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Full-Text Articles in Life Sciences
Cold Rearing Improves Cold-Flight Performance In Drosophila Via Changes In Wing Morphology, Melanie R. Frazier, Jon F. Harrison, Scott D. Kirkton, Stephen P. Roberts
Cold Rearing Improves Cold-Flight Performance In Drosophila Via Changes In Wing Morphology, Melanie R. Frazier, Jon F. Harrison, Scott D. Kirkton, Stephen P. Roberts
Biological Sciences Faculty Research & Creative Works
We Use a Factorial Experimental Design to Test Whether Rearing at Colder Temperatures Shifts the Lower Thermal Envelope for Flight of Drosophila Melanogaster Meigen to Colder Temperatures. D. Melanogaster that Developed in Colder Temperatures (15°C) Had a Significant Flight Advantage in Cold Air Compared to Flies that Developed in Warmer Temperatures (28°C). at 14°C, Cold-Reared Flies Failed to Perform a Take-Off Flight ∼47% of the Time Whereas Warm-Reared Flies Failed ∼94% of the Time. at 18°C, Cold- and Warm-Reared Flies Performed Equally Well. We Also Compared Several Traits in Cold- and Warm-Developing Flies to Determine If Cold-Developing Flies Had Better …
Revisiting A Model Of Ontogenetic Growth: Estimating Model Parameters From Theory And Data, Melanie E. Moses, Chen Hou, William H. Woodruff, Geoffrey B. West, Jeffery C. Nekola, Wenyun Zuo, James H. Brown
Revisiting A Model Of Ontogenetic Growth: Estimating Model Parameters From Theory And Data, Melanie E. Moses, Chen Hou, William H. Woodruff, Geoffrey B. West, Jeffery C. Nekola, Wenyun Zuo, James H. Brown
Biological Sciences Faculty Research & Creative Works
The ontogenetic growth model (OGM) of West et al. provides a general description of how metabolic energy is allocated between production of new biomass and maintenance of existing biomass during ontogeny. Here, we reexamine the OGM, make some minor modifications and corrections, and further evaluate its ability to account for empirical variation on rates of metabolism and biomass in vertebrates both during ontogeny and across species of varying adult body size. We show that the updated version of the model is internally consistent and is consistent with other predictions of metabolic scaling theory and empirical data. The OGM predicts not …
Nuclear Polyglutamine-Containing Protein Aggregates As Active Proteolytic Centers, Min Chen, Lena Singer, Andrea Scharf, Anna Von Mikecz
Nuclear Polyglutamine-Containing Protein Aggregates As Active Proteolytic Centers, Min Chen, Lena Singer, Andrea Scharf, Anna Von Mikecz
Biological Sciences Faculty Research & Creative Works
Protein aggregates and nuclear inclusions (NIs) containing components of the ubiquitin-proteasome system (UPS), expanded polyglutamine (polyQ) proteins, and transcriptional coactivators characterize cellular responses to stress and are hallmarks of neurodegenerative diseases. The biological function of polyQ-containing aggregates is unknown. To analyze proteasomal activity within such aggregates, we present a nanoparticle (NP)-based method that enables controlled induction of sodium dodecyl sulfate-resistant inclusions of endogenous nuclear proteins while normal regulatory mechanisms remain in place. Consistent with the idea that the UPS maintains quality control, inhibition of proteasomal proteolysis promotes extra large protein aggregates (1.4-2 μm), whereas formation of NP-induced NIs is found …
Validation Of An Nsp-Based (Negative Selection Pattern) Gene Family Identification Strategy, Ronald L. Frank, Cyriac Kandoth, Fikret Erçal
Validation Of An Nsp-Based (Negative Selection Pattern) Gene Family Identification Strategy, Ronald L. Frank, Cyriac Kandoth, Fikret Erçal
Biological Sciences Faculty Research & Creative Works
Background: Gene family identification from ESTs can be a valuable resource for analysis of genome evolution but presents unique challenges in organisms for which the entire genome is not yet sequenced. We have developed a novel gene family identification method based on negative selection patterns (NSP) between family members to screen EST-generated contigs. This strategy was tested on five known gene families in Arabidopsis to see if individual paralogs could be identified with accuracy from EST data alone when compared to the actual gene sequences in this fully sequenced genome. Results: The NSP method uniquely identified family members in all …