Life Sciences Commons^™

Role Of Iron In The Mechanism Of Asbestos-Induced Apoptosis In Human Lung And Pleural Target Cells, Aleksander Baldys

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Occupational exposure to asbestos has been associated with increased incidence of pulmonary interstitial fibrosis, mesothelioma of the pleura, and bronchogenic carcinoma. Although the mechanism by which asbestos causes cancer remains unknown, iron associated with asbestos is thought to play a role in the pathogenic effects of fibers.

The aim of this research was to examine and compare the asbestos-induced signaling phenomena in relevant human lung and pleural target cells, and to determine the role of iron from asbestos fibers in these events. Exposure of human airway epithelial (A549) cells, human pleural mesothelial (MET5A) cells, and normal human small airway epithelial …

Relative Absorption Of Iron And Magnesium From Sulfate Salts, Amino Acid Chelates Complexed And/Or Mixed With Vegetables, And Taste-Free Supplements, Jennifer A. Bowden

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Common methods for determining mineral absorption by the body are invasive and frequently utilize radioisotopes. In experiment one. rats were given a dose of radiolabeled ferrous sulfate. Relative absorption was estimated by changes in serum iron and by appearance of the radiotracer in the serum. There were no differences in relative absorption determined by the methods, although the low overall absorptions by the iron-replete rats may have decreased the sensitivity.

In experiments two and three. iron and magnesium supplements were given orally to 12 women age 19-25. Each subject received iron and magnesium supplements once each week for 5 weeks. …

Fats And Iron In The Rat Colon: Effects On Lipid Peroxidation, Ji Jenny Chin

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Preliminary studies were undertaken to investigate whether or not added iron (0, 35, 880 ppm of iron as ferrous fumarate) and fat type (corn oil, beef tallow, or menhaden oil) influenced the oxidation of the rat diet during storage. Iron level affected thiobarbituric acid (TBA) values only in the menhaden oil diets. Storage for 4 d did not affect TBA values of the diets. Neither food intake nor body weight of the rats was affected by the different diets, suggesting all diets were equally acceptable to rats.

The effects of iron supplementation and fat type on in vivo lipid peroxidation …

Mobilization Of Iron Enhances The Iron-Dependent Biochemical Reactivity Of Asbestos In Vitro And Contributes To The Cytotoxicity Of Asbestos In Cultured Cells, Loren Glen Lund

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Asbestos related research began approximately 60 years ago, yet, the mechanism(s) by which asbestos exerts its biological effects is not well understood. The hypothesis upon which this dissertation is based is that mobilization of iron from asbestos enhances the iron-dependent biochemical reactivity of asbestos in vitro and contributes to asbestos-dependent cytotoxicity. The specific aims for this hypothesis were, 1) to determine whether iron was responsible for the biochemical reactivity of asbestos in vitro and asbestos-induced cytotoxicity in cultured cells, and 2) to determine whether mobilization of iron from asbestos enhanced the reactions catalyzed by asbestos in vitro and contributes to …

Selenium And Iron In The Rat Intestine: Effects On Lipid Peroxidation, Sileny Vega

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Effects of Fe and Se status on GSHPx activity and lipid peroxidation in liver and intestinal mucosa were studied. Rats were Se and Fe supplemented (+Se+Fe), Se-deficient and Fe-supplemented (-Se+Fe), Se supplemented and Fe overloaded (+Se++Fe) by intramuscular injection, or Se deficient and Fe overloaded (-Se++Fe) for 20d. Fe overloaded tissues had more Fe, but hemoglobin was unaffected. Liver and mucosal GSHPx activity was low in Se-deficient rats. Thiobarbituric acid reactive substances (TBARS) were higher in Fe overloaded and -Se++Fe vs +Se++Fe tissues. Mucosal TBARS was higher in -Se++Fe rats gavaged with CBrCl₃. In experiment 2, Fe overload …

Change In Serum Iron As A Measure Of Bioavailability Of Dietary Iron, Mary Beth Wright

