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Articles 1 - 30 of 44
Full-Text Articles in Life Sciences
Effect Of Oasis-Ultra Matrix On The Healing Rate Of Stage Iv Pressure Wounds, Abdelfatah Shaban Abou Issa
Effect Of Oasis-Ultra Matrix On The Healing Rate Of Stage Iv Pressure Wounds, Abdelfatah Shaban Abou Issa
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Introduction: (Oasis-ultra) is an extra cellular collagen rich matrix derived from porcine intestinal sub-mucosa. A prospective, multi-centered, randomized, single-blinded clinical trial was conducted to study the effects of Oasis-ultra combined with negative pressure wound therapy (NPWT) on the healing rate of stage IV pressure wounds versus NPWT alone. Materials and Methods: Twelve subjects were involved in the study: six patients in the study group and six in the control group. NPWT was changed twice a week for all subjects, and Oasis-ultra was applied weekly. The wounds were measured weekly, and the healing rate was calculated for each subject for 12 …
Examination Of A Post-Stroke Drug Treatment For Its Effect On Blood Brain Barrier Permeability, And Gene Expression Changes In The Peri-Infarct Region, Ankita Anil Patel
Examination Of A Post-Stroke Drug Treatment For Its Effect On Blood Brain Barrier Permeability, And Gene Expression Changes In The Peri-Infarct Region, Ankita Anil Patel
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In this current study, we have investigated this a combination of fluoxetine, simvastatin and ascorbic acid administered daily beginning at 20-26 hours after stroke induction. We hope to understand therapeutic abilities by studying its effectiveness on the blood brain barrier permeability and gene expression changes of the microglial subtypes involved in neuro-inflammation and neurogenesis factors in the peri-infarct region. Our results indicate that S-enantiomer of fluoxetine may be more beneficial compared to the R-enantiomer. The S-enantiomer was effective in tightening the blood brain barrier in contrast to the R-enantiomer, in which the latter showed a greater Evans Blue dye permeability …
Effects Of Synthetic Ligands On Heterodimer Pairs Regarding Full-Length Human Pparα, Rxrα And Lxrα, Emily Delman
Effects Of Synthetic Ligands On Heterodimer Pairs Regarding Full-Length Human Pparα, Rxrα And Lxrα, Emily Delman
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Nuclear receptor study is critically relevant in therapeutic medicine since the intricate details of disease states pertaining to atherosclerosis and diabetes are poorly understood. Three nuclear receptors of interest regulate target genes pertaining to cholesterol and fatty acid regulation, linking these receptors to therapeutic medicine. The first is the peroxisome proliferator-activated receptor alpha (PPARa), which resides in liver and muscle, coordinating lipoprotein and fatty acid homeostasis [1]. Cholesterol homeostasis is dictated by the liver X receptor alpha (LXRa), targeting genes pertaining to the kidney, intestine, liver and adipose tissues [2]. A common partner receptor to PPARa and LXRa is known …
The Use Of Doublecortin To Quantify The Effects Of Pharmacological Treatment On Neurogenesis And Functional Recovery After Stroke, Amber Lee Hensley
The Use Of Doublecortin To Quantify The Effects Of Pharmacological Treatment On Neurogenesis And Functional Recovery After Stroke, Amber Lee Hensley
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Ischemic strokes account for 87% of all strokes and can have debilitating effects on language, sensory, and motor skills. Currently, tPA is the only medication approved by the FDA for the treatment of ischemic stroke, but the window of time to administer the drug is very small. In this thesis, we investigate the use of a simvastatin and fluoxetine drug combination (FS) as a possible treatment for ischemic stroke victims. To analyze the effects of FS on neurogenesis and functional recovery, we utilize the Montoya Staircase and quantify the amount of neurogenesis using doublecortin. Although the results of this study …
Examining Infarct Sizes In Female Sprague Dawley Rats In Response To A Delayed Post-Stroke Pharmacological Treatment In Combination With Physical Rehabilitation, Sayali Ravindra Dharmadhikari
