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Full-Text Articles in Life Sciences
Cell Volume Regulation Studies With The Electron Microprobe, Mary Jo Ingram, F. Duane Ingram
Cell Volume Regulation Studies With The Electron Microprobe, Mary Jo Ingram, F. Duane Ingram
Scanning Electron Microscopy
Lumbricals from the hind feet of young rats are dissected free, stretched to approximately 125% of resting length, and mounted on individual, simple plastic forms. After recovery in physiological saline, the isolated muscles are incubated for periods of 40 to 60 min. in one of a series of hypertonic bathing solutions. The composition of each bathing solution is identical, except for osmolality which is increased with lactose. At least one muscle from each animal is incubated in a control solution to serve as a control muscle for that particular set of 5 to 8 muscles. Mounted muscles are removed from …
Two Opposing Theories Of The Cell: Experimental Testing By Cryomethods And Electron Microscopy, L. Edelmann
Two Opposing Theories Of The Cell: Experimental Testing By Cryomethods And Electron Microscopy, L. Edelmann
Scanning Electron Microscopy
A main controversial issue in cell biology concerns the molecular mechanism responsible for K+ accumulation in living cells and Na+ exclusion from them. The alternative theoretical descriptions of these phenomena are based on different assumptions about the physical state of cellular Na+, K+ and H2O. In this article it is shown with striated muscles how cryomethods and microanlytical electron microscopy may be used to test the opposing theories. It is concluded that these methods may yield more realistic informations about the physical state of cellular K+ and Na+ than measurements with …