Life Sciences Commons^™

Contribution Of Scanning Electron Microscopy And Associated Analytical Techniques To The Study Of Atherosclerotic Disease, R. Laschi

Scanning Electron Microscopy

Human carotid atheroma has been examined by scanning electron microscopy (SEM) utilizing both secondary and backscattered electron detectors and transmission electron microscopy (TEM) both at conventional and high voltage.

Different cytochemical techniques have been used to map elastic fibers, proteoglycans, calcium, non esterified cholesterol. By immunocytochemistry the distribution of factor VIII related antigen and actin has been studied.

With SEM it was possible to detect aspects of carotid plaques not appreciated when using other conventional techniques. With TEM some modifications of the structural and/or functional features of connective tissue macromolecules have been observed. The occurrence of anomalous collagen has been …

Scanning Electron Microscopy Studies Of Staphylococcal Adherence To Heart Valve Endothelial Cells In Organ Culture: An In Vitro Model Of Acute Endocarditis, Morris D. Cooper, Christine Jeffery, Debra L. Gall, Andrew S. Anderson

Scanning Electron Microscopy

Organ cultures of human heart valves were used as a model to study the initial pathobiology of acute infective bacterial endocarditis. We used Staphylococcus aureus isolated from a case of infective endocarditis to infect the in vitro culture of the heart valves. Using scanning electron microscopy, we assessed the initial damage, attachment to and invasion of the endothelial cell layer by staphylococci. Our results indicate there is initial damage to the endothelium prior to observation of staphylococci attaching to the endothelial cell. By 12 h post infection, there is significant attachment and damage. At 24 h after infection, destruction of …

On The Application And Possibilities Of In Vivo Microscopy In Liver Research, E. Wisse, R. S. Mccuskey

Scanning Electron Microscopy

In vivo microscopy (IVM) provides a valuable method for studying the histophysiology of the living liver. The method allows observation of living cells in the intact organ of an anesthetized animal with an undisturbed microcirculation, at a magnification and a resolution comparable to normal light microscopy of sectioned material. Due to the absence of preparative procedures, the image differs substantially from histological sections, but it has the advantage of providing us with a reference preparation free of artifacts. In the case of the liver, we have the opportunity to observe directly such details as bile capillaries, intracellular fat droplets, lysosomes, …