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Interaction Of The Human Serine Protease Inhibitor Alpha-1-Antitrypsin With Cryptosporidium Parvum, John Russell Forney
Interaction Of The Human Serine Protease Inhibitor Alpha-1-Antitrypsin With Cryptosporidium Parvum, John Russell Forney
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
The human serine protease inhibitor (serpin) alpha-1-antitrypsin (AAT) was studied for potential interaction with components of the protozoan parasite Cryptosporidium parvum. A homogenate prepared from C. parvum oocysts was incubated with purified human AAT, and complexes formed between the serpin and components of the homogenate were detected using an enzyme-linked immunosorbent assay (ELISA). Serpin:parasite infections were effectively blocked by preincubating AAT with a cognate target enzyme, porcine pancreatic elastase, prior to performing the ELISA on the homogenate. Incubation of a mixture of C. parvum oocysts and sporozoites with AAT demonstrated preferential fluorescence labeling of the sporozoite surface membrane by indirect …