Open Access. Powered by Scholars. Published by Universities.®

Life Sciences Commons

Open Access. Powered by Scholars. Published by Universities.®

Utah State University

Journal

1990

Fixation

Articles 1 - 3 of 3

Full-Text Articles in Life Sciences

Preparation Of Cereals And Grain Products For Transmission Electron Microscopy, Donald B. Bechtel Jan 1990

Preparation Of Cereals And Grain Products For Transmission Electron Microscopy, Donald B. Bechtel

Food Structure

This tutorial specifically addresses the techniques used in the processing of cereals and grain products for various aspects of transmission electron microscopy. Methods covered include sample treatment, chemical and physical fixation, dehydration, embedding, sectioning techniques, immunocytochemistry, enzymatic digestions, carbohydrate localization, and lectin binding. The primary goal is to provide information on the preparation of cereals and cereal-based products for microscopic analysis and to assist the reader in solving technical problems associated with studying cereals or other difficult-to-prepare samples.


A Method For The Examination Of The Microstructure Of Stabilized Peanut Butter, Clyde T. Young, William E. Schadel Jan 1990

A Method For The Examination Of The Microstructure Of Stabilized Peanut Butter, Clyde T. Young, William E. Schadel

Food Structure

A method for light and scanning electron microscopy of damaged resting peanut seed tissue was adapted as a research tool for evaluating the microstructural features of commercially available stabilized peanut butter. This method was used in the present study to evaluate the degree of homogenization of stabilized peanut butter by examining the spatial relationship which exists among the microstructural features. Light and scanning electron microscopy of three commercially available stabilized peanut butters revealed varying degrees of homogenization of broken cell and tissue fragments, protein bodies, and starch grains within a matrix of stabilized oil.


Transmission And Scanning Electron Microscopy Of Peanut (Arachis Hypogaea L. Cv. Florigiant) Cotyledon After Roasting, Clyde T. Young, William E. Schadel Jan 1990

Transmission And Scanning Electron Microscopy Of Peanut (Arachis Hypogaea L. Cv. Florigiant) Cotyledon After Roasting, Clyde T. Young, William E. Schadel

Food Structure

Changes in the microstructure of peanut (Arachis~ L. cv. Florigiant) cotyledon after roasting at a temperature of 160°C for 16 minutes were investigated with transmission and scanning electron microscopy. Thermal modifications were documented with photomicrographs of t he cytoplasmic network , protein bodies, starch grains and cell-to-cell junctions after oven roasting. These thermal modifications include disruption of the cytoplasmic network, distension of protein bodies, decreased stain affinity of starch grains , and disintegration of middle lamellae in some cell-t o-cell junctions.