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Full-Text Articles in Life Sciences

Effect Of Silicon On Plant Growth And Drought Stress Tolerance, Kaerlek W. Janislampi Dec 2012

Effect Of Silicon On Plant Growth And Drought Stress Tolerance, Kaerlek W. Janislampi

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Silicone is a silicon-containing synthetic polymer. Silicon is a metalloid chemical element. Silicon is not considered an essential nutrient for plants, but it is typically abundant in soils and can be taken up in large amounts by plant roots. Silicon is known to have beneficial effects when added to the soil in which rice and several other plants are cultivated. These beneficial effects include disease and insect resistance, plant structural fortification, and regulation of the uptake of other plant nutrients. Silicic acid is the form of silicon in soils that is available to plants. In this study, the effect of …


Factors Influencing Relocation Success Of Utah Prairie Dog (Cynomys Parvidens), Rachel Curtis Dec 2012

Factors Influencing Relocation Success Of Utah Prairie Dog (Cynomys Parvidens), Rachel Curtis

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Utah prairie dogs have been extirpated in 90% of their historic range due to introduced disease, eradication, and habitat destruction. Most of the population lives on private land where animals burrow in lawns and agricultural fields, which keeps this threatened species continually in conflict with landowners. The Utah Division of Wildlife Resources has been relocating prairie dogs from private to public land since the 1970s, but relocations have been largely unsuccessful due to high mortality. Prairie dogs are highly social animals, but they are usually relocated without regard to their family group, or coterie. Coteries typically consist of one reproductive …


Population Dynamics And Movements Of Translocated And Resident Greater Sage-Grouse On Anthro Mountain, Utah, Natasha W. Gruber Dec 2012

Population Dynamics And Movements Of Translocated And Resident Greater Sage-Grouse On Anthro Mountain, Utah, Natasha W. Gruber

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Greater sage-grouse (Centrocercus urophasianus) populations have declined range-wide. Species translocations have been identified as a conservation strategy to augment declining populations in restored habitats. I evaluated protocols previously used to successfully translocate greater sage-grouse in Utah by comparing annual production, survival, habitat use, integration and seasonal movements of translocated birds and their chicks to the resident population. To conduct this study, I translocated 60 greater sagegrouse hens captured and radio-collared on Parker Mountain to Anthro Mountain in the spring of 2009 and 2010. I also captured and radio-collared 19 resident hens to serve as my control group. All …


Modulation Of The Host Response To Tacaribe Arenavirus Infection In Ag129 Mice By My-24, Eric Sefing Dec 2012

Modulation Of The Host Response To Tacaribe Arenavirus Infection In Ag129 Mice By My-24, Eric Sefing

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

MY-24 is a new antiviral compound recently shown to protect immunocompromised mice from lethal challenge with Tacaribe virus (TCRV). Tacaribe virus is incapable of causing disease to humans, but is closely related to the highly pathogenic New World arenaviruses that cause often-fatal viral hemorrhagic fever syndromes. Remarkably, MY-24 prevents mortality without reducing virus burden in the circulation or tissues. To investigate the mechanism by which MY-24 protects AG129 mice against Tacaribe virus infection, we first characterized the natural history of disease in the model with an emphasis on host immune response and blood vessel function to establish the best times …


Using Biophysical Geospatial And Remotely Sensed Data To Classify Ecological Sites And States, Carson A. Stam Dec 2012

Using Biophysical Geospatial And Remotely Sensed Data To Classify Ecological Sites And States, Carson A. Stam

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Within the Intermountain West, vast expanses of big sagebrush shrubland and steppe are considered emblems of the western range. Currently, there are approximately 60 million hectares of big sagebrush within the 11 western states, four million of which are in the state of Utah. However, the historic distribution of sagebrush has been impacted by conversion to other types of land cover through juniper encroachment, urbanization, invasive weeds, and agricultural expansion. In Utah alone, big sagebrush communities have been reduced to approximately 55% of their historic extent. A primary and current example of the cumulative impact of big sagebrush loss is …


Mechanisms And Signal Transduction Pathways Involved In Bovine Oocyte Activation, Ammon Hanson Bayles Dec 2012

Mechanisms And Signal Transduction Pathways Involved In Bovine Oocyte Activation, Ammon Hanson Bayles

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Being able to create a genetically identical, living replicate of a prized animal sounds impossible. Through advanced scientific methods and the wonders of mother nature it can be accomplished by a process called cloning. In 2003, the USU Animal, Dairy, and Veterinary Science department and Dr. Kenneth L. White gained world wide recognition when they, along with a team for Idaho, cloned the first equine species.