Examining Infarct Sizes In Female Sprague Dawley Rats In Response To A Delayed Post-Stroke Pharmacological Treatment In Combination With Physical Rehabilitation, Sayali Ravindra Dharmadhikari
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In this study, we hypothesize that a pharmacological drug treatment comprised of Fluoxetine, Simvastatin and Ascorbic acid together with physical rehabilitation would reduce infarct sizes. Over the period of 60 days after stroke-induction, 13 of the 23 rats were administered the drugs beginning 20-26 hours after stroke-induction and the rest were assigned to the control group. Physical rehabilitation exercises were initiated from poststroke day 8 and continued for 23 alternate days. The rats were tested for functional recovery using Montoya staircase apparatus and were euthanized after post-stroke day 60. The brains sections were analyzed using Nissl stain for infarct volume …
Metabolism Of Herpes Simplex Virus 1 Infected Raw 264.7 Macrophages, Mary K. Jenkins
Metabolism Of Herpes Simplex Virus 1 Infected Raw 264.7 Macrophages, Mary K. Jenkins
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Macrophages are immune cells that phagocytize pathogens and act as antigen presenting cells. Macrophages are critical in regulating the adaptive immune response and play a key role in neutralizing infection. Macrophages exhibit a variety of phenotypes which have different energy requirements. In the case of HSV-1 infection the virus has been shown to alter metabolic processes of its host. Utilizing a XF24 Extracellular Flux Analyzer the metabolic activity of M0, M1, and M2 (IL-4, IL-13, and IL-10) RAW 264.7 macrophages was quantified for both uninfected cells and in response to HSV-1 infection. The analysis showed uninfected M0 and M2 (IL-4, …
Instability At Trinucleotide Repeat Dnas, Rujuta Yashodhan Gadgil
Instability At Trinucleotide Repeat Dnas, Rujuta Yashodhan Gadgil
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Trinucleotide repeats (TNRs) are sequences prone to formation of non-B DNA structures and mutations; undergo expansions in vivo to cause various inherited neurodegenerative diseases. Hairpin structures formed during DNA replication or repair can cause replication fork stalling and if left unrepaired could cause single or double strand DNA breaks. To test and study this hypothesis we have devised a novel two color marker gene assay to detect DNA breaks at TNRs. By inducing replication stress our results show that TNRs are prone to DNA strand breaks and it is dependent on the repeat tract length. Double strand breaks at structured …
Cross Sensitization Of Depressive-Like Behavior Through Two Depression Related Paradigms: Maternal Separation And Its Effect On The Forced Swim Test In The Guinea Pig, Amanda Danielle Schreibeis
Cross Sensitization Of Depressive-Like Behavior Through Two Depression Related Paradigms: Maternal Separation And Its Effect On The Forced Swim Test In The Guinea Pig, Amanda Danielle Schreibeis
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Separation and Its Effect on the Forced Swim Test In the Guinea Pig Early-life stress such as parental neglect, absence, or abandonment, has been hypothesized to increase the susceptibility for developing depression later in life via sensitization of stress-responsive physiological systems (e.g., pro-inflammatory cytokines, hypothalamic-pituitary-adrenal axis). Guinea pigs offer a potential model, but study has been limited to behavioral observations obtained during maternal separation tests. This thesis examined the generalization of this response by asking whether it would cross-sensitize to behavior in another depressive-related paradigm, the forced swim test. In three experiments, pups underwent three forced swim trials, in shallower …
Fission Yeast As A Model Organism For Fus-Dependent Ycytotoxicity In Amyotrophic Lateral Sclerosis, Alan J. Cone
Fission Yeast As A Model Organism For Fus-Dependent Ycytotoxicity In Amyotrophic Lateral Sclerosis, Alan J. Cone
