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Zeta Potential Measurement And Particle Size Analysis For A Better Understanding Of Urinary Inhibitors Of Calcium Oxalate Crystallization, L. C. Cao, G. Deng, E. R. Boeve, W. C. De Bruijn, R. De Water, C. F. Verkoelen, J. C. Romijn, F. H. Schroder
Zeta Potential Measurement And Particle Size Analysis For A Better Understanding Of Urinary Inhibitors Of Calcium Oxalate Crystallization, L. C. Cao, G. Deng, E. R. Boeve, W. C. De Bruijn, R. De Water, C. F. Verkoelen, J. C. Romijn, F. H. Schroder
Scanning Microscopy
To better understand urinary inhibitors of calcium oxalate crystallization, both zeta potential measurement and particle size analysis were chosen to illustrate: (1) the potential therapeutic efficacy of G872, a semi-synthetic sulfated polysaccharide, in stone prevention; and (2) the relative contribution of various urinary fractions {e.g., ultrafiltered urine (UFU), Tamm-Horsfall protein (THP), urinary polyanionsprecipitated with cetylpyridinium chloride (CPC), urinary macromolecular substances with different concentration ratios (UMSl0,50,90 and UMS'l0,50,90) and THP-free urine (THPFU)} to total urinary inhibitory activity. The results showed: (1) addition of G872 significantly enhances urinary inhibitory activity and negative zeta potential values; (2) re-addition of the CPC to UFU …
Heterogeneous Nucleation Of Calcium Oxalate Crystals In Mammalian Urine, Saeed R. Khan
Heterogeneous Nucleation Of Calcium Oxalate Crystals In Mammalian Urine, Saeed R. Khan
Scanning Microscopy
It is generally recognized that calcium oxalate crystal formation in urine is induced by heterogeneous nucleation. However, there is no consensus as to the nature of the nucleation substrate. Evidence is provided in this paper that membranous cellular degradation products are the most likely candidates because they: (1) are ubiquitous in urine and urinary stones; (2) are found in close association with crystal deposits in the kidneys; and (3) can induce nucleation of crystals from a meta-stable solution of calcium oxalate in vitro and metastable urine in vivo.
Etiology Of Calcium Oxalate Nephrolithiasis In Rats. Ii. The Role Of The Papilla In Stone Formation, W. C. De Bruijn, E. R. Boevé, P. R. W. A. Van Run, P. P. M. C. Van Miert, R. De Water, J. C. Romijn, C. F. Verkoelen, L. C. Cao, J. M. Van 'T Noordende, F. H. Schröder
Etiology Of Calcium Oxalate Nephrolithiasis In Rats. Ii. The Role Of The Papilla In Stone Formation, W. C. De Bruijn, E. R. Boevé, P. R. W. A. Van Run, P. P. M. C. Van Miert, R. De Water, J. C. Romijn, C. F. Verkoelen, L. C. Cao, J. M. Van 'T Noordende, F. H. Schröder
Scanning Microscopy
In kidneys of healthy rats submitted to a crystal-inducing diet (CID) with ethylene glycol (EG) and NH4Cl, the fate of retained crystals in the papillar region is studied during a recovery period of one, five or ten days, as model system for human nephrolithiasis. Scanning electron microscopy (SEM) shows, at papillary tips bulging into the calycine space, crystal masses covered either by the epithelium or a thin fibrous veil, or by unidentified mobile cuboidal cells. After CID plus one or five days recovery, small sub-epithelial swellings are seen of large sub-epithelial crystals at or around the papillary tip. …
Experimental Calcium Oxalate Nephrolithiasis And The Formation Of Human Urinary Stones, Saeed R. Khan
Experimental Calcium Oxalate Nephrolithiasis And The Formation Of Human Urinary Stones, Saeed R. Khan
Scanning Microscopy
Calcium oxalate nephrolithiasis in rats requires induction of hyperoxaluria which results in increased urinary calcium oxalate supersaturation. As a result of low to mild chronic hyperoxaluria, calcium oxalate crystals deposit first in the papillary collecting ducts. Crystal deposition in the kidneys is preceded by calcium oxalate crystalluria and starts with the retention of aggregated calcium oxalate crystals in the renal tubules. Retained crystals move from the tubules to the interstitium, and in the process, become anchored to the tubular basement membrane. Crystal aggregates present in the superficial peripheral collecting ducts of the renal papillae ulcerate through to the papillary surface …