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Methods currently used to determine iron bioavailability have disadvantages for both the subjects and researchers involved. One safe and simple method that needs further evaluation, the serum iron absorption method, involves an initial blood drawing, dosages of iron, and blood drawings taken at intervals thereafter. Generally, the rise in serum iron or area under the curve is used to determine iron uptake. Two experiments were conducted using the serum iron absorption method in an effort to improve the utility of this method for the measurement of iron bioavailability. With this effort in mind, an equation adjusting for dose of iron …

A Longitudinal Study Of Dietary Intake And Influence Of Iron Status On Infants' And Toddlers' Mental And Motor Development, Steven Matley Wood

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Thirty-two non-anemic children were assessed clinically, biochemically and anthropometrically at six months, one year and two years of age to investigate the relationship of iron status to mental and motor development. Using dietary history and dietary record instruments, dietary iron was estimated and compared to the National Food Consumption Survey (1985) and the Recommended Dietary Allowances (RDA) for corresponding age groups. The Bayley Scales of Infant Development (which assess mental and motor development) and the Caldwell Home Inventory (which evaluates the environmental stimuli) were performed and correlated with blood iron parameters, anthropometric measurements and dietary nutrient intakes.

Nutrient intakes of …

Maternal Iron Parameters And Diet: Influence On Infant Iron Parameters And Development, Janet Bradshaw Anderson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The purpose of this study was to determine and evaluate the dietary intake of pregnant women living in this geographical area, evaluate how their diets related to their iron status, how their nutrient intakes compared with other studies, and how their iron status and diet affected infant iron status and development, as assessed by the Bayley Scale of Infant Development.

The study involved 32 pregnant women who were between 8 to 14 weeks gestation and 20 to 35 years of age. They were followed through pregnancy and their infants were followed until three months of age.

Three-day dietary records, a …

The Bioavailability Of Iron From Meat, Spinach, Soy Protein Isolate, Meat:Spinach And Meat:Spi Mixtures Fed To Anemic And Healthy Rats, Dejia Zhang

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Two Experiments were conducted to investigate the effect of iron status and iron source on iron bioavailability using the rat model. Beef was proportionally mixed with spinach (Experiment I) or soy protein isolate (Experiment II) to test if meat enhances the absorption of iron from spinach or SPI. Five diets with the iron from meat:spinach (or SPI) ratios of 100:0, 75:25, 50:50, 25:75 and 0:100 were prepared and fed to rats. FeS04 was used as a reference. Two trials of rats were used in Experiment I. In trial I, half of the rats were made severely iron deficient (Hb 6.3 …

Iron Bioavailability Of Meat:Bread Mixtures And Meat Loaves Fed To Anemic And Healthy Rats, Abdullah M. Thannoun

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

To study the effect of meat (beef) on dietary iron bioavailability from enriched white bread (EWB) or whole wheat bread (WWB), diets were prepared in which the ratios of beef iron to bread iron were 100:0, 75:25, 50:50, 25:75 or 0:100. Hemoglobin regeneration efficiency (HRE), apparent iron absorption, and dry matter absorption were determined using weanling anemic and healthy male rats.

Meat iron was more available than EWB or WWB. Fortification iron in EWB was less available than the iron naturally present in WWB. At the iron dose given, HRE was similar for both anemic and healthy rats. Although healthy …

Effect Of Iron On Residual Nitrite Level In Ground Pork And Model Systems, Changmin Kim

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The effects of iron, temperature, and presence of botulinal spores or sodium ascorbate on depletion of nitrite level were determined in meat and model systems. Higher temperature and presence of botulinal spores definitely increased nitrite depletion in a meat system. Added hemoglobin also significantly increased nitrite depletion, while ferrous iron and ferric iron did not significantly decrease nitrite level in a meat system. High temperature and presence of sodium ascorbate increased nitrite depletion in a model system. Only ferrous iron significantly decreased nitrite level in the absence of ascorbate, while ferric iron, heme iron, and ferritin iron did not decrease …

