Life Sciences Commons^™

Sound Production In Two Loricariid Catfishes, Amanda Lynn Webb

Masters Theses & Specialist Projects

Many families of catfish produce sounds via pectoral spine stridulation and/or swim bladder compression using sonic muscles attached to the swim bladder. The sound production capabilities and characteristics in Loricariidae, the largest catfish family, have not been well examined. Sounds produced by two loricariid catfish species, Macrotocinclus affinis and Pterygoplichthys gibbiceps were recorded. Both species produce broad band calls via pectoral spine stridulation. These species produce sounds by rubbing the ridges of the dorsal process of the pectoral spine base against the groove of the pectoral girdle. Call duration was generally shorter in M. affinis (2-15 ms) as opposed to …

Action Spectrum For Photoentrainment Of The Circadian Clock In Wild-Type Chlamydomonas Reinhardtii, Jennifer Forbes=Stovall

Masters Theses & Specialist Projects

The circadian clock is an endogenous timer that oscillates with a period of approximately 24 hours and is reset upon environmental time cues such as the daily light/ dark or temperature cycles. Chlamydomonas reinhardtii is an ideal model organism for research on the circadian clock, because it shows several well-characterized behaviors that exhibit a circadian rhythm. Its circadian rhythm of phototaxis (swimming toward light) has been automated. Former action spectrum studies using the circadian phototaxis rhythm as an indicator surprisingly found that pulses of blue light were not effective in resetting the circadian clock of dark-adapted cells. This may have …

Pcr Amplification Of Trypanosoma Cruzi - Specific Dna From Raccoon Blood Samples, Colin Moss

Mahurin Honors College Capstone Experience/Thesis Projects

The parasitic protist Trypanosoma cruzi is the causative agent of Chagas Disease. Chagas Disease causes greater than 15,000 deaths each year, and nearly 28 million people are believed to be at risk of infection in Central and South America. This parasite has been described in many mammalian host species and has also been described in the United States. The purpose of this study was to attempt to use PCR to amplify T. cruzi-specific DNA directly from blood samples obtained from raccoons (Procyon lotor) trapped in Warren and Barren Counties of Kentucky in 2007 and 2008. DNA was …

Assessing Social Benefits Of Domestic Rainwater Harvesting In Southern Kenya, Lindsey M. Filiatreau

Mahurin Honors College Capstone Experience/Thesis Projects

In the Kasigau location of the Voi District of Kenya, severe rainwater shortages closed fourteen water collection stations in 2008 and 2009, leaving ten water sources to service 15,000 people in the region. Few families in the area have domestic rainwater harvesting systems which are an easily implemented, low-cost means of water collection. I investigated the ability of rainwater catchment systems to modify family time budgets (by reducing water collection time) and lessen the severity of water scarcity (by supplying families a significant amount of water). Forty families participated in the research, 20 of whom received free installation of a …

Once On The Dark Continent, Katrina Alexandria Bidwell

Mahurin Honors College Capstone Experience/Thesis Projects

Set in the fictional village of Buguta in post-genocide Rwanda, Once on the Dark Continent follows the experiences of a young doctor working for the Doctors Without Borders Program. Dr. Erica Davis unwittingly finds herself in a country where the past has continued to repeat itself a decade after a devastating civil war, unbeknownst to most of the outside world. Their history isn’t the only thing plaguing the villagers like a parasite: Erica’s careful attention to the patients at the Buguta clinic leads her to diagnose them with toxoplasmosis, a revelation that has the potential to begin solving the myriad …

The Proliferation Of Vascular Smooth Muscle Cells Depends On Thioredoxin 1 Protein In A Model Of Pulmonary Hypertension, Viktoria Nelin

Mahurin Honors College Capstone Experience/Thesis Projects

Pulmonary hypertension (PH) is a disease which manifests itself in the lungs of both adults and children. Vascular proliferation and remodeling are the hallmarks of PH and are found mainly in the pulmonary arterial smooth muscle cells (PASMC). The cause of PASMC proliferation and vascular remodeling in PH is poorly understood. Hypoxia, or low oxygen content, underlies many forms of PH. Hypoxia results in alterations in the redox balance of the PASMC. Therefore, the aim of this study was to examine the role of the thioredoxin system, an antioxidant system in the cell, in hypoxia-induced proliferation in PASMC. Protein was …

Screening Rnai Transformants Of Chlamydomonas For Reduced Expression Of The Photoreceptor Cryptochrome, Jeremy Webb

Mahurin Honors College Capstone Experience/Thesis Projects

Chlamydomonas reinhardtii is a unicellular green alga. In C. reinhardtii, three types of photoreceptors are known to be present: rhodopsins, phototropins, and cryptochromes. The single cryptochrome is the most likely photoreceptor for adjusting the circadian clock to the daily light/dark cycles, because cryptochromes are involved in clock entrainment in higher plants and insects. In this segment of the research, C. reinhardtii strains, which were genetically modified through transformation with a RNA interference construct, were screened for reduction in cryptochrome compared to the control strain. After C. reinhardtii cultures were harvested in complete darkness, all soluble proteins were extracted from the …

Dna Sequence Variation In The Wingless Gene Product In Buckeye Butterflies (Genus Junonia), Bonnie Mccullagh

Mahurin Honors College Capstone Experience/Thesis Projects

Wingless is a highly conserved gene important to cell determination in development. In Drosophila, the wingless gene product has been identified as responsible for wing patterning. In Bicyclus anynana and Junonia coenia, wingless gene product is expressed in a fashion that suggests that it is involved in butterfly wing color pattern development. The wingless gene product has been implicated as a potential focal signal for patterning the eyespot of Junonia butterflies. I have shown that extensive DNA sequence variation (26.04% of the sequenced region) exists in 402 bp of wingless coding sequence among 338 specimens of Junonia from …

