Life Sciences Commons^™

Simulation With Pharmacological Agents Of Radiation Damage To Small Intestinal Villi, M. Indran, F. C. Boyle, K. E. Carr

Scanning Electron Microscopy

Irradiation induces damage to intestinal villi, resulting in a progressive decline in villous height and changes in topography. Gamma and neutron radiation are reported to cause changes in the structure of smooth muscle and nerve twigs of the intestinal wall. It is possible, therefore, that villous collapse may be due partly to changes in the underlying stromal elements as a result of damage to nerve or muscle.

To test this hypothesis, mice were treated with the drug reserpine which is known to affect the neural control of intestinal smooth muscle function and the small intestine was examined for topographical and …

Structural Changes In Mouse Small Intestinal Villi Following Lower Body Hyperthermia, H. M. H. Kamel, K. E. Carr, S. P. Hume, J. C. L. Marigold

Scanning Electron Microscopy

Heating an exteriorised loop of mouse small intestine resulted in marked changes in the shape of the villi as reported earlier. However, the exteriorisation techniques resulted in non-uniformity in both temperature and effect around circumference of intestine and, in addition, the extent to which handling contributed to the observed damage was not known. The work has therefore been extended using lower-body heating in the temperature range 37.5° - 43.0°C.

Heating in the temperature range 37.5° to 41.0°C produced minimal to moderate structural changes, manifested as scattered, vertically collapsed villi amongst predominantly "normal" villi. No villi showed conical or rudimentary forms …

In Vivo Light Microscopy Of Organs, Robert S. Mccuskey

Scanning Electron Microscopy

High resolution, brightfield and fluorescence, light microscopic methods have been developed for examining living organs in situ. The methods permit study of the rate, duration, magnitude and direction of dynamic histologic, pathologic, physiologic and pharmacologic events. In addition, morphometric analyses of such living preparations can provide basic information needed to evaluate alterations induced by fixation and processing of these organs for electron microscopy. Most organs are amenable to such investigations. In anesthetized animals, the selected organ is trans-and/or epi-illuminated with selected wavelengths of monochromatic light, imaged with water immersion objectives and the resulting monochromatic optical images televised using silicon …

Comparison Of The Microstructure Of Firm And Stem-End Softened Cucumber Pickles Preserved By Brine Fermentation, W. M. Walter Jr., H. P. Fleming, R. N. Trigiano

Food Structure

Soft and firm stem end tissues of cucumbers fermented at a low concentration of sodium chloride were examined by a combination of light microscopy {LM), transmission electron microscopy { TEM), and scanning electron microscopy { SEM) . When compared to firm tissue, softened tissue cell walls were swollen and striated, and the middle lamellae were poorly stained by the uranyl acetate-1 ead citrate used in TEM . When the tissue was penetrated by a circu l ar punch {1.5 mm diameter), cells in soft tissue tore along the middle l arne ll ae rather than across cell walls, as did …

Effects Of Emulsifying Agents On The Microstructure And Other Characteristics Of Process Cheese - A Review, Marijana Caric, Miroslav Gantar, Miloslav Kalab

Food Structure

Sodium phosphates , polyphosphates, and citrates are melting salts (emulsifying agents) most commonly used in the manufacture of process cheese either alone or in mixtures . Their role during processing is to sequester calcium in the natural cheese, to solubilize protein and increase its hydration and swelling, to facilitate emul sification of fat , and to adjust and stabilize pH.

Changes taking place in natural cheese during processing can be studied by microscopy. Micrographs demonstrating the emulsification of fat , presence of salt crystals, and partial solubilization of protein in labo ratory- made and commercial process cheeses have been used …