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A Cytochemical Scanning Electron Microscopy Study Of Non-Specific Acid Esterase And Acid Phosphatase Activities In Human Peripheral Blood Lymphocytes, A. Campos, E. Fernández-Segura, J. M. García-López
A Cytochemical Scanning Electron Microscopy Study Of Non-Specific Acid Esterase And Acid Phosphatase Activities In Human Peripheral Blood Lymphocytes, A. Campos, E. Fernández-Segura, J. M. García-López
Scanning Microscopy
We analyzed the distribution patterns of nonspecific acid esterase and acid phosphatase activities with cytochemistry-scanning electron microscopy in backscattered and secondary electron imaging modes in isolated normal human peripheral blood lymphocytes . The analysis of non-specific acid esterase activity in the backscattered electron imaging mode showed, in some cells, focal distribution with a well-defined, homogenous deposit. Two patterns of acid phosphatase activity were evident with the backscattered electron imaging mode, i.e., focal and granular. Peripheral blood lymphocytes showing focal activity of both enzymes presented a smooth surface with few scattered microvilli as seen with the secondary electron imaging mode ; …
Dose- And Time-Dependent Increase Of Lysosomal Enzymes In Embryonic Cartilage In Vitro After Ionizing Radiation, M. Cornelissen, L. De Ridder
Dose- And Time-Dependent Increase Of Lysosomal Enzymes In Embryonic Cartilage In Vitro After Ionizing Radiation, M. Cornelissen, L. De Ridder
Scanning Microscopy
Radiation doses of 20, 50 or 100 Gy caused the same time related decrease for RNA and proteoglycan (PG) synthesis in embryonic cartilage in vitro (4 days culture). In this paper, participation of lysosomes in this radiation response is investigated. Therefore, we employ a cytochemical method using 𝜷-glycerophosphate as substrate for acid phosphatase (AP) detection. Increase of AP was found 2 days after irradiation and increased during the whole culture period. The increase was more pronounced with a higher radiation dose. Stimulation of AP activity explains the observed radiation response of RNA and PG synthesis.