Avian Abundance And Diversity In Crep And Fescue Fields In The Summer And Winter Related To Vegetation Structure In The Upper Green River Watershed, Ky, Thomas A. Hulsey

Mahurin Honors College Capstone Experience/Thesis Projects

Grassland bird populations have been undergoing dramatic declines in the last fifty years due to loss and degradation of habitat. The Conservation Reserve Enhancement Program (CREP) was initiated in the upper Green River Watershed of south-central Kentucky to improve water quality and provide wildlife habitat. Many studies have previously examined how similar programs in other states have contributed to grassland bird populations; in this study we are examining how the Kentucky CREP program is influencing bird populations, particularly grassland birds, and contributing to the conservation of these species. Bird and vegetation surveys were conducted during the summer of 2008 and …

Assessment Of Prenatal Care And Perinatal Outcomes In Kasigau, Kenya, Allison Smith

Mahurin Honors College Capstone Experience/Thesis Projects

The World Health Organization (WHO) implemented the Millennium Declaration in 2000 to establish global health goals to be achieved by 2015. Two of these Millennium Development Goals (MDG’s) are directly related to maternal, neonatal and child health. Healthcare during the prenatal period can help achieve the WHO MDG’s for maternal health and child mortality. Evidence has shown that women in Kenya have limited access to prenatal care. This study focused on the prenatal care and postnatal outcome trends of the women in Kasigau, Kenya. Participants were surveyed in three villages (Rukanga, Makwasinyi, and Bhuguta) to determine if prenatal care was …

Human-Wildlife Conflict On Small, Subsistence Farms In Kenya, Christopher B. Colonna

Mahurin Honors College Capstone Experience/Thesis Projects

As human populations expand, wildlife suddenly competes with humans for resources and confrontation arises as a result. Rural Africa is typical of this problem. We surveyed local owners of small farms within the five villages surrounding Mount Kasigau in Southeast Kenya to quantify losses due to wildlife depredation on both subsistence and cash crops as well as to discover the patterns and variables influencing farmer-wildlife confrontations in the region. We found no statistically significant correlations among the value of damage per acre, the distance from the bush, or the distance to the nearest water source. We did find statistical significance …

Prescribed Fire Effects On The Summer And Fall Herbs Of Mesic Deciduous Forests, Margaret Wilder

Mahurin Honors College Capstone Experience/Thesis Projects

After years of fire suppression, high intensity forest fires were destructive to surrounding areas. Historically, fire was common in the eastern United States, but was suppressed over the past century, and recently has become a major tool in forest management. But to date, there have been no studies on the influence of fire on mesic sites in the eastern United States. Because fire is being reintroduced as a management practice, it is critical to know the influence of fire in this region. This study seeks to understand the influences of fire on summer and fall herbs in the western mesophytic …

The History Of Zoological Gardens And The State, Federal And International Laws That Govern Them, Morgan Maxwell

Mahurin Honors College Capstone Experience/Thesis Projects

The history of zoos in North America can be traced back through 18th century Europe to ancient civilizations such as the Romans. The menageries of ancient Rome were far different from modern zoos that stress conservation, education, and research as primary objectives. Zoos are still evolving and changing to become more suitable for captive animals and to ensure their animals’ health and well-being. An examination of state wildlife laws reveals that there are more laws that pertain to wildlife at the state and local level than at any other level of government. Federal wildlife laws are few in number and …

Effective And Affordable Novel Arsenic Removal Technology, Benadin Varajic

Mahurin Honors College Capstone Experience/Thesis Projects

Arsenic contaminated drinking water is a serious worldwide issue faced by millions of people every day. Long term exposure to arsenic levels above the World Health Organization’s (WHO) standard of 10 ppb has been associated with severe detrimental health effects including cardiovascular disease and cancer. Although current water purification technologies exist, their implementation is an unrealistic goal by many third world nations for a variety of reasons, the most common being the cost associated with what are typically complicated procedures. This study strives to discover an effective and affordable novel arsenic removal technology.

The first step of this study was …

Generation Of Transgenic Medicago Sativa Overexpressing "Osmotin-Chitinase" Gene Chimera, Jahnavi Reddy Kancharla

Masters Theses & Specialist Projects

Medicago is widely used as a forage crop. It is often susceptible to various pathogenic infections and exhibits low growth in drought and extreme climatic conditions. In the current study, a strategy was developed for over-expressing an “Osmotin-Chitinase” gene chimera in transgenic Medicago that could potentially confer resistance to different biotic and abiotic stresses. Seed germination of several cultivars of Medicago (M. sativa ssp. sativa, M. sativa ssp. falcata, M. sativa ssp. caerulea, M. truncatula, and M. Rugosa) was tested to determine the cultivars with good germination rates. Among these, M. sativa ssp. sativa showed an …

The Transition From 3-D To 2-D Visual Stimuli And Its Effect On Paired-Associate Learning, Amanda Beers

Mahurin Honors College Capstone Experience/Thesis Projects

A single experiment investigated the influence of varying dimensions of visual stimuli on the ability of academically gifted adolescents to recall paired-associations. Fifty-two participants were randomly assigned to one of four groups. Each group was asked to memorize the same set of 15 paired-word associations. The first group was presented with words only. The second group was presented with words and 2- dimensional (2-D) representations of each word. The third group was presented with words along with 3-dimensional (3-D) representations of the words. The fourth group was also presented words along with 3-D objects; however, this group was instructed to …