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Amyotrophic Lateral Sclerosis (ALS) is a neurodegenerative motor neuron disease that causes progressive paralysis and death by asphyxiation. There is no cure or effective treatment; however, previous research has identified several genes that appear related to the pathology of ALS. When mutated, these genes result in proteins that gain toxic functions and disrupt normal cellular processes. Fused in Sarcoma (hFUS) is a human transcription factor in the nucleus that binds to DNA and RNA. Mutations in hFUS are associated with both familial and sporadic cases of ALS, frontotemporal lobar degeneration (FTLD), and cancer. In ALS and FTLD, hFUS is mislocalized …
The Expression Of Dopamine-Related Genes And Behavioral Performance In Mice, Victoria Lynne Dershem
The Expression Of Dopamine-Related Genes And Behavioral Performance In Mice, Victoria Lynne Dershem
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While the neurotransmitter dopamine has been well-studied for its role in mood regulation and activation of the intrinsic reward pathway, several psychiatric disorders linked to dopamine are also known to cause memory impairment, a phenomenon which has attracted much less attention. In the current study, whole-transcriptome RNA sequencing was performed, and transcript levels of several dopamine-related genes were compared to results of behavioral assays designed to test spatial and emotional memory, as well as anxiety. The results suggest a positive relationship between expression level of Nurr1, a nuclear receptor known to initiate transcription of genes necessary for dopaminergic signaling, …
Genetic Analysis Of Male-Specific Lethality Between Caenorhabditis Briggsae:: Caenorhabditis Nigoni F1 Hybrids, Vaishnavi Ragavapuram
Genetic Analysis Of Male-Specific Lethality Between Caenorhabditis Briggsae:: Caenorhabditis Nigoni F1 Hybrids, Vaishnavi Ragavapuram
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Speciation occurs when there is a lack of reproduction due to genetic barriers. These genetic barriers to gene flow are referred as reproductive isolation mechanisms. Pre-zygotic and post-zygotic isolation are types of reproductive isolation mechanisms. In this project, post-zygotic isolation mechanisms were examined. Haldane's Rule states that in F1 hybrids, individuals of the heterogametic sex are less fit than those of the homogametic sex. Darwin's Corollary to Haldane's rule states that there is asymmetry in hybrid progeny between interspecific reciprocal crosses. Crosses done between Caenorhabditis briggsae males to Caenorhabditis nigoni females produce viable F1 hybrid females and males, yet the …
Genomic Signatures Of Population History In A Pair Of Recently Diverged Australian Teal Support Strong Selection On The Z Sex-Chromosome, Kevin Kyle Hawkins
Genomic Signatures Of Population History In A Pair Of Recently Diverged Australian Teal Support Strong Selection On The Z Sex-Chromosome, Kevin Kyle Hawkins
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Sex chromosomes are thought to be an important component of the genome associated with speciation and the buildup of reproductive isolation. Recent advances in sequencing technologies and improvements in population genetics and modeling techniques have made it possible to better assess genomic signatures of selection, genetic drift and gene flow in diverging lineages. Recent studies have shown elevated differentiation on the Z sex-chromosome between the Australian grey teal (Anas gracilis) and chestnut teal (Anas castanea). Here, we used next generation sequencing to scan ~3,400 autosomal loci and ~190 Z loci to examine genomic differentiation and signatures of selection and gene …
Novel Insight Into The Role Of Lxrα In Metabolic Regulation Via Dna Binding As A Heterodimer With Pparα And As A Homodimer, Andrea M. Klingler
Novel Insight Into The Role Of Lxrα In Metabolic Regulation Via Dna Binding As A Heterodimer With Pparα And As A Homodimer, Andrea M. Klingler
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Liver X receptor a (LXRa) plays a critical role in the maintenance of energy homeostasis within a cell through tight transcriptional regulation of genes involved in metabolism of lipids, glucose, and cholesterol. Although LXRa has been established to function as a heterodimer with the retinoid X receptor a (RXRa), recent studies have determined that LXRa also interacts directly with peroxisome proliferator-activated receptor a (PPARa). However, little is known regarding the functionality of this heterodimer, if any exists at all. This study determined that a heterodimer of PPARa and LXRa is capable of binding to candidate response elements in vitro with …
