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Full-Text Articles in Life Sciences
Does Cytotoxicity Of Metallointercalators Correlate With Cellular Uptake Or Dna Affinity?, Kimberley J. Davis, Judith A. Carrall, Barry Lai, Janice R. Aldrich-Wright, Stephen F. Ralph, Carolyn T. Dillon
Does Cytotoxicity Of Metallointercalators Correlate With Cellular Uptake Or Dna Affinity?, Kimberley J. Davis, Judith A. Carrall, Barry Lai, Janice R. Aldrich-Wright, Stephen F. Ralph, Carolyn T. Dillon
Faculty of Science - Papers (Archive)
The cytotoxicity of the metallointercalators, [Pt(5,6-dimethyl-1,10-phenanthroline)(trans-1R,2Rdiaminocyclohexane)] 2+ ([56MERR]) and [Pt(5,6-dimethyl-1,10-phenanthroline)(trans-1S,2Sdiaminocyclohexane)] 2+ ([56MESS]), towards A549 human lung cancer cells was examined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The IC50 value obtained following exposure of A549 cells to [56MESS] for 4 h was approximately three times smaller than that obtained when [56MERR] was administered under the same conditions, indicating that the former complex displayed greater cytotoxicity. Both IC50 values were greater than that obtained after exposure of A549 cells to cisplatin, demonstrating that the latter compound was the most cytotoxic of the three examined. Microprobe synchrotron radiation X-ray fluorescence (SR-XRF) analyses of …
Architecture And Conservation Of The Bacterial Dna Replication Machinery, An Underexploited Drug Target, Andrew Robinson, Rebecca J. Causer, Nicholas E. Dixon
Architecture And Conservation Of The Bacterial Dna Replication Machinery, An Underexploited Drug Target, Andrew Robinson, Rebecca J. Causer, Nicholas E. Dixon
Faculty of Science - Papers (Archive)
"New antibiotics with novel modes of action are required to combat the growing threat posed by multi-drug resistant bacteria. Over the last decade, genome sequencing and other high-throughput techniques have provided tremendous insight into the molecular processes underlying cellular functions in a wide range of bacterial species. We can now use these data to assess the degree of conservation of certain aspects of bacterial physiology, to help choose the best cellular targets for development of new broad-spectrum antibacterials. DNA replication is a conserved and essential process, and the large number of proteins that interact to replicate DNA in bacteria are …