Open Access. Powered by Scholars. Published by Universities.®
- Discipline
Articles 1 - 2 of 2
Full-Text Articles in Life Sciences
In Vitro Evaluation Of Anti-Eimeria Tenella, Gamont-Specific, Monoclonal Antibodies And Partial Characterization Of Their Target Antigens, Eric Wilson
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
This study represents an effort to interrupt the life cycle of Eimeria tenella, the parasite that causes cecal coccidiosis in chickens, with a panel of 12 gamont-specific monoclonal antibodies (Mabs). To facilitate Mab screening, it was first necessary to develop a cell culture-adapted strain of E. tenella (field strain 80) from primary chicken kidney cells (PCKC) to white Leghorn chickens and back to PCKC. This alternation was repeated through 12 such passages. As a result, we have developed a cell culture-adapted strain of E. tenella that produces over 280% more oocysts in vitro than the original parent strain, and …
Effects Of Antioxidants On Development Of In Vitro Fertilized Bovine Embryos, Bret L. Anderson
Effects Of Antioxidants On Development Of In Vitro Fertilized Bovine Embryos, Bret L. Anderson
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Free radicals are short-lived molecules that can cause decreased embryonic development in vitro. Antioxidants are molecules that block free radical formation or guard against their harmful effects. Many studies have linked exposure of media to light and culturing of embryos in high (20%) oxygen concentrations to free radical production. Some of the antioxidants used in culture media are superoxide dismutase (SOD), catalase, zinc (II), ethylenedinitrilo tetraacetic acid (EDTA), mannitol, vitamin E, dimethyl sulfide, and taurine. Most research involving antioxidants and embryonic development has been conducted on non-farm animals, particularly mouse and rabbit. Studies have shown that antioxidants in vitro culture …