Cloning certain animals could be very advantageous to the agricultural industry. Prized animals that have died could be cloned, producing the best meat and milk supply. Animals that are sterile or endangered could be …


Interactions Between Rocky Mountain Bighorn Sheep Ovis Canadensis Canadensis And Domestic Sheep Ovis Aries And The Biological, Social, Economic, And Legal Implications Of These Interactions On Usda Forest Service Lands In The Evanston/Mt. View Ranger District, Ashly Nicole Herrera Aug 2012

Interactions Between Rocky Mountain Bighorn Sheep Ovis Canadensis Canadensis And Domestic Sheep Ovis Aries And The Biological, Social, Economic, And Legal Implications Of These Interactions On Usda Forest Service Lands In The Evanston/Mt. View Ranger District, Ashly Nicole Herrera

All Graduate Plan B and other Reports, Spring 1920 to Spring 2023

Strong evidence exists indicating domestic sheep (Ovis aries) can infect Rocky Mountain bighorn sheep (Ovis canadensis canadensis), a United States Forest Service (USFS) Region 4 sensitive species, with pneumonia (Callan 1991; Foreyt 1989, 1992, 1994; Foreyt and Lagerquist 1996; George et al 2008; Wehausen et al. 2011). Since the transmission of the pneumonic bacteria between the domestic and wild sheep is a result of bighorn sheep coming into contact with the bacteria carried in the mucous membranes of the domestic sheep, bighorn sheep at risk of initially contracting the bacteria are those individuals that enter areas currently being grazed or …


The Effects Of Habitat Manipulations On Utah Prairie Dogs (Cynomys Parvidens) And Their Habitat On The Awapa Plateau Recovery Area In South-Central Utah, Gretchen Elizabeth Caudill Aug 2012

The Effects Of Habitat Manipulations On Utah Prairie Dogs (Cynomys Parvidens) And Their Habitat On The Awapa Plateau Recovery Area In South-Central Utah, Gretchen Elizabeth Caudill

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Utah prairie dogs have been listed, in some capacity, under the Endangered Species Act since 1972. The lack of suitable habitat, particularly cool season grass cover,
has been identified as the primary factor impeding species recovery. Due to reproduction costs, Utah prairie dogs require a large quantity of cool season grasses. Lack of recovery has also been attributed to poor juvenile survival. Increased resources have been shown to increase weights, which would increase survival through the winter.

Beneficial habitat management techniques for Utah prairie dogs have yet to be determined for the Awapa Plateau recovery area. Utah prairie dogs require …


Characterization Of The Involvement Of Integrins, Focal Adhesion Kinase, And Phospholipase C Enzymes Endogenous To The Oocyte In Bovine Fertilization And Oocyte Activation, Benjamin Rand Sessions Aug 2012

Characterization Of The Involvement Of Integrins, Focal Adhesion Kinase, And Phospholipase C Enzymes Endogenous To The Oocyte In Bovine Fertilization And Oocyte Activation, Benjamin Rand Sessions

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The Center for Integrated Biosystems (CIB) and Animal, Dairy, and Veterinary Sciences (ADVS) Department at Utah State University propose a multi-year molecular study of cattle gamete interactions to improve our basic understanding of fertilization. The CIB and ADVS will utilize existing collaborations in addition to recruiting state and federal funding sources to complete the extensive project.

Various laboratory techniques will be used to discover the molecular players of fertilization in hopes of shedding light on a variety of human infertility issues due to problems at the gamete level. A more complete understanding of fertilization could also lead to the development …


Genetic Characterization Of Fine-Leaved Festuca Valesiaca Germplasm And Evaluation Of Their Relationship To The Festuca Ovina Complex, Yingmei Ma Aug 2012

Genetic Characterization Of Fine-Leaved Festuca Valesiaca Germplasm And Evaluation Of Their Relationship To The Festuca Ovina Complex, Yingmei Ma

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Fine-leaved Festuca valesiaca has stress tolerance. However, its agronomic performance in the western United States and its genetic relationship to species of the Festuca ovina complex has not been investigated. Also, natural hybridization makes them difficult to identify. Given the species' potentials, our project was designed to identify Festuca valesiaca accessions that possess high biomass and seed yield and to examine their relatedness with the Festuca ovina complex.

