Open Access. Powered by Scholars. Published by Universities.®
- Keyword
-
- Pharmacology (30)
- Toxicology (17)
- ACE2 (6)
- Diabetes (6)
- Sarin (5)
-
- TCDD (5)
- Cytokines (4)
- Keratinocytes (4)
- Medicine (4)
- Molecular Biology (4)
- RAS (4)
- Biomedical Research (3)
- Collagen (3)
- Growth Factors (3)
- Hydroxyurea (3)
- IGF-1 (3)
- Mass Spectrometry (3)
- Microvesicle Particles (3)
- Organophosphate (3)
- Platelet Activating Factor (3)
- Silver Nanoparticles (3)
- Skin cancer (3)
- Stroke (3)
- 8-OH-DPAT (2)
- ACE (2)
- ATR inhibitor (2)
- ATR kinase (2)
- Acid Sphingomyelinase (2)
- Acid sphingomyelinase (2)
Articles 1 - 30 of 97
Full-Text Articles in Life Sciences
Heightened Levels Of Microvesicle Particles Resulting From Combination Of Ethanol And Thermal Burn Injury, Chad Alan Brewer
Heightened Levels Of Microvesicle Particles Resulting From Combination Of Ethanol And Thermal Burn Injury, Chad Alan Brewer
Browse all Theses and Dissertations
Ethanol (EtOH), in combination with thermal burn injury, is a clinically significant problem resulting in an increase in morbidity and mortality due to acute multi-organ toxicity from excess systemic cytokine release. This intoxicated thermal burn affects close to 50% of the total numbers of hospital-admitted burn patients and currently has no standard treatment. However, using in vitro cellular and in vivo murine models, our group has provided data implicating the augmented production of the lipid mediator Platelet-activating factor (PAF) in keratinocytes in response to intoxicated thermal burn injury in the subsequent pathology. Of importance, our group has demonstrated that activation …
Replication Stress Induced By The Ribonucleotide Reductase Inhibitors Guanazole, Triapine, And Gemcitabine In Fission Yeast, Mashael Yahya A Alyahya
Replication Stress Induced By The Ribonucleotide Reductase Inhibitors Guanazole, Triapine, And Gemcitabine In Fission Yeast, Mashael Yahya A Alyahya
Browse all Theses and Dissertations
Replication stress can be produced by various exogenous or endogenous factors that perturb the movement of replication forks. To overcome the stress, the cells provoke the DNA replication checkpoint to mobilize several pathways to protect the forks, maintain genome integrity, and promote cell survival. Defects in the replication checkpoint cause forks collapse, leading to chromosomal DNA damage or cell death. Although the replication checkpoint is crucial for genome integrity in all eukaryotes, the underlying mechanisms remains to be fully understood. To investigate the mechanisms of DRC, hydroxyurea (HU), an established inhibitor of ribonucleotide reductase (RNR), has been widely used in …
Impaired Β-Cell Neogenesis In A Mouse Model Of Metabolic Syndrome, Modhi Abdullah Alshammari
Impaired Β-Cell Neogenesis In A Mouse Model Of Metabolic Syndrome, Modhi Abdullah Alshammari
Browse all Theses and Dissertations
Pancreatic islet β-cell plays an essential role in insulin release and hence glucose homeostasis. The maintenance of glucose homeostasis depends on β-cell ability to cope with enough insulin to fulfill metabolic and physiological demands. Adequate insulin release is the result of highly complex and dynamic interplays between acute changes in β- cell electrical activity, exocytosis and chronic adaptations in cellular function, volume, mass and proliferation. All of this appears modulated, to some extent, by the functional presence of Slc12a2 symporters, also known as Na+K+2Cl– cotransporter-1 (Nkcc1), which accumulates intracellular Cl– above its electrochemical equilibrium. Recent studies from our laboratory showed …
Ahr-Mediated Transcriptional Regulation Of The Human Immunoglobulin Hs1.2 Enhancer, Sydney White
Ahr-Mediated Transcriptional Regulation Of The Human Immunoglobulin Hs1.2 Enhancer, Sydney White
Browse all Theses and Dissertations
The 3'IGHRR is thought to be responsible for the transcription of the immunoglobulin heavy chain (IGH) gene locus, which is essential for the production of antibodies. The human 3’IGHRR, which is duplicated in humans, contains the hs3, hs4, and hs1.2 enhancers. Additionally, the hs1.2 enhancer is polymorphic in humans and consists of a 53 bp invariant sequence that can be repeated one to four times. Previous experiments in a mouse and human B-cell line model have shown that the high affinity AhR ligand and environmental toxicant 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) can induce hs1.2 enhancer activity in an AhR-dependent manner. The AhR is …
