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Articles 1 - 18 of 18
Full-Text Articles in Life Sciences
Evaluating Forensic Dna Evidence - A Day-Long Workshop, Dan E. Krane, William C. Thompson
Evaluating Forensic Dna Evidence - A Day-Long Workshop, Dan E. Krane, William C. Thompson
Biological Sciences Faculty Publications
No abstract provided.
Keeping Insects At Bay: Beyond Deet, Thomas P. Rooney
Keeping Insects At Bay: Beyond Deet, Thomas P. Rooney
Biological Sciences Faculty Publications
No abstract provided.
Comparison Of C. Elegans And C. Briggsae Genome Sequences Reveals Extensive Conservation Of Chromosome Organization And Synteny, Ladeana W. Hiller, Raymond D. Miller, Scott Everet Baird, Asif Chinwalla, Lucinda A. Fulton, Daniel C. Koboldt, Robert H. Waterston
Comparison Of C. Elegans And C. Briggsae Genome Sequences Reveals Extensive Conservation Of Chromosome Organization And Synteny, Ladeana W. Hiller, Raymond D. Miller, Scott Everet Baird, Asif Chinwalla, Lucinda A. Fulton, Daniel C. Koboldt, Robert H. Waterston
Biological Sciences Faculty Publications
To determine whether the distinctive features of Caenorhabditis elegans chromosomal organization are shared with the C. briggsae genome, we constructed a single nucleotide polymorphism-based genetic map to order and orient the whole genome shotgun assembly along the six C. briggsae chromosomes. Although these species are of the same genus, their most recent common ancestor existed 80-110 million years ago, and thus they are more evolutionarily distant than, for example, human and mouse. We found that, like C. elegans chromosomes, C. briggsae chromosomes exhibit high levels of recombination on the arms along with higher repeat density, a higher fraction of intronic …
Essential Elements Of A Defense-Review Of Dna Testing Results, Dan E. Krane
Essential Elements Of A Defense-Review Of Dna Testing Results, Dan E. Krane
Biological Sciences Faculty Publications
No abstract provided.
Some Of The Problems Associated With Lcn (Low Copy Number) Testing, Dan E. Krane
Some Of The Problems Associated With Lcn (Low Copy Number) Testing, Dan E. Krane
Biological Sciences Faculty Publications
No abstract provided.
Run-Specific Limits Of Quantitation And Detection (An Alternative To Minimum Peak Height Thresholds), Dan E. Krane
Run-Specific Limits Of Quantitation And Detection (An Alternative To Minimum Peak Height Thresholds), Dan E. Krane
Biological Sciences Faculty Publications
No abstract provided.
Empirical Analysis Of The Str Profiles Resulting From Conceptual Mixtures, Dan E. Krane
Empirical Analysis Of The Str Profiles Resulting From Conceptual Mixtures, Dan E. Krane
Biological Sciences Faculty Publications
No abstract provided.
Assessing The Implications For Close Relatives In The Event Of Similar But Non-Matching Dna Profiles, Dan E. Krane
Assessing The Implications For Close Relatives In The Event Of Similar But Non-Matching Dna Profiles, Dan E. Krane
Biological Sciences Faculty Publications
No abstract provided.
