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Budding Yeast Protein Extraction And Purification For The Study Of Function, Interactions, And Post-Translational Modifications, Eva P. Szymanski, Oliver Kerscher
Budding Yeast Protein Extraction And Purification For The Study Of Function, Interactions, And Post-Translational Modifications, Eva P. Szymanski, Oliver Kerscher
Arts & Sciences Articles
Homogenization by bead beating is a fast and efficient way to release DNA, RNA, proteins, and metabolites from budding yeast cells, which are notoriously hard to disrupt. Here we describe the use of a bead mill homogenizer for the extraction of proteins into buffers optimized to maintain the functions, interactions and post-translational modifications of proteins. Logarithmically growing cells expressing the protein of interest are grown in a liquid …