Cell Viability, Cytoskeleton Organization And Cytokines Secretion Of Raw 264.7 Macrophages Exposed To Gram-Negative Bacterial Components, Ali Awadh Alshehri
Cell Viability, Cytoskeleton Organization And Cytokines Secretion Of Raw 264.7 Macrophages Exposed To Gram-Negative Bacterial Components, Ali Awadh Alshehri
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Macrophages play an important role in innate immunity by controlling cellular responses. In this study, the effects of gram-negative bacterial components (Flagellin, lipoprotein, lipopolysaccharides (LPS), outer membrane proteins-A (OMP-A) and peptidoglycan) were determined on cell viability, morphology, cytoskeletal filament and cytokines secretion of murine RAW 264.7 macrophages at 24 hours. The effect of LPS, flagellin and peptidoglycan from gram negative bacteria on viability murine RAW 264.7 macrophages were evaluated using different concentrations (1, 5 and 10 µg/ml). Cells stimulated with LPS displayed ~ 2-fold decrease (P=0.001) in cell viability compared to control cells at 24 hours whereas cells stimulated with …
Correlating Innate Functional Recovery From Stroke Either With Stem Cell Proliferation And/Or Limb Rehabilitation, Devipriyanka Nagarajan
Correlating Innate Functional Recovery From Stroke Either With Stem Cell Proliferation And/Or Limb Rehabilitation, Devipriyanka Nagarajan
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In the present study 10-12 month female rats were examined for functional recovery from stroke and this recovery was compared with the stem cell/progenitor cell proliferation in the brain (which was measured by Ki67). The cell proliferation indicated by Ki67 showed a 6 fold increase in control animals compared to the rehabilitation animals. The contralateral functional recovery in control animals were 46.6% and in the rehabilitation animals were 24.5%. The physical rehabilitation was carried out to determine if limb rehabilitation can promote greater functional recovery. The results showed that when the animals were made to over use their impaired limb …
Chemosensitivity In Mealworms And Darkling Beetles (Tenebrio Molitor) Across Oxygen And Carbon Dioxide Gradients, Andrew King Patterson
Chemosensitivity In Mealworms And Darkling Beetles (Tenebrio Molitor) Across Oxygen And Carbon Dioxide Gradients, Andrew King Patterson
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Breathing in most insects is controlled through a negative feedback loop consisting of signals (O2, CO2, pH), sensors (chemoreceptors), integrators (neural ganglia), and effectors (spiracles over tracheae). I hypothesized that mealworms and their adult counterparts Darkling beetles, Tenebrio molitor, can sense anoxic and hyperoxic environments and preferentially avoid these environments. I also hypothesize that mealworms are attracted to hypercarbia while Darkling beetles avoid hypercarbia. I constructed a test arena to create an O2 or CO2 gradient. Velocity, total distance traveled, and time spent in each area of the O2 or CO2 gradients …
The Expression Of Major Histocompatibility Class I And Major Histocompatibility Class Ii On Macrophages In The Presence Of Aryl Hydrocarbon Antagonist (Ch-223191), Caitlin Wilson
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Macrophages are crucial for ridding the body of debris and foreign cells. The aryl hydrocarbon receptor (AhR) also plays a critical role in immunity. This study examined the effect of the AhR on the expression of major histocompatiability complex class I (MHCI) and MHC class II (MHCII) in two murine macrophage cell lines. This study used Raw264.7 and J774A.1 murine macrophage cell lines. The Raw264.7 cells are from male BALB/c mice while the J774A.1 cells are from female BALB/cN mice. The addition of the AhR anatagonist CH-223191 (AhRa) showed that the AhR does not significantly impact MHCI expression. However, MHCII …
The Effects Of Various Laundering Factors On The Recoverability Of Dna, Erin L. Houston
