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All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

1997

Bifidobacterium bifidum

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Isolation And Biochemical Characterization Of Acid- And Bile- Tolerant Strains Of Lactobacillus Acidophilus And Bifidobacterium Bifidum, Lan-Szu Chou May 1997

Isolation And Biochemical Characterization Of Acid- And Bile- Tolerant Strains Of Lactobacillus Acidophilus And Bifidobacterium Bifidum, Lan-Szu Chou

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Lactic acid bacteria have been reported to be used as a health adjunct in food for u many years. However, these health benefits have not been proven. and how these bacteria pass through the digestion process and remain viable in the human intestinal tract is still not clear. The aim of this work was to isolate mutants from Lactobacillus acidophilus or Bifidobacterium bifidum that could tolerate the conditions of the digestion process (low pH and bile conduction) and to characterize these isolated mutants.

Acid- and bile-tolerant mutants of L. acidophilus were isolated from parental strains successfully using natural selection techniques. …