The Effect Of Circadian Clock Modulation On Cisplatin Cytotoxicity, Nadeen Nibal Ahmad Anabtawi
The Effect Of Circadian Clock Modulation On Cisplatin Cytotoxicity, Nadeen Nibal Ahmad Anabtawi
Browse all Theses and Dissertations
Cisplatin is a DNA damage-based chemotherapeutic drug widely used to treat various types of cancers; however, the treatment's toxicity restricts its efficiency. Studies have shown that the circadian rhythm controls the DNA damage response and affects the repair pathways of cisplatin-induced DNA damage. Circadian clock modulation, therefore, has been proposed to be a potential mechanism for enhancing cisplatin tolerability. Here we used clock-enhancing molecules to evaluate the effect of pharmacological clock modulation on cisplatin cytotoxicity. Using cultured human cell lines, cisplatin cytotoxicity was found to be attenuated following treatment with circadian-enhancing molecules KS15 and SR8278. Moreover, the protein and mRNA …
Stress-Induced Senescence In Human Dermal Fibroblasts : Effects Of Creatine And Nicotinamide Post Stress Treatment, Venkata Sravya Arikatla
Stress-Induced Senescence In Human Dermal Fibroblasts : Effects Of Creatine And Nicotinamide Post Stress Treatment, Venkata Sravya Arikatla
Browse all Theses and Dissertations
In skin connective tissue, dermal fibroblasts appear to be the most prevalent cell type. They are in charge of making the extracellular matrix that makes up the skin's connective tissue, and also involved in wound healing. Moreover, they produce Insulin-like growth factor-1 (IGF-1) which helps in activation of Insulin growth factor-1 receptor (IGF-1R). This receptor helps to control cell proliferation and responses to DNA-damaging substances such as UVB radiation, reactive oxygen species (ROS), and therapeutic drugs. According to our findings, lack of IGF-1 expression in the dermis of elderly patients due to fibroblast senescence (senescence is characterized by which cells …
The Protective Effects Of Mir-210 Modified Endothelial Progenitor Cells Released Exosomes In Hypoxia/Reoxygenation Injured Neurons, Sri Meghana Yerrapragada
The Protective Effects Of Mir-210 Modified Endothelial Progenitor Cells Released Exosomes In Hypoxia/Reoxygenation Injured Neurons, Sri Meghana Yerrapragada
Browse all Theses and Dissertations
Endothelial progenitor cells (EPCs) have been shown to provide beneficial effects on oxidative stress. Exosomes (EXs) released from these stem cells could be one of the major contributors, as they are known to convey the benefit of one cell to another cell via microRNAs (miRNA). At first, we determined that EPCs release more EXs when they are serum-starved for 48 hours., and by determining the microRNA-210 (miR-210) levels in the EXs, we found that miRNA is being transferred from cells to EXs. Meanwhile, miR-210 is gaining popularity in reducing elevated oxidative stress levels. In this study, we investigated the role …
Functions Of Atr Kinase In Terminally Differentiated Human Epidermal Keratinocyles And In Human Ex-Vivo Skin After Exposure To Ultraviolet B Radiation, Vivek Shashank Nag Gogusetti
Functions Of Atr Kinase In Terminally Differentiated Human Epidermal Keratinocyles And In Human Ex-Vivo Skin After Exposure To Ultraviolet B Radiation, Vivek Shashank Nag Gogusetti
Browse all Theses and Dissertations
The functions of Ataxia telangiectasia and Rad-3 related protein (ATR) is very much important in a cell, as it is a DNA damage response protein, which plays an important role in cell division, DNA repair and apoptosis. This protein helps in proliferation in the actively DNA dividing normal cells and in cancer cells. The functions of ATR in a proliferating cell are well studied and known to involve regulation of replication fork and cell cycle progression after DNA damage. Whereas, in a non-replicating cell, the functions of ATR are not so well known. In the human body, most of the …
