chan, Alisha Wilkinson, Benjamin Paradis
Biology Faculty Publications
Studies of the cellular pathogenesis mechanisms of pathogenic yeasts such as Candida albicans, Histoplasma capsulatum, and Cryptococcus neoformans commonly employ infection of mammalian hosts or host cells (i.e. macrophages) followed by yeast quantification using colony forming unit analysis or flow cytometry. While colony forming unit enumeration has been the most commonly used method in the field, this technique has disadvantages and limitations, including slow growth of some fungal species on solid media and low and/or variable plating efficiencies, which is of particular concern when comparing growth of wild-type and mutant strains. Flow cytometry can provide rapid quantitative information regarding yeast …
Conidia But Not Yeast Cells Of The Fungal Pathogen Histoplasma Capsulatum Trigger A Type I Interferon Innate Immune Response In Murine Macrophages, Diane O. Inglis, Charlotte A. Berkes, Davina R. Hocking Murray, Anita Sil
Conidia But Not Yeast Cells Of The Fungal Pathogen Histoplasma Capsulatum Trigger A Type I Interferon Innate Immune Response In Murine Macrophages, Diane O. Inglis, Charlotte A. Berkes, Davina R. Hocking Murray, Anita Sil
Biology Faculty Publications
Histoplasma capsulatum is the most common cause of fungal respiratory infections and can lead to progressive disseminated infections, particularly in immunocompromised patients. Infection occurs upon inhalation of the aerosolized spores, known as conidia. Once inside the host, conidia are phagocytosed by alveolar macrophages. The conidia subsequently germinate and produce a budding yeast-like form that colonizes host macrophages and can disseminate throughout host organs and tissues. Even though conidia are the predominant infectious particle for H. capsulatum and are the first cell type encountered by the host during infection, very little is known at a molecular level about conidia or about …
Myod Synergizes With The E-Protein Heb Beta To Induce Myogenic Differentiation, Maura H. Parker, Robert L.S. Perry, Melanie C. Fauteux, Charlotte A. Berkes, Michael A. Rudnicki
Myod Synergizes With The E-Protein Heb Beta To Induce Myogenic Differentiation, Maura H. Parker, Robert L.S. Perry, Melanie C. Fauteux, Charlotte A. Berkes, Michael A. Rudnicki
Biology Faculty Publications
The MyoD family of basic helix-loop-helix transcription factors function as heterodimers with members of the E-protein family to induce myogenic gene activation. The E-protein HEB is alternatively spliced to generate alpha and beta isoforms. While the function of these molecules has been studied in other cell types, questions persist regarding the molecular functions of HEB proteins in skeletal muscle. Our data demonstrate that HEB alpha expression remains unchanged in both myoblasts and myotubes, whereas HEB beta is upregulated during the early phases of terminal differentiation. Upon induction of differentiation, a MyoD-HEB beta complex bound the E1 E-box of the myogenin …
The Lift Pool Method For Isolation Of Cdna Clones From Lambda Phage Libraries, Janine M. Leblanc-Straceski, Pablo Sobrado, Sharon Betz, Julie Zerfas, Karen Morgan
The Lift Pool Method For Isolation Of Cdna Clones From Lambda Phage Libraries, Janine M. Leblanc-Straceski, Pablo Sobrado, Sharon Betz, Julie Zerfas, Karen Morgan
Biology Faculty Publications
A PCR based strategy was developed to screen a Xenopus oocyte λgt10 cDNA library. The PCR-based lift pool (LP) method follows the same two tiered strategy as conventional screening of phage libraries by filter hybridization. Two rounds of plating, one at high density to detect the clone, and one at low density to purify the clone to homogeneity, are performed. In the first round, lysates from high density plates, termed plate pools (PP), serve as template for PCR. In the second round, phage particles adhering to plaque lifts of low density plates are washed off nitrocellulose filters to create LPs, …