The Festuca valesiaca accessions examined produced many small seeds. Seed production was correlated with the total biomass, plant height, and plant vigor. The Festuca valesiaca accessions examined were shorter than the control …


Effects Of Sodium Chloride Salting And Substitution With Potassium Chloride On Whey Expulsion Of Cheese, Ying Lu Aug 2012

Effects Of Sodium Chloride Salting And Substitution With Potassium Chloride On Whey Expulsion Of Cheese, Ying Lu

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Heart disease and stroke are leading causes of death in America. In 2008, not only are more than 0.6 million people dying of heart disease, but it is estimated that almost 1 in every 4 Americans with heart disease die. Consuming excess salt has been associated with increasing risk of high blood pressure, heart attacks, and stroke. Americans consume 40% more salt than the USDA recommended level -- 1500 mg. Therefore, it is important to reduce the amount of salt in foods such as cheese to reduce salt consumption that may lead to heart disease.

However, simply reducing salt level …


Continental-Scale Characterization Of Molecular Variation In Quaking Aspen, Colin M. Callahan Aug 2012

Continental-Scale Characterization Of Molecular Variation In Quaking Aspen, Colin M. Callahan

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Quaking aspen has the largest natural distribution of any tree native to North America, ranging from Alaska through the breadth of Canada and south to mid-Mexico. Recent studies suggest a general decline of aspen throughout much of the range since at least the mid-20th century, though these findings remain inconclusive. Regardless, factors such as climate change, periods of drought, soil nutrient deficiencies, pathogens, insects, and encroachment by other tree species all pose risks to the health and maintenance of aspen across the continent. This situation is exemplified in the western United States where climate change is forecasted to have an …


Caehnorhabditis Elegans: A Low-Cost In Vivo Animal Model For Efficacy Studies Of Novel Antibiotics, Rylee Ann Gregory Jun 2012

Caehnorhabditis Elegans: A Low-Cost In Vivo Animal Model For Efficacy Studies Of Novel Antibiotics, Rylee Ann Gregory

Undergraduate Honors Capstone Projects

Since the 1940s, antibiotics have greatly reduced the adverse effects of infectious diseases caused by microbes. However, due to excessive, and often incorrect, use of known antibiotics, many organisms have adapted antibiotic resistance. Currently, over 70% of known infectious bacteria are resistant to at least one antibiotic. In the U.S. , 90,000 deaths occur each year due to infection by bacteria resistant to antibiotics. This number has increased by nearly 75,000 in the last 20 years. It is necessary, therefore, to continue developing new antibiotics in an effort to keep up with increasing antibiotic resistance. Traditional in vitro and whole …


Locking Down The N-Terminus Of Prmt1 In Order To Assess The Role Of Motion In Activity, Taylor James Rasmussen May 2012

Locking Down The N-Terminus Of Prmt1 In Order To Assess The Role Of Motion In Activity, Taylor James Rasmussen

Undergraduate Honors Capstone Projects

Protein arginine methyltransferases (PRMTs) are involved In many major biological pathways in the human body. Processes that demonstrate arginine methylation by PRMTs include, but are not limited to, histone modification, DNA transcription, and post-translational protein modifications. Although recent research has allowed the identification of several PRMT isoforms and exposed their involvement in these processes, relatively little is known about the details of how these enzymes perform their biochemical duties. It is currently hypothesized that the N-terminus of PRMT variant I is involved in recognizing substrates and aiding in catalysis by virtue of a change in its conformation. To understand how …


Determining The Effect Of One-On-One Education In Addition To Written Material On Breastfeeding Initiation Rates In The Hospital Setting, Carly Elizabeth Grace May 2012

Determining The Effect Of One-On-One Education In Addition To Written Material On Breastfeeding Initiation Rates In The Hospital Setting, Carly Elizabeth Grace

All Graduate Plan B and other Reports, Spring 1920 to Spring 2023

The objective of this research was to determine the breastfeeding initiation rate among healthy, term infants at a select hospital, the effect of one-on-one breastfeeding education, in addition to written material on breastfeeding initiation rates in the hospital setting, and if other factors from available data are associated with differences in breastfeeding initiation. This was a quasi-experimental convenience sample study with subjects assigned to a control group (written education) or intervention group (written and one-on-one education). The setting was the obstetrics unit at Siloam Springs Memorial Hospital (Benton County, Arkansas). Subjects were women delivering infants without complication between March and …