Xeroderma Pigmentosum A Deficiency Results In Increased Generation Of Microvesicle Particles In Response To Ultraviolet B Radiation, Christian Lea Rajeshkumar
Xeroderma Pigmentosum A Deficiency Results In Increased Generation Of Microvesicle Particles In Response To Ultraviolet B Radiation, Christian Lea Rajeshkumar
Browse all Theses and Dissertations
Xeroderma Pigmentosum is a genetic disorder in which ability to repair DNA damage such as from UV radiation is decreased. Nucleotide excision repair is known for repairing DNA damage caused by UV radiation and XPA plays a major role in recognizing and eliminating abnormal section of DNA. Therefore, XPA deficiency decreases repair efficiency of DNA. Of note, XPA deficiency is linked with photosensitivity. Microvesicle particles are membrane-bound particles which are released into the extracellular environment in response to multiple stimuli including the lipid Platelet activating factor (PAF). Previous studies have shown that XPA deficiency can induce increase production of reactive …
Insulin-Like Growth Factor-1 (Igf-1) Impacts P53-Regulated Gene Products In Uvb-Irradiated Human Keratinocytes And Skin Epidermis, Abdulrhaman Mohammed Mohammed Alkawwar
Insulin-Like Growth Factor-1 (Igf-1) Impacts P53-Regulated Gene Products In Uvb-Irradiated Human Keratinocytes And Skin Epidermis, Abdulrhaman Mohammed Mohammed Alkawwar
Browse all Theses and Dissertations
Skin cancer is the most prevalent human malignancy and is primarily caused by ultraviolet (UV) wavelengths of sunlight. However, the fact that most skin cancers occur in people over the age of 60 indicates that advantaged age is a second skin cancer risk factor. Why geriatric skin is prone to developing skin cancers is not clear, but several studies have shown that dermal fibroblasts in geriatric skin express lower levels of the hormone insulin-like growth factor-1 (IGF-1) than young adult skin and that deficient IGF-1 signaling negatively impacts how epidermal keratinocytes respond to UVB radiation. A major regulator of the …
Identification Of New Metabolic Mutations In The Fission Yeast Schizosaccharomyces Pombe That Sensitize The Cell To Hydroxyurea, Alaa Mahdi
Browse all Theses and Dissertations
Cancer is a life-threatening illness and innovative research is therefore required to fuel the development of new anti-cancer therapies. As an anti-proliferative drug, hydroxyurea (HU) has been used in the treatment of various neoplastic and non-neoplastic diseases such as sickle cell anemia, psoriasis, and viral infections. HU is a well-known inhibitor of ribonucleotide reductase (RNR), an enzyme that generates dNTPs for DNA replication and repair. In our genetic screen in fission yeast looking for mutants with defects in checkpoint response, we also found a set of mutants that are highly sensitive to HU but with a functional checkpoint response. It …
Multiple Genotoxic Agents Activate Atr Kinase Signaling In Quiescent Human Cells, Mariyyah Ahmed O. Madkhali
Multiple Genotoxic Agents Activate Atr Kinase Signaling In Quiescent Human Cells, Mariyyah Ahmed O. Madkhali
Browse all Theses and Dissertations
The ATR protein kinase is activated in response to DNA damage and other forms of genotoxic stress caused by both environmental carcinogens and anti-cancer drugs. However, much of our understanding of ATR kinase function is limited to proliferating cells in which DNA replication stress is the primary signal for ATR activation and where the major regulatory targets of ATR signaling are proteins involved in DNA synthesis and cell cycle progression. Here we have used HaCaT keratinocytes maintained in a non-replicating, quiescent state in vitro to examine how cell killing by different genotoxic agents is impacted by cell growth status and …
Topical Photodynamic Therapy Generates Microvesicle Particles, Oladayo Ayobami Oyebanji
Topical Photodynamic Therapy Generates Microvesicle Particles, Oladayo Ayobami Oyebanji
Browse all Theses and Dissertations
