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Full-Text Articles in Life Sciences
Morphological Abnormalities In Vitamin B6 Deficient Tarsometatarsal Chick Cartilage, P. G. Masse, V. E. Colombo, F. Gerber, D. S. Howell, H. Weiser
Morphological Abnormalities In Vitamin B6 Deficient Tarsometatarsal Chick Cartilage, P. G. Masse, V. E. Colombo, F. Gerber, D. S. Howell, H. Weiser
Scanning Microscopy
The aim of this study was to test the hypothesis that deficiency of vitamin B6 would produce morphological characteristics of osteochondral lathyrism. To accomplish this goal, morphological characteristics of chick cartilage in which lathyrism was produced by two separate dietary regimens was compared to morphological changes encountered in vitamin B6 deficiency. Vitamin B6 deficiency should reduce activity of lysyloxidase needed for producing intermolecular cross-links. The question to be addressed was: would this latter deficiency impair collagen morphological features and secondarily other structures indirectly by reducing collagen molecular assembly? Failure of cross-linking of collagen in the positive controls …
A Low Temperature Vacuum Embedding Procedure For X-Ray Microanalysis Of Biological Specimens At Subcellular Level, Romuald Wroblewski, Joanna Wroblewski, Sven-Olof Wikström, Matti Anniko
A Low Temperature Vacuum Embedding Procedure For X-Ray Microanalysis Of Biological Specimens At Subcellular Level, Romuald Wroblewski, Joanna Wroblewski, Sven-Olof Wikström, Matti Anniko
Scanning Microscopy
The validity of freeze-drying and low temperature embedding in Lowicryl resins has been investigated in studies of ion distribution in mouse embryological inner ear, rat rib growth plate, liver and submandibular gland. The morphological preservation of the tissues was adequate for the identification of different intracellular compartments and extracellular structures. It was also possible to analyze extracellular fluids in the vestibular part of the developing inner ear. Compared with thin cryosections, Lowicryl sections are easier to produce and are more stable during analysis. Freeze-dried embedded material can be easily reorientated during cutting and adjacent sections can be used for other …