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Sequenced-Based Paternity Analysis To Improve Breeding And Identify Self-Incompatibility Loci In Intermediate Wheatgrass (Thinopyrum Intermedium), Jared Crain, Steve Larson, Kevin Dorn, Traci Hagedorn, Lee Dehaan, Jesse Poland
Sequenced-Based Paternity Analysis To Improve Breeding And Identify Self-Incompatibility Loci In Intermediate Wheatgrass (Thinopyrum Intermedium), Jared Crain, Steve Larson, Kevin Dorn, Traci Hagedorn, Lee Dehaan, Jesse Poland
Forage and Range Research Laboratory Publications
Key Message
Paternity assignment and genome-wide association analyses for fertility were applied to a Thinopyrum intermedium breeding program. A lack of progeny between combinations of parents was associated with loci near self-incompatibility genes.
Abstract
In outcrossing species such as intermediate wheatgrass (IWG, Thinopyrum intermedium), polycrossing is often used to generate novel recombinants through each cycle of selection, but it cannot track pollen-parent pedigrees and it is unknown how self-incompatibility (SI) genes may limit the number of unique crosses obtained. This study investigated the potential of using next-generation sequencing to assign paternity and identify putative SI loci in IWG. Using …