Life Sciences Commons^™

Fictitious Calculi And Human Calculi With Foreign Nuclei, P.-T. Cheng, K. P. H. Pritzker, J. Richards, D. Holmyard

Scanning Microscopy

The correlative approach employing polarized light microscopy, x-ray powder diffraction, scanning electron microscopy, transmission electron microscopy, selected area electron diffraction and energy dispersive x-ray microanalysis proves to be very useful in identifying fictitious calculi and genuine human calculi with foreign body nuclei. The common artifacts as reported in the literature and observed also by us were minerals, vegetable and plant seeds, cereals, sand grains and sea shell fragments.

Two interesting cases involving foreign body nuclei have been reported: one urinary calculus containing a piece of plastic-coated titanium foil in the center; one nasal calculus with a nut as a nucleus. …

Effects Of Fractionated Irradiation On The Esophageal Mucosa: A Scanning And Transmission Electron Microscopic Study, M. Albertsson, C-H Hakansson, C. Mercke, H. Morner

Scanning Microscopy

The mucosa of rabbit esophagus was irradiated with daily fractions of 2 Gy to an accumulated dose of 20 Gy. Specimens were taken for scanning electron microscopy, transmission electron microscopy and light microscopy investigations. Examination was made 1-10 days after each fractionation schedule. Light microscopy showed dose-dependent edema of the irradiated mucosa which also could be seen and scored from SEM pictures. SEM investigations showed that this was accompanied by loosening of microridges and a slightly increased cell loss. By SEM, a varying amount of bacteria could be seen which did not make intimate contact with the surface cells.

During …

Effects Of Cis-Dichlorodiammineplatinum Alone And In Combination With Ionizing Radiation On The Esophageal Mucosa: A Scanning And Transmission Electron Microscopic Study, M. Albertsson, C-H Hakansson, C. Mercke

Scanning Microscopy

Cis-dichlorodiammineplatinum (cis-DDP) has for more than 20 years been part of the therapeutic arsenal of oncology. Most of the knowledge about its biological action is based on clinical investigations and therefore an examination of the influence of cis-DDP at the cellular and sub-cellular level is necessary. Five mg of cis-DDP was given intraperitoneally (i.p.) to ten rabbits. Ultrastructural examinations were performed on the upper and lower parts of the esophagus each day after the injection on the following ten days. Another 50 rabbits were given 5 mg cis-DDP and were irradiated in an area just beneath the hypopharynx. They were …

Endothelialization Of A New Dacron Graft In An Experimental Model: Light Microscopy, Electron Microscopy And Immunocytochemistry, G. Pasquinelli, P. Preda, T. Curti, M. D'Addato, R. Laschi

Scanning Microscopy

Two types of synthetic vascular grafts, Dacron Triaxial and Dacron Gelseal Triaxial, were implanted into both the common carotids of sheep. The animals were sacrificed 1, 2, 8, and 16 weeks after surgery. Multiple specimens, obtained from grafts and anastomoses, were studied by light microscopy, transmission and scanning electron microscopy. A parallel immunocytochemical analysis was performed on some specimens. Dacron Triaxial grafts failed to develop a complete neointimal coverage. Myofibroblasts and fibroblasts were the dominant cells in such synthetic graft. Moreover, focal areas of stripping, platelet deposition, and thrombosis were observed at 8 and 16 weeks.

In contrast, a stable …

The Use Of Electron Microscopic Methods For The Characterization Of Paints In Forensic Science, R. Goebel, W. Stoecklein

Scanning Microscopy

Apart from the conventional methods for investigating paints in the forensic science laboratory the electron microscope gives many additional types of information. Since the materials for coating objects are produced in large quantities, a merely chemical analysis of paint does not lead to individual identification. Therefore it is necessary to demonstrate morphological peculiarities, such as e.g., features and defects of fabrication. So the material will have to be evaluated by microanalysis and cathodoluminescence, too. Additionally the pigments and extending materials, especially in primers, can be described by transmission electron microscopy. In that way one can obtain information about the distribution …

Effect Of Ionizing Irradiation And Storage On Mushroom Ultrastructure Ii. The Stipe And The Upper Part Of The Cap Of Agaricus Bisporus (Lge. Imbach), A. Keresztes, E. Kovacs

Food Structure

After having investigated t he ultra structural effects of ionizing irradiation used for shelf -life extension , on the gills of Agaricus bisporus and Pleurotus ostreatus previously , it was of interest to examine how other parts of the fruit body were affected by t he same treatment . Samples were taken from the lower and upper parts of the stipe and from the upper part of t he cap of the control , stored , and irradiate d (2.5 kGy ) t he n stored A. bisporus fruit bodies. Transmission electron microscopy sh owed that irradiated samples generally retained …

Internal Porosity Of Corn Extrudate Air Cell Wall, Samuel H. Cohen, Charles A. Voyle

Food Structure

Transmission electron microscopy was used to observe structures within the air cell wall of a corn-based extrudate. Modified fixation and embedding techniques were employed to obtain optimum thin sections. Photomicrographs from these sections showed minute air cells measuring approximately 5 um or less. The size of air cells might impose a limiting factor on the infusion of particulate materials throughout the extrudate matrix.