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Coral Larvae For Restoration And Research: A Large-Scale Method For Rearing Acropora Millepora Larvae, Inducing Settlement, And Establishing Symbiosis, F. Joseph Pollock, Sefano M. Katz, Jeroen A.J.M. Van De Water, Sarah W. Davies, Margaux Hein, Gergely Torda, Mikhail V. Matz, Victor H. Beltran, Patrick Buerger, Eneour Puill-Stephan, David Abrego, David G. Bourne, Bette L. Willis
Coral Larvae For Restoration And Research: A Large-Scale Method For Rearing Acropora Millepora Larvae, Inducing Settlement, And Establishing Symbiosis, F. Joseph Pollock, Sefano M. Katz, Jeroen A.J.M. Van De Water, Sarah W. Davies, Margaux Hein, Gergely Torda, Mikhail V. Matz, Victor H. Beltran, Patrick Buerger, Eneour Puill-Stephan, David Abrego, David G. Bourne, Bette L. Willis
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© 2017 Pollock et al. Here we describe an efficient and effective technique for rearing sexually-derived coral propagules from spawning through larval settlement and symbiont uptake with minimal impact on natural coral populations. We sought to maximize larval survival while minimizing expense and daily husbandry maintenance by experimentally determining optimized conditions and protocols for gamete fertilization, larval cultivation, induction of larval settlement by crustose coralline algae, and inoculation of newly settled juveniles with their dinoflagellate symbiont Symbiodinium. Larval rearing densities at or below 0.2 larvae mL-1 were found to maximize larval survival and settlement success in culture tanks while minimizing …