Photodynamic therapy (PDT) involves the use of light at an appropriate wavelength acting on a photosensitizing chemical to cause cell death via generation of reactive oxygen species. PDT has been useful in the management of skin conditions (like acne, psoriasis) and cancers like superficial skin, esophageal and non-small cell lung cancers. In addition to these therapeutic effects, previous murine studies from our group have demonstrated that topical PDT induces immunosuppression in vivo. Thus, topical PDT of skin can generate systemic effects through unknown mechanisms. Our group showed that PDT induces an immunosuppressive effect which occurs partly via Platelet-Activating Factor Receptor …
Role Of Exercise In Macrophage Polarization Of Perivascular Adipose Tissue And Adipose Tissue Inflammation In Hypertensive Mice Model, Venkata Sai Usha Sri Polaki
Role Of Exercise In Macrophage Polarization Of Perivascular Adipose Tissue And Adipose Tissue Inflammation In Hypertensive Mice Model, Venkata Sai Usha Sri Polaki
Browse all Theses and Dissertations
Hypertension, also known as high blood pressure, is a long-term medical condition in which the blood pressure in the arteries is persistently elevated. It can lead to severe health complications and increase the risk of heart disease, stroke, and sometimes death. Perivascular adipose tissue (PVAT) is known as the adipose tissue (AT) surrounding all the blood vessels and plays a critical role in the pathogenesis of the vascular disease. In vascular pathologies, PVAT increases in volume and becomes dysfunctional, with altered cellular composition and molecular characteristics. Macrophages have been found to accumulate in PVAT during hypertension, which might be involved …
Effects Of Creatine And Nicotinamide On Experimentally Induced Senescence In Dermal Fibroblasts, Avinash Satyanarayan Mahajan
Effects Of Creatine And Nicotinamide On Experimentally Induced Senescence In Dermal Fibroblasts, Avinash Satyanarayan Mahajan
Browse all Theses and Dissertations
Dermal fibroblasts provide structural support by producing collagen and other structural/support proteins beneath the epidermis. Fibroblasts also produce Insulin-like growth factor-1 (IGF-1), which binds to the IGF-1 receptors (IGF-1Rs) on keratinocytes to activate signaling pathways that regulate cell proliferation and cellular responses to genotoxic stressors like ultraviolet B radiation found in sunlight. Our group has determined that the lack of IGF-1 expression due to fibroblast senescence in the dermis of geriatric individuals is correlated with an increased incidence of skin cancer in geriatric patients. The present studies were designed to test the hypothesis that pro-energetics like creatine monohydrate and nicotinamide …
The Feeding Pattern Of C57bl/6j Mice, Yakshkumar Dilipbhai Rathod
The Feeding Pattern Of C57bl/6j Mice, Yakshkumar Dilipbhai Rathod
Browse all Theses and Dissertations
The feeding pattern and control of energy intake in social mice are poorly understood. Here, we determined and quantify the normal nocturnal feeding pattern of group-housed male and female mice of the C57BL/6J genetic background fed a chow diet. Mice at 10 and 20weeks of age showed the expected age-dependent increase in lean and fat mass being them more pronounced in males. Under ad libitum conditions, male and female mice ate comparable amounts of food per unit of body weight irrespective of gender. The analysis of the feeding microstructure revealed that energy intake in females is the result of significantly …
Differing Functions Of Atr Kinase In Human Epidermal Keratinocytes Exposed To Ultraviolet B Radiation, Kavya Shaj
Differing Functions Of Atr Kinase In Human Epidermal Keratinocytes Exposed To Ultraviolet B Radiation, Kavya Shaj
Browse all Theses and Dissertations
Ataxia-telangiectasia and Rad-3 related (ATR) is a DNA damage response protein kinase that is upregulated in various cancer cell types and has key roles in cell cycle progression, DNA repair and apoptosis. Small molecule ATR kinase inhibitors are of importance as antineoplastic agents because they sensitize cancer cells to DNA damaging chemotherapy drugs. However, only a few studies have looked at the effects of ATR kinase inhibitors on non-replicating cells, which constitute the majority of cells in the human body and have suggested an opposite, pro-apoptotic role for ATR kinase signaling in non-replicating cells in comparison to a pro-survival function …
