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Functional Study Of The Structural Vp6 Protein Of Bluetongue Virus, Emiko Hayama
Functional Study Of The Structural Vp6 Protein Of Bluetongue Virus, Emiko Hayama
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
This study was undertaken to investigate the structure-function relationship of VP6 protein of bluetongue virus (BTV) using molecular cloning techniques. VP6 is present in small quantities in BTV and its enzymatic activity and role in the viral replication cycle have not been studied. Since the availability of large amounts of purified VP6 is essential for the analysis of VP6, a BTV-11 S3 gene was cloned into a prokaryotic protein expression system. VP6 protein was expressed in large amounts and purified to near homogeneity. A series of C-terminal and internal deletion mutants of S3 gene was constructed and the truncated VP6 …