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Generation Of Transgenic Medicago Sativa Overexpressing "Osmotin-Chitinase" Gene Chimera, Jahnavi Reddy Kancharla
Generation Of Transgenic Medicago Sativa Overexpressing "Osmotin-Chitinase" Gene Chimera, Jahnavi Reddy Kancharla
Masters Theses & Specialist Projects
Medicago is widely used as a forage crop. It is often susceptible to various pathogenic infections and exhibits low growth in drought and extreme climatic conditions. In the current study, a strategy was developed for over-expressing an “Osmotin-Chitinase” gene chimera in transgenic Medicago that could potentially confer resistance to different biotic and abiotic stresses. Seed germination of several cultivars of Medicago (M. sativa ssp. sativa, M. sativa ssp. falcata, M. sativa ssp. caerulea, M. truncatula, and M. Rugosa) was tested to determine the cultivars with good germination rates. Among these, M. sativa ssp. sativa showed an …