Preliminary Notes On Tachinidae Reared From Lepidoptera In The Ecuadorian Andes, John O. Stireman Iii
Preliminary Notes On Tachinidae Reared From Lepidoptera In The Ecuadorian Andes, John O. Stireman Iii
Biological Sciences Faculty Publications
Current estimates of the species richness of Tachinidae among geographical provinces suggest that the Neotropical Region harbors the largest number of species and represents a geographic epicenter of tachinid diversification (O’Hara 2006). The Neotropics boasts an impressive fauna consisting of 2864 described species belonging to 822 genera at the time of the Neotropical catalog (Guimarães 1971), making it almost twice as species rich as any other geographic realm (O’Hara 2006; Stireman et al. 2006). This diversity is most apparent at middle elevations (1000– 2000m) along the mountain chains of tropical Central and South America, where tachinids are an abundant and …
Distribution Of Ground-Layer Plant Species In A Fragmented Landscape In The Corozal District, Belize, Central America, Divan Catzim
Distribution Of Ground-Layer Plant Species In A Fragmented Landscape In The Corozal District, Belize, Central America, Divan Catzim
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In this study, I specifically looked at three major issues regarding 36 target plant species in a fragmented landscape in the Corozal district, Belize: 1) Determine woodlot characteristics that tend to favor the target species. 2) Determine plant characteristics that tend to favor the target species. 3) Determine whether age and size of a woodlot favors the presence of the target species. The study area was divided into three areas and each area had woodlots varying in sizes and ages. 36 plant species were sampled in all woodlots upon which woodlot characteristics and species characteristics were associated with the number …
Autoregulation Of Gld-2 Cytoplasmic Poly(A) Polymerase, Labib Rouhana, Marvin Wickens
Autoregulation Of Gld-2 Cytoplasmic Poly(A) Polymerase, Labib Rouhana, Marvin Wickens
Biological Sciences Faculty Publications
Cytoplasmic polyadenylation regulates mRNA stability and translation and is required for early development and synaptic plasticity. The GLD-2 poly(A) polymerase catalyzes cytoplasmic polyadenylation in the germline of metazoa. Among vertebrates, the enzyme is encoded by two isoforms of mRNA that differ only in the length of their 3′-UTRs. Here we focus on regulation of vertebrateGLD-2 mRNA. We show that the 3′-UTR of GLD-2 mRNA elicits its own polyadenylation and translational activation during frog oocyte maturation. We identify the sequence elements responsible for repression and activation, and demonstrate that CPEB and PUF proteins likely mediate repression in the resting oocyte. …
Tree Seedling Establishment Under The Native Shrub, Asimina Triloba, Marilyn Cabrini Baumer
Tree Seedling Establishment Under The Native Shrub, Asimina Triloba, Marilyn Cabrini Baumer
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Species that form forest understory layers affect canopy tree seedling establishment worldwide. In the Eastern United States, shrub understories like Rhododendrom maximum, a native evergreen species, and Lonicera maackii, an exotic, invasive species, diminish tree seedling survival. I compared the density and survival of canopy tree seedlings under and outside patches of the native shrub, Asimina triloba (L.) Dunal (Annonaceae) (pawpaw). I also conducted a manipulative experiment to determine whether above ground or below ground competition was more important in seedling growth and survival. Above ground competition was manipulated by tying back the leaves of the pawpaw and below ground …
Modulation Of The 3'Igh Regulatory Region (3'Igh Rr), A Prospective In Vitro Screening Tool For Identifying Potential Immunotoxicants, Rebecca Anne Henseler
Modulation Of The 3'Igh Regulatory Region (3'Igh Rr), A Prospective In Vitro Screening Tool For Identifying Potential Immunotoxicants, Rebecca Anne Henseler
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The immune system is critical to human survival. However, assessing alterations of immune function by potential immunotoxicants is complicated by the diffuse nature of the immune system, which is composed of various effector cells each with differing effector functions. Current immunotoxicity testing is limited to animal studies. We have developed a model, which may provide an in vitro alternative to animal studies in identifying immunotoxicants that specifically target B cell function (i.e., alteration of immunoglobulin (Ig) or expression and antibody secretion). This model consists of a well-characterized B cell line, CH12.LX, which appears to appropriately model primary B cell function. …
Molecular Identification Of Chironomid Species Based On Its-1 And Its-2 Regions Of Rdna, Monita Sharma
