Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 2 of 2
Full-Text Articles in Life Sciences
Cryoultramicrotomy And Immunocytochemistry In The Analysis Of Muscle Fine Structure, L. -E. Thornell, G. S. Butler-Browne, E. Carlsson, H. M. Eppenberger, D. O. Fürst, B. K. Grove, B. Holmbom, J. V. Small
Cryoultramicrotomy And Immunocytochemistry In The Analysis Of Muscle Fine Structure, L. -E. Thornell, G. S. Butler-Browne, E. Carlsson, H. M. Eppenberger, D. O. Fürst, B. K. Grove, B. Holmbom, J. V. Small
Scanning Electron Microscopy
Cryoultramicrotomy, which avoids the use of harsh fixation procedures, deleterious dehydration and plastic embedding can be combined with immunocytochemis try to determine the ultra-structural localization of cellular proteins. Our attempts to use the cryosectioning technique in combination with immunolabelling to bridge the gap between light and electron microscopic analysis of muscle morphology have enabled us to obtain new information on fibre typing at the ultrastructural level. Furthermore, we have obtained a marked improvement in the resolution of myofibrillar structures by using semithin cryosections for fluorescence microscopy. Data are also presented on correlated light and electron microscope immunocytochemistry of myocardial intermediate …
Scanning Electron Microscopical And Histochemical Study Of The Endoderm In The Early Chick Embryo, Marjorie A. England, Jennifer Wakely
Scanning Electron Microscopical And Histochemical Study Of The Endoderm In The Early Chick Embryo, Marjorie A. England, Jennifer Wakely
Scanning Electron Microscopy
The endoderm of gastrulating chick embryos shows regional variations in cell shape and size. These were studied by scanning electron microscopy, histochemistry and immunofluorescence. Particular attention was given to the distribution of the cytoskeleton. Four zones of differing morphology were observed. The changing size and shape of these zones could be correlated with the entry of the definitive endoblast through the primitive streak, displacing existing primary hypoblast to the edges of the area pellucida. Endodermal cells were shown to have a well organised cytoskeleton. The cytoskeletons of individual cells were linked to give a cytoskeletal network extending across the endoderm …