The Isolation And Characterization Of A Novel Iron Protein From Desulfovibrio Gigas, Alan Kay Smith

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The isolation, purification, and partial characterization of a novel iron-containing protein from the sulfate-reducing anaerobic bacterium, Desulfovibrio gigas, is described. The highly insoluble protein was isolated from the cell debris following osmotic shock of the bacteria. The insoluble fraction consistently contained about 90% of the cell-associated iron. This protein was treated with acid, chelating agents, detergents and proteases in order to study their effect on the iron solubility. The results of elemental analysis of a crude protein preparation were 5.3% iron, 2.9% sulfur and 11 .9% nitrogen. An independent colorimetric iron analysis showed 6.4% iron. The iron could be …

The Transport And Intracellular Distribution Of Iron In The Basidiomycete, Ustilago Sphaerogena, Charles W. Passavant

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The transport and intracellular distribution of iron in Ustilago sphaerogena via the native siderochrome ferrichrome A has been examined. Evidence is presented which suggests the involvement of deferriferrichrome in iron transport when the metal is supplied to the cell as ferrichrome A. This process requires both oxygen and ATP synthesis and may require a transmembrane electrochemical gradient. Once inside the cell, iron derived from ferrichrome A is rapidly distributed, with a major fraction found in the mitochondria, presumably as heme.

The kinetics of ferrichrome and ferrichrome A uptake have also been investigated. The Km of transport of ferrichrome was …

Iron And Zinc Deficiencies In Selected Calcareous Soils Of Southern Utah, E. Frank Schnitzer

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The response of field corn to iron and zinc fertilization was studied using a split plot experimental design in Millard County, Utah, in cooperation with the Utah State University Extension Agent and a local farmer. mainplot treatment applications consisted, on an acre basis, of (1) 5 tons of sulfuric acid, (2) 1 ton sulfuric acid, (3) 1.8 tons gypsum, (4) check plot. Subplot treatments were (1) Fe at 5 lbs/Ac, (2) Zn at 10 lbs/Ac, (3) Fe and Zn at 5 and 10 lb / Ac, respectively, (4) check plot. The iron and zinc applications were essentially rendered unavailable by …

The Iron Bioavailability Of Mechanically-Deboned Meats, Bonnie Rae Anderson Farmer

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Two separate experiments were used to investigate iron bioavailability of mechanically-deboned meats using hemoglobin regeneration to measure iron utilization. In both studies, male weanling rats were made anemic by bleeding and being fed a low-iron diet. Experimental diets were control fed to the animals and final hemoglobin concentrations were recorded. Animals were sacrificed and liver iron concentrations determined. The first experiment measured the iron bioavailability of mechanically-deboned and hand-deboned beef plate was better utilized by rats than the iron from mechanically- deboned shank and mechanically-deboned plate. The effect of lipid source and level on iron bioavailability of mechanically-deboned turkey meat …

The Relationship Of Molybdenum To Iron Status In Pregnancy And Anemia In Rats And Humans, Jo Ann Mortensen

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Weanling male rats were made anemic and fed diets supplemented with 20 ppm iron and/or 2 ppm molybdenum. A decrease in serum iron was observed in the rats supplemented only with iron and a significant decrease in hemoglobin was observed in rats given no supplementation.

In a second experiment, pregnant female rats were also fed diets supplemented with 20 ppm iron and/or 2 ppm molybdenum. An inverse relationship was apparent between iron and copper in both the serum and the liver of the female rats. The livers of their pups displayed an inverse relationship between molybdenum and copper. Hemoglobin in …

Some Effects Of Mechanical Deboning On The Composition And The Bioavailability Of Protein And Iron In Turkey Frame Meat, Lowell C. Allred