The Effects Of Various Laundering Factors On The Recoverability Of Dna, Erin L. Houston
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Criminals have been documented to launder clothing in an attempt to hide evidence; however, there limited studies on this type of evidence manipulation. This study looked at: 1) the effects of eighteen laundry additives at diluted and undiluted strengths on human blood, 2) the effects of a delay between deposit and laundering, 3) the amount of recoverable DNA on laundered clothing with different deposited volumes of blood, and 4) the transfer of genetic material within a primary load and between primary/secondary and primary/tertiary loads. There was a reduction in volume of DNA for some laundry additives. Nevertheless, all genotyped samples …
The Anti-Apoptotic Effect Of Hsv-1 On Murine Macrophages: Raw 246.7 Murine Macrophage Cell Line, Mofeda Abdussalam Alhanghari
The Anti-Apoptotic Effect Of Hsv-1 On Murine Macrophages: Raw 246.7 Murine Macrophage Cell Line, Mofeda Abdussalam Alhanghari
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Herpes simplex virus type 1 (HSV-1) is a worldwide pathogen that affects humans and has the ability to establish a latent state of infection in the sensory nerve ganglia after primary infection of epithelial cells (Boutell and Everett, 2003). HSV-1 is a very contagious virus, which can be transmitted from person to person and cause cold sores in the infected person. Rarely, infection can lead to more serious complications, such as encephalitis. Most HSV-1 infections usually occur in childhood with lifelong potential for symptomatic or asymptomatic viral shedding episodes (Looker et al., 2015). HSV- 1 infects 60%-80% of people throughout …
The Development And Validation Of A One Tier Diagnostic Assessment To Test Premedical Students' Misconceptions About Traumatic Brain Injury, Md Hasan Iqbal
The Development And Validation Of A One Tier Diagnostic Assessment To Test Premedical Students' Misconceptions About Traumatic Brain Injury, Md Hasan Iqbal
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Since brain injury is common in the United States, it is important for health professionals and the public to have accurate knowledge about traumatic brain injury (TBI). Understanding misconceptions is important for health educators, nurses, and physicians, who work with TBI patients. While previous studies on misconceptions about TBI have been undertaken, these have not focused on pre-medical students, nor utilized validated assessments. The purpose of this study was to develop and validate a one tier diagnostic test with a confidence index to determine premedical students' misconceptions about TBI. Using the theoretical framework suggested by Treagust (1986, 1988, and 1995), …
Functional Characterization Of Cancer-Related Mutations Of Erk3, Hadel Mohammed Alsaran
Functional Characterization Of Cancer-Related Mutations Of Erk3, Hadel Mohammed Alsaran
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Extracellular signal-regulated kinase 3 (ERK3) is an atypical member of the mitogen-activated protein kinase (MAPK) family. Recent studies have shown that ERK3 is highly upregulated in multiple cancers, such as lung cancer and colon cancer. Importantly, ERK3 promotes cancer cell migration and invasion by phosphorylating steroid receptor activator 3 (SRC-3), hence upregulating pro-invasive matrix metalloproteinase genes. While the link between ERK3 and cancers has been recognized, little is known about ERK3 mutations in cancer progression. In this study, we have investigated ERK3 mutations on arginine 64 (arginine 64 mutated to cysteine or histidine, R64C or R64H) and leucine 290 (leucine …
Evidence Of Morphological Variation Between Bluegill Lepomis Macrochirus Rafinesque, 1819 Populations Across Grand Lake St. Mary’S Watershed, Anthony Jerome Bell
Evidence Of Morphological Variation Between Bluegill Lepomis Macrochirus Rafinesque, 1819 Populations Across Grand Lake St. Mary’S Watershed, Anthony Jerome Bell
