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Investigating The Roles Of Rna Modification And Associated Enzymes On Stress Response And Connections To Human Diseases, Carrson Widen

Biological Sciences

Transfer RNAs decode messenger RNA using anticodon – codon interactions to deliver specific amino acids to a growing peptide chain. Enzyme-catalyzed modifications on specific nucleotides on tRNAs increase their stability and help mediate the speed and fidelity of translation, giving these modifications regulatory potential. RNA modifications are catalyzed by writer enzymes, and I predicted that writers are important in the cellular responses to stress. I have evaluated the following E. coli modification enzyme mutants: miaA, mnmE, dtd, tmcA, lysU, yeaK, and iscS, from the corresponding Keio E. coli gene deletion library. Writer mutants were exposed to hydrogen peroxide to induce …