The Effectiveness Of An Adapted Snap-Ed (Supplemental Nutrition Assistance Program-Education) Curriculum For Adults With Intellectual Or Developmental Disabilities, Amanda Panting May 2012

The Effectiveness Of An Adapted Snap-Ed (Supplemental Nutrition Assistance Program-Education) Curriculum For Adults With Intellectual Or Developmental Disabilities, Amanda Panting

All Graduate Plan B and other Reports, Spring 1920 to Spring 2023

Rates of overweight and obese adults with intellectual or developmental disabilities in the United States are high and associated with increased risk of chronic diseases. Many of these adults are trying to become more independent in the community and live in group homes where care is managed by a paid employee. Group home managers assist clients with daily living such as shopping and meal preparation and play an important role in their care. Nutrition and healthy behavior choices are important to help keep this population independent and decrease risks of excess weight and related diseases. Many group home managers and …


Landscape And Habitat Attributes Influencing Beaver Distribution, Ryan John Leary May 2012

Landscape And Habitat Attributes Influencing Beaver Distribution, Ryan John Leary

All Graduate Plan B and other Reports, Spring 1920 to Spring 2023

Removal of beaver across the North America landscape from the 1600s through the 1800s has played a major influence on the alteration of stream and riparian resources. Degradation of riparian habitats has negatively impacted many wildlife and fish species, including species listed under the Endangered Species Act. The ability of beavers to modify stream ecosystems offers a unique opportunity to restore these habitats. Many private and government agencies are working towards using beaver as a restoration tool, not only for better functioning ecosystems but also to benefit humans. Taking the big picture look, beaver and their ability to modify the …


Development Of Methods For Assessing The Effect Of Moisture And Aging On Sliceability Of Cheese, Jess Perrie May 2012

Development Of Methods For Assessing The Effect Of Moisture And Aging On Sliceability Of Cheese, Jess Perrie

All Graduate Plan B and other Reports, Spring 1920 to Spring 2023

Sliceability is a cheese’s ability to cut cleanly into thin slices, resist breakage or fracture at slices edges, and undergo a high level of bending before breaking. Intuitively, sliceability depends on the chemistry, microstructural, and rheological properties of the casein network. Currently there is no reported scientific research investigating evaluation methods of cheese slice quality, as well as properties that influence a cheese’s ability to slice.

In this study, a method for slice quality evaluation was developed on purchased cheese and performed on commercial cheeses and experimental cheeses manufactured at three different moisture contents (40.6%, 37.0%, and 33.9%). In addition, …


Preschool Nutrition Education And Influences On Food Neophobia, Kelsey Eller May 2012

Preschool Nutrition Education And Influences On Food Neophobia, Kelsey Eller

All Graduate Plan B and other Reports, Spring 1920 to Spring 2023

Food neophobia, the fear of new foods, has been identified as a significant barrier to the intake of healthy foods, including fruits and vegetables in young children. Food neophobia can hinder dietary quality as well as dietary variety by limiting the development of food preferences. It has also been suggested that neophobia is linked to the development of childhood obesity, and due to the escalating epidemic of childhood obesity that is known to be associated with serious health complications, interventions that target food neophobia in preschool aged children may be successful in improving healthy eating habits and potentially reversing the …


The Role Of Fine-Scale Habitat Associations In Structuring Spider Assemblages: Determinants Of Spatial Patterns In Community Composition, Stephanie M. Cobbold May 2012

The Role Of Fine-Scale Habitat Associations In Structuring Spider Assemblages: Determinants Of Spatial Patterns In Community Composition, Stephanie M. Cobbold

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Ecologists strive to identify the mechanisms that drive the identity and abundance of species in different locations, because a better understanding of such factors enables them to better predict the effects of habitat modification on organisms, and to identify landscapes in which species are likely to benefit from conservation interventions. However, there is still no consensus on the mechanisms behind geographical variation in species diversity. The primary objective of this dissertation was to focus on spider assemblages to investigate how the fine-scale habitat associations of organisms may drive the composition of their communities at larger scales. Research was conducted in …