Kinetics Of Microvesicle Particle Release In Keratinocytes, Pariksha Thapa
Kinetics Of Microvesicle Particle Release In Keratinocytes, Pariksha Thapa
Browse all Theses and Dissertations
Microvesicle particles (MVPs) are subcellular particles that could be involved in inter-cellular communication because they carry various bioactive substances including cytokines. Previous studies from our lab has shown that that the lipid mediator Platelet-activating Factor (1-alkyl-2-acetyl-glycerophosphocholine; PAF) and ultraviolet B radiation (UVB) enhances the release of MVP in various cell types like primary keratinocytes, epithelial cell lines, and murine skin. We hypothesized that there may be synergistic increases in MVP release after combination of treatment of keratinocytes with UVB and PAF agonist (CPAF)/phorbol ester (PMA). The combination treatment significantly increases MVP and cytokine release at 4 to 8 hours time …
Effect Of Endothelial Progenitor Cell-Derived Exosomes On High Glucose And Hypoxia/ Reoxygenation-Induced Injury Of Astrocytes, Manasi Suchit Halurkar
Effect Of Endothelial Progenitor Cell-Derived Exosomes On High Glucose And Hypoxia/ Reoxygenation-Induced Injury Of Astrocytes, Manasi Suchit Halurkar
Browse all Theses and Dissertations
In this study, we tested the protective effects of EPC-EXs (endothelial progenitor cell derived exosomes) and miR-126 EPC-EXs (microRNA-126 EPC-EXs) on the astrocytes injured by HG (high glucose) plus H/R (hypoxia/reoxygenation) model. At first, we determined the concentration and time dependent uptake of EPC-EXs by astrocytes. It was also found that the EPC-EXs were uptaken via macropinocytosis, caveolin-dependent and clathrin-mediated pathways in astrocytes. Furthermore, the astrocyte cell line was injured through the HG + H/R model. EXs, isolated by ultracentrifugation from the EPC culture supernatant were co-incubated with the injured cells. It was found that EPC-EXs and miR-126 EPC-EXs decrease …
Identification Of New Metabolic Mutations That Enhance The Cell-Killing Effect Of Hydroxyurea, A Clinically Used Drug With Multiple Implications, Rittu Elsa Samuel
Identification Of New Metabolic Mutations That Enhance The Cell-Killing Effect Of Hydroxyurea, A Clinically Used Drug With Multiple Implications, Rittu Elsa Samuel
Browse all Theses and Dissertations
Cancer is one of the leading causes of death and a worldwide health issue. Intensive studies have been conducted in the past to unearth new anti-cancer agents. One such anti-proliferative drug is hydroxyurea (HU). HU has been used in clinics for ≥ 100 years to treat various neoplastic and non-neoplastic diseases. Although newer agents have been developed, as a WHO-enlisted essential medicine, it remains the staple drug for the management of chronic myeloproliferative disorders and sickle cell anemia. A better understanding of the HU-induced cell death may improve or expand the therapeutic spectrum of this clinically important drug. HU arrests …
Exosomes Released From Multiple Myeloma Cells Influence The Angiogenic Function Of Endothelial Cells By Regulating Microrna-29b, Qinmao Ye
Browse all Theses and Dissertations
Multiple myeloma is a hematological malignancy characterized by clonal proliferation of plasma cells generally caused by chromosomal abnormalities. It occurs in the bone marrow, which is the microenvironment of multiple myeloma. Exosomes (EXs) are 30-100 nm membrane-derived micro-vesicles containing various of bioactive molecules, such as microRNAs, to mediate the cell-cell interaction. Numerous studies reported that exosomes play a significant role in tumor microenvironment. Angiogenesis has the important implication in tumor exacerbation to supply nutrients to promote the progression of cancer cells through endothelial cells (ECs). Some studies demonstrated that microRNA-29b (miR-29b) can suppress tumor development and inhibit angiogenesis. Therefore, in …
Regulation Of Microvesicle Particle Release In Keratinocytes, Azeezat Afolake Awoyemi
Regulation Of Microvesicle Particle Release In Keratinocytes, Azeezat Afolake Awoyemi
Browse all Theses and Dissertations