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A myriad of factors have been shown to affect the morphology of freshwater fish. Despite the growing base of ecomorphology literature there is little information available exploring how these factors relate to body morphology of Centrarchidae, specifically in the Lepomis genera. Therefore, the objective of this study was to describe variation in body morphology of Bluegill (Lepomis macrochirus) across Grand Lake St. Mary’s watershed area and test for covariation of morphology with size, sex, and habitat. Geometric morphometric methods were used to assess variation among individuals and general linear models were used to test for covariation of morphology with size, …
The Expression Of Aryl Hydrocarbon Receptor In Raw 264.7 Macrophages In The Presence Of Socs1 Peptide And Socs3 Peptide Mimetic And Cells Infected With Hsv-1, Maher Salem Alwethaynani
The Expression Of Aryl Hydrocarbon Receptor In Raw 264.7 Macrophages In The Presence Of Socs1 Peptide And Socs3 Peptide Mimetic And Cells Infected With Hsv-1, Maher Salem Alwethaynani
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Macrophages play a crucial role for our immune system and protect our body from infection. Suppressor of cytokine signaling (SOCS) proteins negatively regulate cytokine receptor and TLRs. The aryl hydrocarbon receptor (AhR) also performs an important role in immunity. This study investigated the changes in expression of AhR in RAW 264.7 macrophage cells after the addition of SOCS1 and SOCS3 peptide mimetics and also examined AhR expression in RAW 264.7 macrophage cells before and after the addition of HSV-1 RAW 264.7 murine macrophage cell lines which are from male BALB/c mice were used in this study. The addition of the …
Biomimetic Production Techniques For Mechanical And Chemical Characterization Of Sucker Ring Teeth Isoform-12 From The Dosidicus Gigas Squid, Marcus T. Grant
Biomimetic Production Techniques For Mechanical And Chemical Characterization Of Sucker Ring Teeth Isoform-12 From The Dosidicus Gigas Squid, Marcus T. Grant
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The unique protein-based structure of Sucker Ring Teeth (SRT) of cephalopods have spurned research into the molecular design, physical characteristics, functionality and mechanical properties to explore biomimetic engineering and biochemical potential for eventual industrial production. Previous research has elucidated the potential for scientific and industrial exploitation. However, much of the previous research focused on the most abundant protein isoform of the sucker ring teeth, suckerin-19 (also known as suckerin-39) from the Jumbo or Humboldt Squid (Dosidicus Gigas). There is little known about the characteristics of the other 37 protein isoforms of Sucker Ring Teeth. Although the other isoforms have similar …
Determining Protein-Protein Interactions Of Als-Associated Sod1, Leah A. Shurte
Determining Protein-Protein Interactions Of Als-Associated Sod1, Leah A. Shurte
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Amyotrophic Lateral Sclerosis (ALS) is a neurodegenerative disorder that occurs due to the death of motor neurons and leads to paralysis and death within three to five years after symptoms present (Byrne et al., 2013). Superoxide Dismutase 1 (SOD1) was first identified to be associated with ALS in 1993. The objective of this study is to determine which proteins interact with wild type and mutant SOD1 and find any similarities or differences between them. ALS is attributed to a gain of toxicity, therefore abnormal protein interactions in mutant SOD1 are important. The results of this study will provide insight on …
Sk Channel Clustering In Sod1-G93a Motoneurons, Saihari Shekar Dukkipati
Sk Channel Clustering In Sod1-G93a Motoneurons, Saihari Shekar Dukkipati
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Amyotrophic lateral sclerosis (ALS) is a devastating neuromuscular disease that currently has no cure and extremely limited treatment options. The specific mechanisms that underlie motoneuron degeneration and death, which are classical features of this disease, are mostly unknown. This thesis tests the hypothesis that small-conductance calcium-activated potassium channels (SK) may be downregulated in ALS motoneurons, as suggested by computational modelling. SK channel expression was measured in spinal alpha-motoneuron cell bodies or somata of wildtype (WT) and mutant (mt) SOD1-G93A mice, a transgenic animal model of ALS. Quantitative immunohistochemical analysis of the developmental expression of SK channel isoforms SK2 and SK3 …
