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Characterization Of Squamosa Promoter Binding Protein Like (Spl), And Its Role In Drought Stress Tolerance In Medicago Sativa (Alfalfa)., Alexandria K. Hanly
Characterization Of Squamosa Promoter Binding Protein Like (Spl), And Its Role In Drought Stress Tolerance In Medicago Sativa (Alfalfa)., Alexandria K. Hanly
Electronic Thesis and Dissertation Repository
Traditional breeding involving Medicago sativa (alfalfa), has resulted in minimal yield increases. Moreover, extreme environmental conditions threaten to further limit production. Strategies that make use of molecular tools – such as small non-coding RNA, miR156 – represent an innovative means by which to influence tolerance to abiotic stress. miR156 functions, at least in part, through the SQUAMOSA PROMOTER BINDING PROTEIN LIKE (SPL) family of transcription factors. In this study, the role of SPL9 in regulating alfalfa development and drought tolerance is evaluated. Examination of alfalfa plants with RNAi-mediated SPL9 (SPL9-RNAi) showed that plant height, stem thickness, and internode …
Microrna156: A Short Rna With A Major Role In Abiotic Stress Tolerance In Alfalfa, Biruk Ayenew Feyissa
Microrna156: A Short Rna With A Major Role In Abiotic Stress Tolerance In Alfalfa, Biruk Ayenew Feyissa
Electronic Thesis and Dissertation Repository
The highly conserved plant microRNA156, miR156, affects various aspects of plant development and stress response by silencing SQUAMOSA-PROMOTER BINDING PROTEIN-LIKE (SPL) transcription factors. Our understanding of the role of miR156 and its mode of action in alfalfa’s (Medicago sativa L.) response to drought and flooding is still elusive, and thus this study was aimed at filling this gap in knowledge. Physiological parameters, metabolite and transcriptional analyses showed an interplay between miR156/SPL13 and WD40-1/DFR to mitigate drought stress. Low to moderate levels of miR156 overexpression suppressed SPL13 and increased WD40-1 to fine-tune the DIHYDROFLAVONOL-4-REDUCTASE (DFR) level for …
Quantitative Proteome Analysis Of Alfalfa In Drought Stress Under The Influence Of Mir156, Alpa Puri
Quantitative Proteome Analysis Of Alfalfa In Drought Stress Under The Influence Of Mir156, Alpa Puri
Electronic Thesis and Dissertation Repository
Medicago sativa is one of the most produced perennial forage crops in Canada. Drought stress is a major form of abiotic stress affecting the productivity and annual yield of alfalfa. A small noncoding RNA, miR156 has a role in abiotic stress tolerance, while regulating downstream SPL genes. In this study, whole leaf proteome and amino acid concentrations of miR156OE (A8) genotype of alfalfa under drought stress were quantified. In results, 3,000 proteins groups were identified using MaxQuant when searched against Medicago truncatula protein sequence database. Gene ontology analysis showed enriched proteins were involved in biological and molecular processes such as …
Characterizing The Role Of The Mir156-Spl Network In Heat Stress Response In Medicago Sativa, Craig Matthews
Characterizing The Role Of The Mir156-Spl Network In Heat Stress Response In Medicago Sativa, Craig Matthews
Electronic Thesis and Dissertation Repository
Heat stress negatively impacts plant development by disrupting regular plant functions, including molecular, physiological and anatomical processes, reducing crop production. Alfalfa (Medicago sativa) is an important forage crop and developing abiotic stress resistant cultivars would help mitigate crop losses. Members of the miR156 family regulate SPL genes, impacting plant growth and development and are involved in stress response. Here, alfalfa with miR156 overexpression (miR156OE) and SPL13 RNAi knockdown (SPL13i) showed increased tolerance to heat stress (40°C) while SPL9 RNAi alfalfa did not. Heat-stressed miR156OE and SPL13i plants had increased antioxidant levels, including anthocyanins. Additionally, …