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Expression Of Inflamatory Response Genes In Ferrets Challenged With H5n1 Avian Influenza Virus, Brock M. Miniard
Expression Of Inflamatory Response Genes In Ferrets Challenged With H5n1 Avian Influenza Virus, Brock M. Miniard
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Influenza A H5N1 has emerged as a potential to be the next pandemic influenza. Ferrets are a promising model of H5N1 infection because the disease progression is similar to that known in humans, however reagents to characterize infections in ferrets are few. We developed real-time PCR assays for the ferret cytokines IL-1β, IL-2, IL-4, IL-8, IL-12p40, TNF-α, IFN-α, IFN-β, IFN-γ, TGFB-β1, and TGFB-β2. Forty-eight ferrets were vaccinated with either one of three vaccine formulations (Vaccine 1, Vaccine 2, or Vaccine 3), or saline (0.9% NaCl), then challenged intranasally with a lethal dose of H5N1 strain A/Vietnam/1203/04. Cytokine assays were then …
The Influence Of Stored Product Mites On The Function Of Skin Microvascular Endothelial Cells, Jennifer Graff Curp
The Influence Of Stored Product Mites On The Function Of Skin Microvascular Endothelial Cells, Jennifer Graff Curp
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The prevalence of allergenic diseases and sensitization to stored product mites is increasing worldwide. Stored product mites are prevalent in homes, foods, and some work environments. Stored product mites need to be further studied so we can characterize their inflammatory properties to understand their role in skin disease, and provide proper treatment to patients with mite allergies. The purpose of the study was to show the role of stored product mites in modulating the inflammatory and immune response to human dermal endothelial cells in the skin. The epidermis serves as the first line of defense between the body and the …
Immunomodulation Of Human Skin Cells By Extracts Of The Scabies Mite, Sarcoptes Scabiei, Jeremi Stevan Mullins
Immunomodulation Of Human Skin Cells By Extracts Of The Scabies Mite, Sarcoptes Scabiei, Jeremi Stevan Mullins
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Sarcoptes scabiei infests its host through burrowing into the stratum corneum layer of skin. Keratinocytes and fibroblasts responding to the burrowing mite(s) and/or mite products produce inflammatory and immune activating cytokines and chemokines. The purpose of this study was to determine if an extract of S. scabiei can modulate cytokine and chemokine expression from keratinocytes and fibroblasts in vitro. Normal human keratinocytes and fibroblasts were exposed to an extract of S. scabiei var. canis with or without various pro-inflammatory stimuli. Cytokine and chemokine expression were measured by enzyme-linked immunosorbent assay (ELISA). Scabies extract increased expression of interleukin-6 (IL-6), growth-related oncogene-α …