Ensuring Microbial Safety In Food Product/Process Development: Alternative Processing Of Meat Products And Pathogen Survival In Low-Salt Cheddar Cheese, Subash Shrestha May 2012

Ensuring Microbial Safety In Food Product/Process Development: Alternative Processing Of Meat Products And Pathogen Survival In Low-Salt Cheddar Cheese, Subash Shrestha

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Most outbreaks of foodborne illness in the United States occur as a result of improper food-handling and preparation practices in homes or food establishments. The lack of food-safety knowledge is one of the several reasons for this. However, researchers also suggest that food-operators and consumers with adequate food-safety knowledge, attitudes, and intentions do not always follow the food-safety recommendations because not all recommendations are easy to put into practice. Therefore, the first part of this study sought to establish safe alternative processing of meat products that can be easily practiced by food-operators and consumers. In Chapter 3, a novel method …


Clark's Nutcracker Seed Harvest Patterns In Glacier National Park And A Novel Method For Monitoring Whitebark Pine Cones, Monika E. Maier May 2012

Clark's Nutcracker Seed Harvest Patterns In Glacier National Park And A Novel Method For Monitoring Whitebark Pine Cones, Monika E. Maier

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Clark's Nutcracker (Nucifraga columbiana) is the primary seed disperser of whitebark pine (Pinus albicaulis), which is in decline throughout its range. There is concern that a decline in whitebark pine will lead to a subsequent decline in local populations of Clark's Nutcracker. Because natural regeneration depends on the presence of Clark's Nutcracker, the process of harvesting whitebark pine seeds needs to be fully understood. In addition, resource managers need a cost-effective method for monitoring nutcracker occurrence in whitebark pine stands during the seed harvest season. I visited eleven study sites in Glacier National Park, Montana, where …


Increasing The Retention Of Lipid-Soluble Components In A Curd Matrix, Megan Tippetts May 2012

Increasing The Retention Of Lipid-Soluble Components In A Curd Matrix, Megan Tippetts

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Megan Tippetts, under the direction of Dr. Silvana Martini at Utah State University, proposes to demonstrate that it is possible to increase the retention of lipid-soluble components (e.g. vitamin D) in a curd matrix (e.g. Cheddar cheese). This project was coordinated with the Western Dairy Center for the initial phase of the project.

The initial phase used vitamin D3 fortified emulsions stabilized with a dairy-based emulsifier. The objective was to determine if vitamin D3 delivered in an emulsion form to the milk before cheesemake would have higher retention in the cheese versus vitamin D3 oil added directly …


Parent Nutrition Education And The Influence On Family Lifestyle Behavior Changes, Kelsey Rich May 2012

Parent Nutrition Education And The Influence On Family Lifestyle Behavior Changes, Kelsey Rich

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Utah State University in the collaboration with the Supplemental Nutrition Assistance Program-Education (SNAP-Ed) proposes to develop and implement a program to help families develop healthy eating habits and lifestyle behaviors. Recently, childhood overweight and obesity has reached epidemic proportions. The diseases associated with adult obesity are now being seen in the pediatric population; therefore, there is a call for preventative efforts. A diet high in fruits, vegetables, and whole grains in combination with an active lifestyle discourages the presence of obesity. Currently, most childhood obesity prevention efforts have taken place in the school setting and have only had short term …


Caddisfly Larvae (Limnephilidae) As Predators Of Newt (Taricha Granulosa) Eggs: Another Player In The Coevolutionary Arms Race Revolving Around Tetrodotoxin?, Brian G. Gall May 2012

Caddisfly Larvae (Limnephilidae) As Predators Of Newt (Taricha Granulosa) Eggs: Another Player In The Coevolutionary Arms Race Revolving Around Tetrodotoxin?, Brian G. Gall

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Some populations of newts (Taricha granulosa) possess large quantities of the neurotoxin tetrodotoxin (TTX) in their skin and eggs. Many populations of garter snake (Thamnophis sirtalis) are resistant to this toxin and can consume large numbers of newts with no negative effects. Despite the wealth of information acquired on the interaction between newts and their predator, garter snakes, very little research has been conducted on possible interactions between newts and other predators. I conducted a suite of experiments examining for the presence of other predators on newts, specifically focusing on predators of their eggs and larvae. …


Identifying And Quantifying Sediment Sources And Sinks In The Root River, Southeastern Minnesota, Justin Collin Stout May 2012

Identifying And Quantifying Sediment Sources And Sinks In The Root River, Southeastern Minnesota, Justin Collin Stout

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Fine sediment, normally understood to refer to sand, silts, and clays; is a natural constituent in all rivers. When in excess this fine sediment can degrade the habitat for aquatic life in these rivers, and carry with it many pollutants and nutrients which can cause adverse effects on wildlife and human populations.

