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De Novo Co-Assembly Of Bacterial Genomes From Multiple Single Cells, Narjes Sadat Movahedi Tabrizi
De Novo Co-Assembly Of Bacterial Genomes From Multiple Single Cells, Narjes Sadat Movahedi Tabrizi
Wayne State University Theses
Recent progress in DNA amplication techniques, particularly multiple displacement amplication (MDA), has made it possible to sequence and assemble bacterial genomes from a single cell. However, the quality of single cell genome assembly has not yet reached the quality of normal multicell genome assembly due to the coverage bias and errors caused by MDA. Using a template of more than one cell for MDA or combining separate MDA products has been shown to improve the result of genome assembly from few single cells, but providing identical single cells, as a necessary step for these approaches, is a challenge. As a …