Molecular Identification Of Chironomid Species Based On Its-1 And Its-2 Regions Of Rdna, Monita Sharma
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Of all major aquatic invertebrate groups, members of family Chironomidae are most abundant and show a wide range of habitat preferences. The importance of correct identification of Chironomids has been realized in many bioassessment studies mainly because of their worldwide distribution, substrate specificities and predictable responses to various pollutants in the water sources. This study establishes that the sequence data from the Intergenic Spacer Regions (ITS) of ribosomal DNA could be used as molecular markers to distinguish between different Chironomidae species and also to identify them. The need to use molecular approaches, to identify various Chironomidae species, comes from the …
Acute Toxicity And Immunotoxicity Testing Of Total Petroleum Hydrocarbons In Aquatic And Terrestrial Organisms, Andrea Renee Handy
Acute Toxicity And Immunotoxicity Testing Of Total Petroleum Hydrocarbons In Aquatic And Terrestrial Organisms, Andrea Renee Handy
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The acute toxicity and immunotoxicity of JP-8 jet fuel on Chironomus tentans, Hyallela azteca, Lactuca sativa, Eisenia foetida, and Lumbricus terrestris was assessed using standard USEPA acute toxicity and static renewal toxicity tests. Three methods of spiking test soil with jet fuel were evaluated. In one method acetone was utilized as a carrier and the soil was dried in the fume hood; in another the soil was spiked directly with jet fuel and also was dried; and in the last the soil was spiked directly without drying. There was low survival in C. tentans in all treatments, including controls. There …
Investigation Of Interactions Between Rev And Microtubules: Purification Of Wild-Type And Mutant Rev Protein And Optimization Of Microtubule Depolymerization Assays, Miller, Kelly Robbins
Investigation Of Interactions Between Rev And Microtubules: Purification Of Wild-Type And Mutant Rev Protein And Optimization Of Microtubule Depolymerization Assays, Miller, Kelly Robbins
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As a logical pharmaceutical target for antiviral drugs, HIV-1 Rev is a regulatory protein essential for viral infection (Hope, 1999). The development of antiviral drugs that target Rev has been hindered by the lack of high-resolution structural information due to the protein's tendency to aggregate in solution. While searching for solution conditions rendering Rev amenable to crystallographic analyses, Watts et al., (2000) discovered a novel in vitro interaction between Rev and microtubules (MTs) whereby addition of equimolar Rev and tubulin forms bilayered rings called Rev-tubulin toroidal complexes (RTTs). RTTs are similar to those seen when MTs are mixed with certain …
Analysis Of Sex Reversal And Tra-2 Nucleotide Variation In Tropical And Temperate Clades Of Caenorhabditis Briggsae, Irene Zelepuhin
Analysis Of Sex Reversal And Tra-2 Nucleotide Variation In Tropical And Temperate Clades Of Caenorhabditis Briggsae, Irene Zelepuhin
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Reproductive isolation results when members of the same species cannot reproduce due to either prezygotic or postzygotic mechanisms, therefore restricting gene flow between populations. A leading model describing reproductive isolation developed by J.B. Haldane (1922) states, "When offspring from two different animal species have one sex that is rare, absent, or sterile, that sex is the heterozygous (XO) or heterogametic (XY) sex". Haldane's rule is illustrated among various animal taxa regardless of which sex is heterogametic. To date, Caenorhabditis mating tests are the only example of Haldane's rule that is caused by sexual transformation and not gender specific lethality or …
Immunological And Developmental Effects Of Polybrominated Diphenyl Ethers (Pbdes) And 2,3,7,8-Tetrachloro-P-Dioxin (Tcdd) In Birds, Randy T. Stetzer
Immunological And Developmental Effects Of Polybrominated Diphenyl Ethers (Pbdes) And 2,3,7,8-Tetrachloro-P-Dioxin (Tcdd) In Birds, Randy T. Stetzer
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This thesis contains two separate and distinct research projects with a common overall theme - the study of immunotoxicity associated with the developmental exposure of young birds to halogenated chemicals posing environmental concern. These chemicals share many chemical characteristics, including environmental ubiquity and longevity, one has been studied extensively since the 1970s, and one is a relatively new environmental contaminant with few studies pertaining to toxicological affects on humans or wildlife. Specific summaries of each study begin each project chapter. The first study was entitled Developmental and Immunological Effects of a Commercial Mixture of PBDE Flame Retardants in Chicken Embryos. …