Understanding how this fine sediment moves through a river system, from erosion off of hillslopes, transport through the river, and finally export from the mouth of the system is vital part to land and river management. However, predicting how fine sediment moves through a system is a difficult …


Genetic Diversity And Genetic Structuring At Multiple Spatial Scales Across The Range Of The Northern Leopard Frog, Rana Pipiens, Ryan P. O'Donnell May 2012

Genetic Diversity And Genetic Structuring At Multiple Spatial Scales Across The Range Of The Northern Leopard Frog, Rana Pipiens, Ryan P. O'Donnell

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Genetic diversity is the raw material for evolution: evolution cannot happen without genetic diversity, and the ability of a population to respond to a changing environment depends directly on how diverse its genes are. Understanding the distribution of genetic diversity is important for many reasons, including predicting whether species will be able to adapt to climate change and predicting the spread of invasive species. Information about the distribution of genetic diversity across the range of the Northern Leopard Frog, a declining species, will not only help us to ensure that the species can continue to evolve in response to changing …


Modeling Freshwater Mussel Distribution In Relation To Biotic And Abiotic Habitat Variables In The Middle Fork John Day River, Oregon, Ericka E. Hegeman May 2012

Modeling Freshwater Mussel Distribution In Relation To Biotic And Abiotic Habitat Variables In The Middle Fork John Day River, Oregon, Ericka E. Hegeman

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Freshwater mussels are the most threatened taxonomic group in North America with extinction rates that exceed those of many species found in both terrestrial and freshwater ecosystems including fish, birds, and amphibians. Part of the reason that mussels are so threatened is because their larvae are parasitic on fish, making the completion of their life cycle dependent upon healthy fish populations. The imperilment of freshwater mussels is a cause for concern because of the benefits that mussels provide to freshwater ecosystems including habitat enhancement, substratum stabilization, nutrient cycling, and water clarification. Restoration and conservation efforts targeting western freshwater mussels have …


1. Improving The Yield Of Biodiesel From Microalgae And Other Lipids. 2. Studies Of The Wax Ester Biosynthetic Pathway And Potential Biotechnological Application, Bradley D. Wahlen May 2012

1. Improving The Yield Of Biodiesel From Microalgae And Other Lipids. 2. Studies Of The Wax Ester Biosynthetic Pathway And Potential Biotechnological Application, Bradley D. Wahlen

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The production of biofuels and oleochemicals from renewable sources offers an opportunity to reduce our dependence on fossil fuels. The work contained in this dissertation has focused on developing and improving methods for the production of biodiesel from non-traditional feedstocks and understanding biosynthetic pathways that result in the production of oleochemicals and fuels.

Pure vegetable oil can account for 70-80% of the total cost of biodiesel production. Many low-cost oils contain high amounts of free fatty acids, which are unsuitable for base-catalyzed transesterification. Herein an approach is described that efficiently accomplishes the simultaneous esterification and transesterification of both free fatty …


Productivity Studies Utilizing Recombinant Cho Cells In Stirred-Tank Bioreactors: A Comparative Study Between The Pitch-Blade And The Packed-Bed Bioreactor Systems, Taylor Stephen Hatton May 2012

Productivity Studies Utilizing Recombinant Cho Cells In Stirred-Tank Bioreactors: A Comparative Study Between The Pitch-Blade And The Packed-Bed Bioreactor Systems, Taylor Stephen Hatton

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The Center for Integrated Biosystems (CIB) at Utah State University proposed to demonstrate the added benefits of bench top stirred-tank bioreactors utilizing the packedbed impeller system in perfusion mode for the production of recombinant proteins. In this study recombinant Chinese Hamster Ovary (CHO) cells were utilized for the production of a secreted protein in two bioreactor types: pitched-blade bioreactor operated in batch mode versus packed-bed bioreactor operated in perfusion mode. Our primary objective was to determine whether the packed-bed bioreactor is superior to a traditional pitched-blade bioreactor for the mass production of cell culture protein products. We hypothesize that the …