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Sympatric Ecological Speciation Meets Pyrosequencing: Sampling The Transcriptome Of The Apple Maggot Rhagoletis Pomonella, Dietmar Schwarz, Hugh M. Robertson, Jeffrey L. Feder, Kranthi Varala, Matthew E. Hudson, Gregory J. Ragland, Daniel A. Hahn, Stewart H. Berlocher
Sympatric Ecological Speciation Meets Pyrosequencing: Sampling The Transcriptome Of The Apple Maggot Rhagoletis Pomonella, Dietmar Schwarz, Hugh M. Robertson, Jeffrey L. Feder, Kranthi Varala, Matthew E. Hudson, Gregory J. Ragland, Daniel A. Hahn, Stewart H. Berlocher
Biology Faculty and Staff Publications
Background
The full power of modern genetics has been applied to the study of speciation in only a small handful of genetic model species - all of which speciated allopatrically. Here we report the first large expressed sequence tag (EST) study of a candidate for ecological sympatric speciation, the apple maggot Rhagoletis pomonella, using massively parallel pyrosequencing on the Roche 454-FLX platform. To maximize transcript diversity we created and sequenced separate libraries from larvae, pupae, adult heads, and headless adult bodies.
Results
We obtained 239,531 sequences which assembled into 24,373 contigs. A total of 6810 unique protein coding genes …
A Comparative Study Of Drosophila And Human A-Type Lamins, Sandra R. Schulze, Beatrice Curio-Penny, Sean Speese, George Dialynas, Diane E. Cryderman, Cairtrin W. Mcdonough, Demet Nalbant, Melissa Petersen, Vivian Budnik, Pamela K. Geyer, Lori L. Wallrath
A Comparative Study Of Drosophila And Human A-Type Lamins, Sandra R. Schulze, Beatrice Curio-Penny, Sean Speese, George Dialynas, Diane E. Cryderman, Cairtrin W. Mcdonough, Demet Nalbant, Melissa Petersen, Vivian Budnik, Pamela K. Geyer, Lori L. Wallrath
Biology Faculty and Staff Publications
Nuclear intermediate filament proteins, called lamins, form a meshwork that lines the inner surface of the nuclear envelope. Lamins contain three domains: an N-terminal head, a central rod and a C-terminal tail domain possessing an Ig-fold structural motif. Lamins are classified as either A- or B-type based on structure and expression pattern. The Drosophila genome possesses two genes encoding lamins, Lamin C and lamin Dm0, which have been designated A- and B-type, respectively, based on their expression profile and structural features. In humans, mutations in the gene encoding A-type lamins are associated with a spectrum of predominantly tissue-specific diseases …