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The Effect Of Bacterial Endotoxin Upon The Morphology Of The Tectorial Membrane And Stereocilia In The Guinea Pig Cochlea, S. D. Comis, M. P. Osborne, M. J. Tarlow
The Effect Of Bacterial Endotoxin Upon The Morphology Of The Tectorial Membrane And Stereocilia In The Guinea Pig Cochlea, S. D. Comis, M. P. Osborne, M. J. Tarlow
Scanning Microscopy
Endotoxin of E coli was microperfused into scala tympani or injected into the cerebrospinal fluid in anaesthetised pigmented guinea pigs. The effects of endotoxin on the cochlea were studied using electrophysiological techniques and scanning electron microscopy. We found a drop in the amplitude of the cochlear microphonics and compound action potentials 2 to 2.5 hours after injection. There were also changes in the morphology of stereocilia and the tectorial membrane. The stereocilia lost their rigidity and the tectorial membrane appeared swollen. These effects were less severe in animals which were pretreated with dexamethasone.
Three Dimensional Intracellular Structure Of The Cochlea Using The A-O-D-O Method, A. Nagasawa, R. V. Harrison, R. J. Mount, Y. Harada
Three Dimensional Intracellular Structure Of The Cochlea Using The A-O-D-O Method, A. Nagasawa, R. V. Harrison, R. J. Mount, Y. Harada
Scanning Microscopy
We have observed the three-dimensional intracellular structure of the organ of Corti and the spiral ganglion of the chinchilla using the aldehyde osmium-DMSO-osmium (A-O-D-O) method, and compared our findings with previous studies in the guinea pig. In cells of the spiral ganglion and the organ of Corti, microtubules and other cytoplasmic filaments were digested by OsO4 during processing to provide a clear view of membranous structures such as mitochondria, endoplasmic reticulum and Golgi apparatus. The appropriate digestion time with 0.1% OsO4 solution was found to be 60-80 hrs for the organ of Corti and 70-90 hrs for the …
Localisation Of Putative Mechanoelectrical Transducer Channels In Cochlear Hair Cells By Immunoelectron Microscopy, Carole M. Hackney, David N. Furness, Dale J. Benos
Localisation Of Putative Mechanoelectrical Transducer Channels In Cochlear Hair Cells By Immunoelectron Microscopy, Carole M. Hackney, David N. Furness, Dale J. Benos
Scanning Microscopy
Displacement of the apical stereociliary bundle of cochlear hair cells mechanically gates transducer channels. Knowing the position of the channels with regard to the apical structures of the hair cell could indicate how this mechanism operates. At present, there is conflicting evidence regarding their precise location; the channels have been suggested to be located either towards the base of the stereocilia or at the tips where they could be operated by extracellular links running from the top of shorter stereocilia to the sides of adjacent taller ones. The channels have been shown to be reversibly blocked by amiloride. This has …