Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 2 of 2
Full-Text Articles in Life Sciences
Development Of Fluorescent In Situ Hybridization Techniques For Physical Mapping In Ovis Aries, Eleanor P. Jenson
Development Of Fluorescent In Situ Hybridization Techniques For Physical Mapping In Ovis Aries, Eleanor P. Jenson
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Genome maps provide information used to identify economic trait loci and loci proximal to genes affecting economically important traits. This research contributes to the development of the ovine genome map by establishing ovine tissue culture techniques and fluorescent in situ hybridization techniques in this laboratory. In addition, a karyotype for sheep affected with spider lamb syndrome was developed. Ovine tissue culture techniques were established using fibroblasts grown from primary explants of sheep and modified cytogenetic procedures. Metaphase chromosomes were obtained from the fibroblast cultures and used for fluorescent in situ hybridization and for banding procedures to develop the spider lamb …
Effects Of Iron Fortification On Microbiological, Physical, Chemical, And Organoleptic Properties Of Yogurt, Sharareh Hekmat
Effects Of Iron Fortification On Microbiological, Physical, Chemical, And Organoleptic Properties Of Yogurt, Sharareh Hekmat
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
It has been shown that iron binds strongly to the proteins in milk, and our aim was to determine whether or not this binding was affected by lowering pH in the manufacture of yogurt. Iron-protein complexing was studied using two different techniques. 1) Skim milk was fortified with 10 mg iron/100 ml and the pH of the milk was adjusted to 6.7, 6.2, 5.8, 5.3, 4.5, and 4.0. The milk was fractionated by ultracentrifugation at 52,000 x g for 60 minutes. The pellets and serum were then analyzed for iron, calcium, and phosphorus content by inductively coupled plasma spectroscopy. SDS-PAGE …