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Meat was obtained from a single daily lot of turkey frames by mechanically deboning with a Beehive AU 4171 deboner and by hand deboning. The meat was analyzed for protein, fat, moisture, ash, iron, calcium, and 19 amino acids. Mechanically deboned turkey had 68 percent more ash, 74 percent more iron, and nine times more calcium than the hand deboned turkey meat. Other constituents were about equal in the two products. Protein bioavailability tests, including protein efficiency ratio, biological value, net protein utilization, and nitrogen efficiency for growth, did not reveal significant differences between mechanically deboned and hand deboned turkey. …

Effects Of X-Ray Induced Achlorhdria On The Utilization Of Dietary Calcium And Iron From Different Salts By Rats, Reid Scott Holbrook

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Dietary calcium has been classified as one of the minerals frequently limiting in the American diet (FAO, 1962) . Bone demineralization has been observed as a result of calcium deficient diets (Salomon et al., 1972), partial gastrectomies (Eddy, 1971) and inadequate hormonal balance (Albright et al., 1948). Although bone demineralization, or osteoporosis, has been attributed to many factors, the interrelationship of gastric acidity and the utilization of dietary calcium may be a key to the etiology of osteoporosis.

Eighty weanling male albino rats were divided into eight groups. All animals had their stomachs exposed through a mid-line incision. Control rats …

Effects Of Zinc Deficiency On Hemoglobin, Red Cell Counts, Red Cell Fragility And Circulating And Storage Levels Of Zinc, Iron And Copper In The Rat, Rekha R. Dalvi

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Three experiments were conducted to study the effect of zinc deficiency on hemoglobin, red cell counts, red cell fragility, storage of erythropoietic minerals in livers and circulating levels of zinc, iron and copper in the rat.

In the experiment 1, twenty weanling rats were divided into four groups. Two groups were fed zinc-deficient diet ( <0.5 ppm); and two groups, zinc-supplemented diet (50 ppm). The rats in the first and third groups were bled three times a week (bled), however the rats in remaining groups were bled once a week (non-bled). All rats received ad libitum food.

Mean food intake per day was considerably less for zinc-deficient rats than that for zinc-supplemented rats. Approximately 2 to 3 times as much food was required for each gram of body weight gained by the zinc-deficient group as by the controls.

In the experiment 2, …

Methods Of Correcting Iron And Zinc Deficiencies In Peach Trees, Earl Seeley

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Studies were conducted to determine the effects of iron and zinc chelates on these deficiencies. Soil treatments, and foliar treatments with and without urea were used. It was found that in soil treatments at least one pound chelate per tree must be used to significantly increase the iron content of the leaves. The soil treatments did not result in as high a concentration in the leaves early in the season, but they had a residual effect that lasted throughout the entire growing season. Foliar treatments with iron did not effect the foliage that developed after the final application was made. …

A Study Of The Off-Farm Agriculture Occupations Of Millard, Beaver, Iron, And Washington Counties In Utah, To Determine Educational Needs, Thales C. Brown

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The objectives of this study were to gather information concerning the off-farm agricultural occupations of Millard, Beaver, Iron and Washington Counties.

A list was made of present and anticipated number of employees in these occupations with the numbers involved in full-time, part-time, and female work.

Occupations with similar backgrounds were clustered into four main areas. These were further classified into individual jobs.

Comments were made concerning the competencies needed for entry and satisfactory performance in these occupations.

Some of the characteristics of these occupations as to salary, required experience, labor laws and education requirements were reported.

An attempt was made …

The Effect Of Various Levels Of Bicarbonate, Phosphorus, And Ph On The Translocation Of Iron In Plants, Ronald Conrad Doney

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Line-induced chlorosis is a nutritional disease affecting many fruit trees, berries, ornamental shrubs, and flowers in Utah and the western United States. In fact, this disease is of wide distribution on calcareous soils throughout the world. Lime-induced chlorosis has contributed to untold economic loss and has challenged the technical ingenuity of plant and soil scientists throughout the world. As yet the exact cause of chlorosis has not been determined; consequently, no permanent cures have been found.

The first symptoms of this disease are characterized by an interveinal yellowing of younger leaves, with the veins remaining green. In more severe cases …