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Quantitative Analysis Of Glycinergic Neurons Including Ia Inhibitory Interneurons In The Ventral Spinal Cord Using A Bac-Glyt2-Egfp Transgenic Mouse Model, Palak Rajeshkumar Painter
Quantitative Analysis Of Glycinergic Neurons Including Ia Inhibitory Interneurons In The Ventral Spinal Cord Using A Bac-Glyt2-Egfp Transgenic Mouse Model, Palak Rajeshkumar Painter
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We are interested in understanding the development of reciprocal inhibition, an important sensory-motor circuit that prevents co-contraction of antagonist muscles during locomotion. This effect is mediated by a subset of glycinergic neurons termed Ia inhibitory interneurons (IaINs). Transgenic mouse models are useful in helping to identify subsets of neurons in developing animals. In this thesis we first wanted to test the BAC-GlyT2-eGFP mice model by measuring GFP expression overlap in neurons with glycine in late-stage embryos (E17.5) and early postnatal animals (P0/P1). Our results suggest more than 98% of glycinergic neurons at these stages also express GFP in GlyT2-EGFP transgenic …
Hcn1 Immunoreactivity Of Α-Motoneurons Following Peripheral Nerve Injury, Saif Ahmed
Hcn1 Immunoreactivity Of Α-Motoneurons Following Peripheral Nerve Injury, Saif Ahmed
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Peripheral axotomy causes significant alterations in intrinsic motoneuron activity and excitability. Despite successful reinnervation of peripheral targets after injury, the recovery of motor function is incomplete. Following axotomy in the adult cat, there is an increase in the afterhyperpolarization (AHP) of the action potential in specific motoneuron types. AHP duration can be inversely correlated with the amount of Ih or sag current and further shaped through SK (Small conductance calcium-activated potassium) channel currents (Gustafsson and Pinter, 1985; Kuno, Miyata, and Muñoz-Martinez, 1974a). Hyperpolarizing-cyclic nucleotide gated (HCN) channels underlie sag currents and are critical to neuronal function by their unique property …
Comparing Two Different Statins In A Delayed Pharmacological Treatment For Ischemic Stroke, Kailyn M. Hagerty
Comparing Two Different Statins In A Delayed Pharmacological Treatment For Ischemic Stroke, Kailyn M. Hagerty
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Stroke is one of the leading causes of death and the top cause of long term disability. Currently there exists no standardized treatment of care for ischemic stroke patients during the days following stroke other than giving aspirin. A drug combination containing fluoxetine, one of two statins, and ascorbic acid was used to try and improve motor function following strokes in rats. Endothelin induced stroke survival surgery was performed on the right cortex of Long Evans and Sprague Dawley rats. Functional tests were completed pre- and post-surgery and immunohistochemistry was carried out to analyze infarct volume, angiogenesis, and the glial …
The Use Of Forelimb Asymmetry Functional Tests To Determine Motor Recovery With Various Drug Treatments Following Endothelin-Induced Stroke, Kelly Rebecca Leach
The Use Of Forelimb Asymmetry Functional Tests To Determine Motor Recovery With Various Drug Treatments Following Endothelin-Induced Stroke, Kelly Rebecca Leach
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Our laboratory has found increased functional recovery post-stroke with Long Evans rats that received a combination of fluoxetine, simvastatin, and vitamin C. The objectives of this study were to test that treatment and other drug combinations on Sprague Dawley rats, to determine whether Forelimb Asymmetry is a reliable behavioral measure for stroke studies, and to determine the most optimal surgical procedure on Sprague Dawley rats. There were more significant increases in functional recovery observed on the Forelimb Asymmetry than on Montoya Staircase behavioral tests, but there were no significant differences between treatment groups and control. The largest number of rats …
Expressing Human Orai3 In Insect Cells For Pharmacological Studies, Orville R. Bennett
Expressing Human Orai3 In Insect Cells For Pharmacological Studies, Orville R. Bennett
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The Orai3 protein forms a Ca2+ channel with a pharmacological profile distinct from its close relative, the store-operated Ca2+ channel Orai1. Though closely related to Orai1, the function of Orai3 in humans is still unclear. This study attempts to contribute to the body of knowledge by undertaking a pharmacological analysis of Orai3 in insect cells. We describe here the creation of a vector capable of expressing the mammalian Orai3 gene on an insect background. We demonstrate the ability to induce gene expression of Orai3 in these insect cells, and then assess the effectiveness of this system by characterizing the pharmacological …