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Statistical Analysis Of Real-Time Pcr Data, Joshua S. Yuan, Ann Reed, Feng Chen, C. Neal Stewart
Statistical Analysis Of Real-Time Pcr Data, Joshua S. Yuan, Ann Reed, Feng Chen, C. Neal Stewart
Faculty Publications and Other Works -- General Biology
Background
Even though real-time PCR has been broadly applied in biomedical sciences, data processing procedures for the analysis of quantitative real-time PCR are still lacking; specifically in the realm of appropriate statistical treatment. Confidence interval and statistical significance considerations are not explicit in many of the current data analysis approaches. Based on the standard curve method and other useful data analysis methods, we present and compare four statistical approaches and models for the analysis of real-time PCR data.
Results
In the first approach, a multiple regression analysis model was developed to derive ΔΔCt from estimation of interaction of gene and …