Microvesicle particles (MVPs) are produced from cellular membranes and are thought to mediate cell-cell communication, including in response to stressors such as UVB radiation and thermal burn injury. Previous studies have shown that stress-induced MVP release requires the platelet-activating factor (PAF) receptor in human keratinocytes and that pharmacological inhibition of acid sphingomyelinase (ASM) blocked this release. To validate a genetic role for ASM in MVP release, we used CRISPR-Cas9 gene silencing in human keratinocytes and primary fibroblasts derived from ASM-knockout mice. Though MVP release was partially blocked in ASM-deficient mouse fibroblasts, the inability to fully knockdown ASM in HaCaT cells …
Nanomaterial Charge-Dependent Platelet Activating Factor Receptor Agonism In Human Epidermal Cells, Shahryar Jamshed Qureshi
Nanomaterial Charge-Dependent Platelet Activating Factor Receptor Agonism In Human Epidermal Cells, Shahryar Jamshed Qureshi
Browse all Theses and Dissertations
Platelet Activating Factor (PAF) and its associated receptor, the PAF Receptor (PAFR), are important mediators of intercellular communication during an immune response. Once a physiological stimulus triggers an inflammatory response, epithelial, endothelial and immune cells synthesize and release PAF. PAF mediates the recruitment of immune cells, platelets, angiogenesis, expression of various genes, and increased PAF biosynthesis (Brown, 2006; Han, 2006; Whatley, 1988; Axelrod, 1988). In this study, we utilized HaCaT cells and a well characterized KB cell line derived from nasopharyngeal cells, which do not natively express the PAFR. KB cells had previously been transfected with a PAF receptor (KBP) …
Thermal Burn Injury Induced Microvesicle Particle Release, Katherine Erin Fahy
Thermal Burn Injury Induced Microvesicle Particle Release, Katherine Erin Fahy
Browse all Theses and Dissertations
Microvesicle particles (MVP) are found to be important for cellular communication because they contain many bioactive proteins, lipids, cytokines, and nucleic acids. We have previously found that ultraviolet B radiation (UVB) and a Platelet-activating factor agonist (CPAF) can stimulate the release of MVP in keratinocytes. We hypothesized that there may also be an increase in MVP released after thermal burn and that could be involved in pathogenesis of the systemic effects found in some patients. In this thesis various keratinocyte cell lines, mice and human ex vivo skin were used as model systems to test our hypotheses. It was determined …
Extracellular Microvesicles As A Novel Biomarker For Wound Healing, Walid Omran Mari
Extracellular Microvesicles As A Novel Biomarker For Wound Healing, Walid Omran Mari
Browse all Theses and Dissertations
Introduction: Wound healing is a sophisticated dynamic process that involves complex coordination among a variety of resident cells in a suitable extracellular environment. Chronic wounds are defined as wounds that fail to heal within a period of 3 months. Negative pressure wound therapy (NPWT) is a closed suction drainage system is used to enable mass transport of fluid from the body as an adjunct to surgical procedures. (Oasis-ultra) is a triple layer extracellular matrix containing different types of glycosaminoglycans (GAGs) like heparin sulfate, hyaluronic acid and chondroitin sulfate. In addition, it has adhesion molecule such as fibronectin and laminin along …
Effect Of Hyperglycemia And Thiazolidinediones On Cardiac Angiotensin Converting Enzyme 2 (Ace2) And Neprilysin (Nep) In Db/Db Diabetic Mice., Rucha Fadnavis
Effect Of Hyperglycemia And Thiazolidinediones On Cardiac Angiotensin Converting Enzyme 2 (Ace2) And Neprilysin (Nep) In Db/Db Diabetic Mice., Rucha Fadnavis
Browse all Theses and Dissertations
Type 2 diabetes mellitus is strongly associated with increased risk of cardiovascular disease. Despite availability of several antidiabetic medications, the cardiovascular outcomes remain unchanged. Activation of renin angiotensin system is one of the critical factors in development of diabetic complications. Hyperglycemia causes an increase in tissue and circulating Angiotensin II. Angiotensin converting enzyme (ACE2) and neprilysin (NEP) are angiotensin (1-7) forming enzymes. ACE2 was first cloned from heart failure patients and has cardioprotective and renoprotective properties. Combination of angiotensin receptor antagonism and NEP inhibition is a new therapeutic strategy for treatment of heart failure. The aim of this study was …
