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Full-Text Articles in Life Sciences

Two Spotted Spider Mite (Tetranychus Urticae) Selection To Arabidopsis Thaliana, Huzefa Ratlamwala Aug 2014

Two Spotted Spider Mite (Tetranychus Urticae) Selection To Arabidopsis Thaliana, Huzefa Ratlamwala

Electronic Thesis and Dissertation Repository

Spider mite feeding on A. thaliana induces the production of indole glucosinolates (IGs), plant secondary metabolites that negatively affect mite performance. In this study I conducted selection experiments on A. thaliana with varying levels of IGs, to determine if mites could adapt to IGs and other defense compounds. After 12 months, mites reared on host with IGs performed significantly better on A. thaliana than mites maintained on beans. However, an adaptation cost was detected between selected mite lines and their ancestral host. The qRT-PCR data on different mite lines revealed that the detoxification genes previously identified may only be involved …


Arabidopsis Thaliana-Spider Mite Interaction: Plant Perception, Signalling, And Response, Kristie A. Bruinsma Apr 2014

Arabidopsis Thaliana-Spider Mite Interaction: Plant Perception, Signalling, And Response, Kristie A. Bruinsma

Electronic Thesis and Dissertation Repository

The two-spotted spider mite, Tetranychus urticae, is a cell-content feeding chelicerate herbivore, feeding on over 1000 plant species, one of which is Arabidopsis thaliana. This research uses microarray data from two A. thaliana accessions that differ in susceptibility to spider mite feeding to identify how the plant defends itself against this herbivore. Mutant analysis of induced plant defense pathways and physiological assays of mite performance indicate that A. thaliana utilizes: a) damage associated molecular pattern receptors, PEPR1 and PEPR2, to aid in perception of attack; b) jasmonic acid as the key phytohormone involved in resistance signalling; and c) …