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Full-Text Articles in Life Sciences

Effects Of Starch Concentration On Calcium‐Enhanced Black Bullhead Catfish Protein Gels, Ilgin Paker, Kristen E. Matak Jan 2017

Effects Of Starch Concentration On Calcium‐Enhanced Black Bullhead Catfish Protein Gels, Ilgin Paker, Kristen E. Matak

Faculty & Staff Scholarship

Calcium‐enhanced protein recovered from black bullhead catfish was used to develop gels containing increasing amounts of potato starch (0–20 g/kg protein paste) and the effects of starch on functional, textural, and color properties were tested. Energy required to unfold protein groups was greater with the addition of 5 g starch/kg protein paste. Gels containing starch were harder, chewier, and less springy (p < .05) than gels without starch. For most measurements, regression analysis showed that increasing the starch concentration beyond 5 g/kg did not contribute to further significant textural changes. Torsional shear stress and strain along with Kramer shear force increased as the concentration of starch increased (R2 = .79, .79, and .53, respectively). The addition of ≥10 g starch/kg protein paste resulted in darker gels and gels got darker as more starch was added (R2 = .71). Results showed no …


Re-Framing Biotechnology Regulation, Alison Peck Jan 2017

Re-Framing Biotechnology Regulation, Alison Peck

Law Faculty Scholarship

Biotechnology is about to spill the banks of federal regulation. New genetic engineering techniques like CRISPR-Cas9 promise revolutionary breakthroughs in medicine, agriculture, and public health-but those techniques would not be regulated under the terms of the Coordinated Framework for Regulation of Biotechnology. This revolutionary moment in biotechnology offers an opportunity to correct the flaws in the framework, which was hastily patched together at the advent of the technology. The framework has never captured all relevant technologies, has never satisfied the public that risk is being effectively managed, and has never been accessible to small companies and publicly-funded labs that increasingly …


Efficacy Of Nutritional Interventions To Lower Circulating Ceramides In Young Adults: Fruvedomic Pilot Study, Alice T. Matthews, Oluremi A. Famodu, Melissa D. Olfert, Pamela J. Murray, Christopher F. Cuff, Marianne T. Downes, Norman J. Haughey, Sarah E. Colby, Paul D. Chantler, I. Mark Olfert, Joseph W. Mcfadden Jan 2017

Efficacy Of Nutritional Interventions To Lower Circulating Ceramides In Young Adults: Fruvedomic Pilot Study, Alice T. Matthews, Oluremi A. Famodu, Melissa D. Olfert, Pamela J. Murray, Christopher F. Cuff, Marianne T. Downes, Norman J. Haughey, Sarah E. Colby, Paul D. Chantler, I. Mark Olfert, Joseph W. Mcfadden

Faculty & Staff Scholarship

The 2010 USDA Dietary Guidelines for Americans (DGA) recommends a diet largely composed of fruit and vegetables. Consuming a diet high in fruit and vegetables and low in refined carbohydrates and saturated fat may reduce an individual’s risk for type 2 diabetes, nonalcoholic fatty liver disease, low-grade chronic inflammation, and metabolic syndrome (MetS). Several recent studies have implicated the bioactive sphingolipid ceramide as an associative and causative biomarker for the development of these conditions. Considering that the intake of fruit and vegetables is frequently inadequate in young adults, we performed a pilot investigation to assess the efficacy of a free-living …


Wetland Seed Dispersal By White-Tailed Deer In A Large Freshwater Wetland Complex, Kelley L. Flaherty, James S. Rentch, James T. Anderson Jan 2017

Wetland Seed Dispersal By White-Tailed Deer In A Large Freshwater Wetland Complex, Kelley L. Flaherty, James S. Rentch, James T. Anderson

Faculty & Staff Scholarship

Mechanisms of long-distance dispersal are important in establishing and maintaining plant populations in isolated wetland habitats. White-tailed deer (Odocoileus virginianus) have been cited as long-distance dispersers of both native and exotic plant species in North America; however, knowledge regarding their influence in wetlands is limited. Given traditional classification methods for seed dispersal, white-tailed deer are not likely viewed as important dispersal mechanism for wetland plants. We collected naturally deposited white-tailed deer faecal pellet piles from wetlands in Canaan Valley, West Virginia, USA. Pellet piles were cold-stratified and germinated seedlings over a layer of sterile potting mix. The percentage of germinated …


Factors Affecting Availability For Detection: An Example Using Radio-Collared Northern Bobwhite (Colinus Virginianus), Christopher M. Lituma, David A. Buehler, Evan P. Tanner, Ashley M. Tanner, Patrick D. Keyser, Craig A. Harper Jan 2017

Factors Affecting Availability For Detection: An Example Using Radio-Collared Northern Bobwhite (Colinus Virginianus), Christopher M. Lituma, David A. Buehler, Evan P. Tanner, Ashley M. Tanner, Patrick D. Keyser, Craig A. Harper

Faculty & Staff Scholarship

Avian monitoring strategies are usually linked to bird singing or calling behavior. Individual availability for detection can change as a result of conspecific factors affecting bird behavior, though the magnitude of these effects is difficult to quantify. We evaluated behavioral and temporal factors affecting Northern Bobwhite (Colinus virginianus) breeding season individual availability for detection during three common survey times (3 min, 5 min, 10 min). We conducted 10-minute surveys associated with radio-collared male Northern Bobwhites on Peabody Wildlife Management Area, Kentucky, from 2010–2011. We homed to within 50 m of radio-collared males and recorded number of distinct Northern …


Analysis Of Transient Hypermorphic Activity Of E(Spl)D During R8 Specification, Adam T. Majot, Ashok P. Bidwai Jan 2017

Analysis Of Transient Hypermorphic Activity Of E(Spl)D During R8 Specification, Adam T. Majot, Ashok P. Bidwai

Faculty & Staff Scholarship

Drosophila atonal (ato) is required for the specification of founding R8 photoreceptors during retinal development. ato is regulated via dual eye-specific enhancers; ato-3’ is subject to initial induction whereas 5’-ato facilitates Notch-mediated autoregulation. Notch is further utilized to induce bHLH repressors of the E(spl) locus to restrict Ato from its initial broad expression to individual cells. Although Notch operates in two, distinct phases, it has remained unclear how the two phases maintain independence from one another. The difference in these two phases has attributed to the hypothesized delayed expression of E(spl). However, immunofluorescence data indicate that E(spl) are expressed during …


Temporal Changes In Sphingolipids And Systemic Insulin Sensitivity During The Transition From Gestation To Lactation, J. Eduardo Rico, Sina Saed Samii, Alice T. Mathews, Jacqueline Lovett, Norman J. Haughey, Joseph W. Mcfadden Jan 2017

Temporal Changes In Sphingolipids And Systemic Insulin Sensitivity During The Transition From Gestation To Lactation, J. Eduardo Rico, Sina Saed Samii, Alice T. Mathews, Jacqueline Lovett, Norman J. Haughey, Joseph W. Mcfadden

Faculty & Staff Scholarship

Reduced insulin action develops naturally during the peripartum to ensure maternal nutrient delivery to the fetus and neonate. However, increased insulin resistance can facilitate excessive lipolysis which in turn promotes metabolic disease in overweight dairy cattle. Increased fatty acid availability favors the accumulation of the sphingolipid ceramide and is implicated in the pathogenesis of insulin resistance, however, the relationship between sphingolipid metabolism and insulin resistance during the peripartum remains largely unknown. Our objectives were to characterize temporal responses in plasma and tissue sphingolipids in lean and overweight peripartal cows and to establish the relationships between sphingolipid supply and lipolysis, hepatic …