Life Sciences Commons^™

Characterization Of Etoxazole Resistance In Tetranychus Urticae Populations Collected From Commercial Greenhouses & Fields In Ontario, Jeremy Spenler

Electronic Thesis and Dissertation Repository

In this investigation, etoxazole resistance was monitored in two spotted spider mite (TSSM) populations collected from commercial greenhouses in Ontario. The frequency of an etoxazole resistant, recessive target-site mutation, I1017F, in chitin synthase I (CHS1) was tracked within populations over the course of the study. Cytochrome P450 (P450) activity emerged as a possible alternative mechanism to resist etoxazole exposure in one population.

Using genetic crossing between highly resistant mite populations and a susceptible lab population, filial (F1) hybrid populations were created. The recessive I1017F target-site mutation was abolished in F1 hybrids highly susceptible to etoxazole, while elevated P450 …

Development Of Reverse Genetics Tools In The Two-Spotted Spider Mite Tetranychus Urticae, Nivitha Bhaskar

Electronic Thesis and Dissertation Repository

Tetranychus urticae, commonly known as the two-spotted spider mite, poses a significant threat to agriculture due to its ability to feed on a diverse range of plant hosts and its strong detoxification abilities in overcoming xenobiotic response. With global warming projected to increase spider mite infestations, it is vital to study the detoxification genes that enable the mite to adapt and survive. The spider mite genome sequence reveals a unique set of detoxification genes that can be studied using RNAi as a promising reverse genetic tool. However, the current genetic toolkit requires improvement. This study examined the effectiveness of …