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Molecular Mechanisms Of Strain-Stimulated Intestinal Epithelial Cell Differentiation And Migration, Lisi Yuan
Molecular Mechanisms Of Strain-Stimulated Intestinal Epithelial Cell Differentiation And Migration, Lisi Yuan
Wayne State University Dissertations
The intestinal epithelium is subjected to repetitive deformation during normal gut function by peristalsis and villous motility. In vitro, cyclic strain promotes intestinal epithelial proliferation and induces an absorptive phenotype characterized by increased dipeptidyl dipeptidase (DPPIV) expression. Schlafen 3 is a novel gene recently associated with cellular differentiation. We sought to evaluate whether Schlafen 3 mediates the effects of strain on the differentiation of IEC-6 intestinal epithelial cells in the absence or presence of cyclic strain. Strain increased Schlafen 3 mRNA and protein. In cells transfected with a control non-targeting siRNA, strain increased DPPIV specific activity. However, Schlafen
3 reduction …