Myod Targets Chromatin Remodeling Complexes To The Myogenin Locus Prior To Forming A Stable Dna-Bound Complex, Ivana L. De La Serna, Yasuyuki Ohkawa, Charlotte A. Berkes, Donald A. Bergstrom, Caroline S. Dacwag, Stephen J. Tapscott, Anthony N. Imbalzano
Myod Targets Chromatin Remodeling Complexes To The Myogenin Locus Prior To Forming A Stable Dna-Bound Complex, Ivana L. De La Serna, Yasuyuki Ohkawa, Charlotte A. Berkes, Donald A. Bergstrom, Caroline S. Dacwag, Stephen J. Tapscott, Anthony N. Imbalzano
Biology Faculty Publications
The activation of muscle-specific gene expression requires the coordinated action of muscle regulatory proteins and chromatin-remodeling enzymes. Microarray analysis performed in the presence or absence of a dominant-negative BRG1 ATPase demonstrated that approximately one-third of MyoD-induced genes were highly dependent on SWI/SNF enzymes. To understand the mechanism of activation, we performed chromatin immunoprecipitations analyzing the myogenin promoter. We found that H4 hyperacetylation preceded Brg1 binding in a MyoD-dependent manner but that MyoD binding occurred subsequent to H4 modification and Brg1 interaction. In the absence of functional SWI/SNF enzymes, muscle regulatory proteins did not bind to the myogenin promoter, thereby providing …
Multiple Members Of A Third Subfamily Of P-Type Atpases Identified By Genomic Sequences And Ests, Margaret S. Halleck, Deepti Pradhan, Christie Blackman, Charlotte A. Berkes, Partrick Williamson, Robert A. Schlegel
Multiple Members Of A Third Subfamily Of P-Type Atpases Identified By Genomic Sequences And Ests, Margaret S. Halleck, Deepti Pradhan, Christie Blackman, Charlotte A. Berkes, Partrick Williamson, Robert A. Schlegel
Biology Faculty Publications
The Saccharomyces cerevisiae genome contains five P-type ATPases divergent from both of the well-known subfamilies of these membrane ion transporters. This newly recognized third subfamily can be further divided into four classes of genes with nearly equal relatedness to each other. Genes of this new subfamily are also present and expressed in multicellular organisms such as Caenorhabditis elegans and mammals; some, but not all, can be assigned to the classes identified in yeast. Different classes of genes and different genes within a class are expressed differentially in tissues of the mouse. The recently cloned gene for the mammalian aminophospholipid translocase …
Mutations In The Non-Helical Linker Segment L1-2 Of Keratin 5 In Patients With Weber-Cockayne Epidermolysis Bullosa Simplex, Yiu-Mo Chan, Qian-Chun Yu, Janine M. Leblanc-Straceski, Angela Christiano, Lena Pulkkinen, Raju S. Kucherlapati, Jouni Uitto, Elaine Fuchs
Mutations In The Non-Helical Linker Segment L1-2 Of Keratin 5 In Patients With Weber-Cockayne Epidermolysis Bullosa Simplex, Yiu-Mo Chan, Qian-Chun Yu, Janine M. Leblanc-Straceski, Angela Christiano, Lena Pulkkinen, Raju S. Kucherlapati, Jouni Uitto, Elaine Fuchs
Biology Faculty Publications
Keratins are the major structural proteins of the epidermis. Analyzing keratin gene sequences, appreciating the switch in keratin gene expression that takes place as epidermal cells commit to terminally differentiate, and elucidating how keratins assemble into 10 nm filaments, have provided the foundation that has led to the discoveries of the genetic bases of two major classes of human skin diseases, epidermolysis bullosa simplex (EBS) and epidermolytic hyperkeratosis (EH). These diseases involve point mutations in either the basal epidermal keratin pair, K5 and K14 (EBS), or the suprabasal pair, K1 and K10 (EH). In severe cases of EBS and EH, …