Life Sciences Commons^™

Evaluation Of Allergic Hypersensitivity To 2,4-D, Malathion, And Captafol In Mice, Janette R. Cushman

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

2,4-D-specific IgE antibodies were detected in serum of BALB/c mice using the rat passive cutaneous anaphylaxis (PCA) test following the second intraperitoneal immunization with 1, 10, or 100 ug of 2,4-D0keyhole limpet hemocyanin (KLH) conjugate administered with aluminum hydroxide adjuvant. The groups that received 1 ug of 2,4-D conjugate had the highest antibody titers (geometric mean of 60). A paper radioallergosorbent test (PRAST) was developed for determination of 2,4-D-specific IgE. The PRAST was equally as sensitive as and showed a positive correlation with the PCA assay.

The anhydride of the diacid metabolite of malathion (MMA) coupled to KLH elicited MMA-specific …

Toxicologic Effects Of Extracts Of Cestrum Parqui L'Héritier In Mice, Tomás A. López

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Cestrum parqui L'Héritier is a Solanaceous weed of wide distribution throughout the world. It causes poisoning of animals, particularly cattle and sheep, and constitutes a serious problem for animal production in some countries.

The present study was undertaken to develop a biological test for the detection of toxicity of extracts obtained from the plant. This test will be utilized to assess the toxicity of extracts obtained from the plant in future studies.

The plant is known to induce poisoning in laboratory animals, but there are no precise references on doses to be utilized and of appropriate routes of dosing. Oral …