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Articles 1 - 2 of 2
Full-Text Articles in Life Sciences
Individual Interactions Of The B Subunits Within The Stator Of The Escherichia Coli Atp Synthase., Karsten Brandt, Sarah Maiwald, Brigitte Herkenhoff-Hesselmann, Kerstin Gnirss, Joerg-Christian Greie, Stanley D Dunn, Gabriele Deckers-Hebestreit
Individual Interactions Of The B Subunits Within The Stator Of The Escherichia Coli Atp Synthase., Karsten Brandt, Sarah Maiwald, Brigitte Herkenhoff-Hesselmann, Kerstin Gnirss, Joerg-Christian Greie, Stanley D Dunn, Gabriele Deckers-Hebestreit
Biochemistry Publications
FOF1 ATP synthases are rotary nanomotors that couple proton translocation across biological membranes to the synthesis/hydrolysis of ATP. During catalysis, the peripheral stalk, composed of two b subunits and subunit δ in Escherichia coli, counteracts the torque generated by the rotation of the central stalk. Here we characterize individual interactions of the b subunits within the stator by use of monoclonal antibodies and nearest neighbor analyses via intersubunit disulfide bond formation. Antibody binding studies revealed that the C-terminal region of one of the two b subunits is principally involved in binding of subunit δ, while the other one is accessible …
Fuzzy Complex Formation Between The Intrinsically Disordered Prothymosin Α And The Kelch Domain Of Keap1 Involved In The Oxidative Stress Response., Halema Khan, Elio A Cino, Anne Brickenden, Jingsong Fan, Daiwen Yang, Wing-Yiu Choy
Fuzzy Complex Formation Between The Intrinsically Disordered Prothymosin Α And The Kelch Domain Of Keap1 Involved In The Oxidative Stress Response., Halema Khan, Elio A Cino, Anne Brickenden, Jingsong Fan, Daiwen Yang, Wing-Yiu Choy
Biochemistry Publications
Kelch-like ECH-associated protein 1 (Keap1) is an inhibitor of nuclear factor erythroid 2-related factor 2 (Nrf2), a key transcription factor for cytoprotective gene activation in the oxidative stress response. Under unstressed conditions, Keap1 interacts with Nrf2 in the cytoplasm via its Kelch domain and suppresses the transcriptional activity of Nrf2. During oxidative stress, Nrf2 is released from Keap1 and is translocated into the nucleus, where it interacts with the small Maf protein to initiate gene transcription. Prothymosin α (ProTα), an intrinsically disordered protein, also interacts with the Kelch domain of Keap1 and mediates the import of Keap1 into the nucleus …