Life Sciences Commons^™

Glycerol Permeability In Erythrocytes Of Peromyscus Californicus : The Effect Of Temperature, James K. Vanarsdel

University of the Pacific Theses and Dissertations

The present work was done to determine the effect of temperature of the half-saturation constant (ø) and the maximum transport rate (K) of the facilitated diffusion of glycerol across the erythrocyte membrane of Peromyscus californicus.

Facilitated Diffusion In Rabbit Erythrocytes, Arthur Hing-Cheung Chui

University of the Pacific Theses and Dissertations

The present kinetic study of the permeability of rabbit erythrocytes has established that carrier systems are involved in the penetration of certain non-electrolytes. Saturation, competitive inhibition, and butanol inhibition kinetics were used to demonstrate the presence of carrier systems and the values of half-saturation constants (ø) were determined for the following water soluble non-electrolytes: glycerol, ethylene glycol, urea, and thiourea.

These non-electrolytes are commonly used in permeability studies because they are relatively non-toxic and their small sizes allow penetration of the erythrocyte membrane within a reasonable length of time.

Possible Carriers In Mouse Erythrocytes, Joseph Doyle Mcclure

University of the Pacific Theses and Dissertations

Early qualitative erythrocyte permeability studies (Gryns, 1896; Hedin, 1897) revealed the wide range of non-electrolyte permeability rates across a cell membrane composed of lipid molecules. Theoretically, water-soluble non-electrolytes such as glycerol, ethylene glycol and erythritol would penetrate less rapidly than lipid-soluble non-electrolytes. It was thought that the rate of penetration of water-soluble non-electrolytes was inversely proportional to their molecular size and the penetration of lipid-soluble non-electrolytes was directly proportional to their lipid-solubility.