Effect Of Hyperglycemia And Thiazolidinediones On Cardiac Angiotensin Converting Enzyme 2 (Ace2) And Neprilysin (Nep) In Db/Db Diabetic Mice, Rucha Fadnavis
Effect Of Hyperglycemia And Thiazolidinediones On Cardiac Angiotensin Converting Enzyme 2 (Ace2) And Neprilysin (Nep) In Db/Db Diabetic Mice, Rucha Fadnavis
Browse all Theses and Dissertations
Type 2 diabetes mellitus is strongly associated with increased risk of cardiovascular disease. Despite availability of several antidiabetic medications, the cardiovascular outcomes remain unchanged. Activation of renin angiotensin system is one of the critical factors in development of diabetic complications. Hyperglycemia causes an increase in tissue and circulating Angiotensin II. Angiotensin converting enzyme (ACE2) and neprilysin (NEP) are angiotensin (1-7) forming enzymes. ACE2 was first cloned from heart failure patients and has cardioprotective and renoprotective properties. Combination of angiotensin receptor antagonism and NEP inhibition is a new therapeutic strategy for treatment of heart failure. The aim of this study was …
Usage Of Extracellular Microvesicles As Novel And Promising Therapeutic Tool In Wound Healing, Sami Gamaleddin F. Alsabri
Usage Of Extracellular Microvesicles As Novel And Promising Therapeutic Tool In Wound Healing, Sami Gamaleddin F. Alsabri
Browse all Theses and Dissertations
Introduction Extracellular Microvesicles (EMVs) can carry genetic messages and biologically active proteins throughout tissues and the body. Because of their transport capabilities, EMVs play an important role both diseased and healthy conditions. For example, EMVs play an important regenerative role in many damaged tissues. In the current studies, we examine the role of EMVs in epithelial wound healing. The potential use of EMVs as drug delivery vehicles has gained considerable scientific interest because they can be delivered in circulation, can be targeted to specific areas/cells, and can pass natural barriers. In the current work, we investigate the potential of EMVs …
Effect Of Oasis-Ultra Matrix On The Healing Rate Of Stage Iv Pressure Wounds, Abdelfatah Shaban Abou Issa
Effect Of Oasis-Ultra Matrix On The Healing Rate Of Stage Iv Pressure Wounds, Abdelfatah Shaban Abou Issa
Browse all Theses and Dissertations
Introduction: (Oasis-ultra) is an extra cellular collagen rich matrix derived from porcine intestinal sub-mucosa. A prospective, multi-centered, randomized, single-blinded clinical trial was conducted to study the effects of Oasis-ultra combined with negative pressure wound therapy (NPWT) on the healing rate of stage IV pressure wounds versus NPWT alone. Materials and Methods: Twelve subjects were involved in the study: six patients in the study group and six in the control group. NPWT was changed twice a week for all subjects, and Oasis-ultra was applied weekly. The wounds were measured weekly, and the healing rate was calculated for each subject for 12 …
Examining Infarct Sizes In Female Sprague Dawley Rats In Response To A Delayed Post-Stroke Pharmacological Treatment In Combination With Physical Rehabilitation, Sayali Ravindra Dharmadhikari
Examining Infarct Sizes In Female Sprague Dawley Rats In Response To A Delayed Post-Stroke Pharmacological Treatment In Combination With Physical Rehabilitation, Sayali Ravindra Dharmadhikari
Browse all Theses and Dissertations
In this study, we hypothesize that a pharmacological drug treatment comprised of Fluoxetine, Simvastatin and Ascorbic acid together with physical rehabilitation would reduce infarct sizes. Over the period of 60 days after stroke-induction, 13 of the 23 rats were administered the drugs beginning 20-26 hours after stroke-induction and the rest were assigned to the control group. Physical rehabilitation exercises were initiated from poststroke day 8 and continued for 23 alternate days. The rats were tested for functional recovery using Montoya staircase apparatus and were euthanized after post-stroke day 60. The brains sections were analyzed using Nissl stain for infarct volume …