Identification Of Protein-Protein Interactions Of Amyotrophic Lateral Sclerosis Associated Protein Tdp-43, Hanoor Sharma
Identification Of Protein-Protein Interactions Of Amyotrophic Lateral Sclerosis Associated Protein Tdp-43, Hanoor Sharma
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Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease caused by progressive degeneration of upper and lower motor neurons in the brain and spinal cord. Multiple mutations are found in some of the proteins associated with ALS, including superoxide dismutase (SOD1), fused in sarcoma (FUS) and trans-activation response DNA-binding protein (TDP-43). TDP-43 is a DNA and RNA binding protein, well conserved, and ubiquitously expressed in all tissues. TDP-43 resides in the nucleus and sometimes shuttles between nucleus and cytoplasm. Mutations in TDP-43 leads to mislocalization of TDP-43 to the cytosol where it was ubiqutinated and hyperphosphsorylated, ultimately leading to neuronal cell …
Interaction Of Na+/K+ Atpase With Bcl-2 Proteins: Isolated Enzyme Vs Epithelial Cell Extracts, Chandra Kumar Maharjan
Interaction Of Na+/K+ Atpase With Bcl-2 Proteins: Isolated Enzyme Vs Epithelial Cell Extracts, Chandra Kumar Maharjan
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The nearly complete inhibition of Na+/K+ ATPase (NKA) in fetal human lens (FHL) epithelial cells by chelerythrine (CHE), a Bcl-2 homology (BH)3-mimetic quaternary benzophenanthridine alkaloid was proposed to be the consequence of CHE binding at a BH1-like hydrophobic groove at the cytosolic aspect of NKA. This conclusion was based on in silico analysis showing at least two motifs in the N-terminal domain of NKA’s a1 subunit- i) aa 59-71 (ARAAEILARDGPN) and ii) aa 42-48 (DELHRKY) homologous to the BH1 and BH3 motifs of Bcl-2 (B-cell lymphoma) respectively, leading to a novel hypothesis that NKA could interact with these proteins by …
Adeno-Associated Virus-Vegf-165 Mediated Modification Of Adipose Derived Stem Cells For Cell Therapy, Upasana Niyogi
Adeno-Associated Virus-Vegf-165 Mediated Modification Of Adipose Derived Stem Cells For Cell Therapy, Upasana Niyogi
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Chronic wounds have become a major clinical and economic burden in our society. New approaches that accelerate wound healing are desperately needed. Angiogenesis and vascularization play a critical role in healing. One of the essential angiogenic factors that promote the formation of vascular beds is vascular endothelial growth factor (VEGF). Moreover, adipose-derived stem cells (ASC), through their regeneration and differentiation properties, may promote healing when transplanted into a wound bed. I have proposed to develop a novel organotypic wound model to facilitate the study of wound healing process. I hypothesize that administration of genetically-modified ASC secreting VEGF via an adeno-associated …
The Effects Of Cold And Freezing Temperatures On The Blood Brain Barrier And Aquaporin 1, 4, And 9 Expression In Cope's Gray Treefrog (Hyla Chrysoscelis), Dalal Nouruldeen Felemban
The Effects Of Cold And Freezing Temperatures On The Blood Brain Barrier And Aquaporin 1, 4, And 9 Expression In Cope's Gray Treefrog (Hyla Chrysoscelis), Dalal Nouruldeen Felemban
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In sub-freezing temperatures, cerebrospinal fluid (CSF) and blood plasma within the brain of freeze-tolerant gray treefrogs, Hyla chrysoscelis, are likely to freeze. We hypothesized that this would challenge blood-brain barrier (BBB) integrity and volume regulation for cells in the brain. Our first hypothesis, tested as cerebral Evans blue permeability, was not supported; BBB integrity appeared uncompromised by cold. Our second hypothesis, tested as changing expression of aquaporin (AQP) proteins, was partially supported. Whole-brain expression (Western blot) of AQP1 and AQP4 were unchanged by cold conditions, but AQP9 expression increased in thawed animals. AQP1 was found (immunohistochemistry) in ependymal cells, endothelial …